Antineoplastic effect of pectic polysaccharides from green sweet pepper (Capsicum annuum) on mammary tumor cells in vivo and in vitro

[Display omitted] •The polysaccharides from green sweet pepper (CAP) presented antineoplastic effects against mammary tumor lineages.•CAP treatment reduced the gene expression of VEGF in tumor cells in vivo and in vitro.•CAP treatment reduced the area of vessels in Ehrlich tumor.•CAP treatment induc...

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Vydáno v:Carbohydrate polymers Ročník 201; s. 280 - 292
Hlavní autoři: Adami, Eliana Rezende, Corso, Claudia Rita, Turin-Oliveira, Natalia Mulinari, Galindo, Claudia Martins, Milani, Letícia, Stipp, Maria Caroline, do Nascimento, Georgia Erdmann, Chequin, Andressa, da Silva, Luisa Mota, de Andrade, Sérgio Faloni, Dittrich, Rosangela Locatelli, Queiroz-Telles, José Ederaldo, Klassen, Giseli, Ramos, Edneia A.S., Cordeiro, Lucimara M.C., Acco, Alexandra
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Elsevier Ltd 01.12.2018
Témata:
ISSN:0144-8617, 1879-1344, 1879-1344
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Abstract [Display omitted] •The polysaccharides from green sweet pepper (CAP) presented antineoplastic effects against mammary tumor lineages.•CAP treatment reduced the gene expression of VEGF in tumor cells in vivo and in vitro.•CAP treatment reduced the area of vessels in Ehrlich tumor.•CAP treatment induced necrosis in Ehrlich solid tumors.•CAP treatment increased the levels of IL-6 in Ehrlich tumors in mice. The present study investigated the antineoplastic effects of pectic polysaccharides that were extracted from green sweet pepper (Capsicum annuum [CAP]) in the Ehrlich carcinoma in mice and in human mammary tumor lineages. After the subcutaneous inoculation of 2 × 106 Ehrlich tumor cells, Female Swiss mice received 50, 100, or 150 mg/kg CAP or vehicle orally once daily or methotrexate (2.5 mg/kg, i.p., every 5 days) for 21 days. CAP dose-dependently reduced Ehrlich tumor growth. It also reduced the viability of MCF-7, MDA-MB-231, and MDA-MB-436 human mammary cell lineages. Treatment with CAP reduced the gene expression of vascular endothelial growth factor in vivo and in vitro, reduced vessel areas of the tumors, and induced necrosis in Ehrlich solid tumors. CAP treatment significantly increased Interleukin-6 in tumors. The antineoplastic effect of CAP appears to depend on the regulation of inflammation and angiogenesis. Further studies are encouraged to better understand the CAP potential for the treatment of breast tumors.
AbstractList The present study investigated the antineoplastic effects of pectic polysaccharides that were extracted from green sweet pepper (Capsicum annuum [CAP]) in the Ehrlich carcinoma in mice and in human mammary tumor lineages. After the subcutaneous inoculation of 2 × 106 Ehrlich tumor cells, Female Swiss mice received 50, 100, or 150 mg/kg CAP or vehicle orally once daily or methotrexate (2.5 mg/kg, i.p., every 5 days) for 21 days. CAP dose-dependently reduced Ehrlich tumor growth. It also reduced the viability of MCF-7, MDA-MB-231, and MDA-MB-436 human mammary cell lineages. Treatment with CAP reduced the gene expression of vascular endothelial growth factor in vivo and in vitro, reduced vessel areas of the tumors, and induced necrosis in Ehrlich solid tumors. CAP treatment significantly increased Interleukin-6 in tumors. The antineoplastic effect of CAP appears to depend on the regulation of inflammation and angiogenesis. Further studies are encouraged to better understand the CAP potential for the treatment of breast tumors.The present study investigated the antineoplastic effects of pectic polysaccharides that were extracted from green sweet pepper (Capsicum annuum [CAP]) in the Ehrlich carcinoma in mice and in human mammary tumor lineages. After the subcutaneous inoculation of 2 × 106 Ehrlich tumor cells, Female Swiss mice received 50, 100, or 150 mg/kg CAP or vehicle orally once daily or methotrexate (2.5 mg/kg, i.p., every 5 days) for 21 days. CAP dose-dependently reduced Ehrlich tumor growth. It also reduced the viability of MCF-7, MDA-MB-231, and MDA-MB-436 human mammary cell lineages. Treatment with CAP reduced the gene expression of vascular endothelial growth factor in vivo and in vitro, reduced vessel areas of the tumors, and induced necrosis in Ehrlich solid tumors. CAP treatment significantly increased Interleukin-6 in tumors. The antineoplastic effect of CAP appears to depend on the regulation of inflammation and angiogenesis. Further studies are encouraged to better understand the CAP potential for the treatment of breast tumors.
