Optical and non‐optical methods for detection and characterization of microparticles and exosomes

Microparticles and exosomes are cell‐derived microvesicles present in body fluids that play a role in coagulation, inflammation, cellular homeostasis and survival, intercellular communication, and transport. Despite increasing scientific and clinical interest, no standard procedures are available fo...

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Vydáno v:Journal of thrombosis and haemostasis Ročník 8; číslo 12; s. 2596 - 2607
Hlavní autoři: VAN DER POL, E., HOEKSTRA, A. G., STURK, A., OTTO, C., VAN LEEUWEN, T. G., NIEUWLAND, R.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Oxford, UK Blackwell Publishing Ltd 01.12.2010
Témata:
characterization
Exosomes
Flow Cytometry
Fluorescent Dyes
Humans
Light
Male
microparticles
Microscopy, Electron, Transmission
Microspheres
microvesicles
optical detection
Optics and Photonics
Reference Standards
Scattering, Radiation
Spectrometry, Fluorescence
ISSN:1538-7933, 1538-7836, 1538-7836
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Popis
Shrnutí:Microparticles and exosomes are cell‐derived microvesicles present in body fluids that play a role in coagulation, inflammation, cellular homeostasis and survival, intercellular communication, and transport. Despite increasing scientific and clinical interest, no standard procedures are available for the isolation, detection and characterization of microparticles and exosomes, because their size is below the reach of conventional detection methods. Our objective is to give an overview of currently available and potentially applicable methods for optical and non‐optical determination of the size, concentration, morphology, biochemical composition and cellular origin of microparticles and exosomes. The working principle of all methods is briefly discussed, as well as their applications and limitations based on the underlying physical parameters of the technique. For most methods, the expected size distribution for a given microvesicle population is determined. The explanations of the physical background and the outcomes of our calculations provide insights into the capabilities of each method and make a comparison possible between the discussed methods. In conclusion, several (combinations of) methods can detect clinically relevant properties of microparticles and exosomes. These methods should be further explored and validated by comparing measurement results so that accurate, reliable and fast solutions come within reach.
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ISSN:1538-7933
1538-7836
1538-7836
DOI:10.1111/j.1538-7836.2010.04074.x