[Display omitted] •The polysaccharides from green sweet pepper (CAP) presented antineoplastic effects against mammary tumor lineages.•CAP treatment reduced the gene expression of VEGF in tumor cells in vivo and in vitro.•CAP treatment reduced the area of vessels in Ehrlich tumor.•CAP treatment induced necrosis in Ehrlich solid tumors.•CAP treatment increased the levels of IL-6 in Ehrlich tumors in mice. The present study investigated the antineoplastic effects of pectic polysaccharides that were extracted from green sweet pepper (Capsicum annuum [CAP]) in the Ehrlich carcinoma in mice and in human mammary tumor lineages. After the subcutaneous inoculation of 2 × 106 Ehrlich tumor cells, Female Swiss mice received 50, 100, or 150 mg/kg CAP or vehicle orally once daily or methotrexate (2.5 mg/kg, i.p., every 5 days) for 21 days. CAP dose-dependently reduced Ehrlich tumor growth. It also reduced the viability of MCF-7, MDA-MB-231, and MDA-MB-436 human mammary cell lineages. Treatment with CAP reduced the gene expression of vascular endothelial growth factor in vivo and in vitro, reduced vessel areas of the tumors, and induced necrosis in Ehrlich solid tumors. CAP treatment significantly increased Interleukin-6 in tumors. The antineoplastic effect of CAP appears to depend on the regulation of inflammation and angiogenesis. Further studies are encouraged to better understand the CAP potential for the treatment of breast tumors.
The present study investigated the antineoplastic effects of pectic polysaccharides that were extracted from green sweet pepper (Capsicum annuum [CAP]) in the Ehrlich carcinoma in mice and in human mammary tumor lineages. After the subcutaneous inoculation of 2 × 10 Ehrlich tumor cells, Female Swiss mice received 50, 100, or 150 mg/kg CAP or vehicle orally once daily or methotrexate (2.5 mg/kg, i.p., every 5 days) for 21 days. CAP dose-dependently reduced Ehrlich tumor growth. It also reduced the viability of MCF-7, MDA-MB-231, and MDA-MB-436 human mammary cell lineages. Treatment with CAP reduced the gene expression of vascular endothelial growth factor in vivo and in vitro, reduced vessel areas of the tumors, and induced necrosis in Ehrlich solid tumors. CAP treatment significantly increased Interleukin-6 in tumors. The antineoplastic effect of CAP appears to depend on the regulation of inflammation and angiogenesis. Further studies are encouraged to better understand the CAP potential for the treatment of breast tumors.
The present study investigated the antineoplastic effects of pectic polysaccharides that were extracted from green sweet pepper (Capsicum annuum [CAP]) in the Ehrlich carcinoma in mice and in human mammary tumor lineages. After the subcutaneous inoculation of 2 × 10⁶ Ehrlich tumor cells, Female Swiss mice received 50, 100, or 150 mg/kg CAP or vehicle orally once daily or methotrexate (2.5 mg/kg, i.p., every 5 days) for 21 days. CAP dose-dependently reduced Ehrlich tumor growth. It also reduced the viability of MCF-7, MDA-MB-231, and MDA-MB-436 human mammary cell lineages. Treatment with CAP reduced the gene expression of vascular endothelial growth factor in vivo and in vitro, reduced vessel areas of the tumors, and induced necrosis in Ehrlich solid tumors. CAP treatment significantly increased Interleukin-6 in tumors. The antineoplastic effect of CAP appears to depend on the regulation of inflammation and angiogenesis. Further studies are encouraged to better understand the CAP potential for the treatment of breast tumors.
Author Klassen, Giseli
Galindo, Claudia Martins
Chequin, Andressa
Dittrich, Rosangela Locatelli
Acco, Alexandra
Adami, Eliana Rezende
Stipp, Maria Caroline
do Nascimento, Georgia Erdmann
Turin-Oliveira, Natalia Mulinari
de Andrade, Sérgio Faloni
Corso, Claudia Rita
Milani, Letícia
Queiroz-Telles, José Ederaldo
da Silva, Luisa Mota
Cordeiro, Lucimara M.C.
Ramos, Edneia A.S.
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Keywords Ehrlich solid tumor
Green sweet pepper
Pectic polysaccharide
VEGF
Mammary tumor cells
Interleukin-6
Language English
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Snippet [Display omitted] •The polysaccharides from green sweet pepper (CAP) presented antineoplastic effects against mammary tumor lineages.•CAP treatment reduced the...
The present study investigated the antineoplastic effects of pectic polysaccharides that were extracted from green sweet pepper (Capsicum annuum [CAP]) in the...
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SubjectTerms angiogenesis
Animals
Antineoplastic Agents, Phytogenic - chemistry
Antineoplastic Agents, Phytogenic - isolation & purification
Antineoplastic Agents, Phytogenic - pharmacology
breast neoplasms
Breast Neoplasms - drug therapy
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Capsicum - chemistry
Capsicum annuum
carcinoma
Carcinoma, Ehrlich Tumor - drug therapy
Carcinoma, Ehrlich Tumor - metabolism
Carcinoma, Ehrlich Tumor - pathology
Drug Screening Assays, Antitumor
Ehrlich solid tumor
epithelial cells
Female
females
Green sweet pepper
Humans
inflammation
Interleukin-6
laboratory animals
mammary glands
mammary neoplasms (animal)
Mammary Neoplasms, Experimental - drug therapy
Mammary Neoplasms, Experimental - metabolism
Mammary tumor cells
MCF-7 Cells
methotrexate
Mice
necrosis
neoplasm cells
Pectic polysaccharide
pectins
Pectins - chemistry
Pectins - isolation & purification
Pectins - pharmacology
sweet peppers
vascular endothelial growth factors
VEGF
viability
Title Antineoplastic effect of pectic polysaccharides from green sweet pepper (Capsicum annuum) on mammary tumor cells in vivo and in vitro
URI https://dx.doi.org/10.1016/j.carbpol.2018.08.071
https://www.ncbi.nlm.nih.gov/pubmed/30241820
https://www.proquest.com/docview/2111149576
https://www.proquest.com/docview/2131885133
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