Lineage Reprogramming of Fibroblasts into Proliferative Induced Cardiac Progenitor Cells by Defined Factors

Several studies have reported reprogramming of fibroblasts into induced cardiomyocytes; however, reprogramming into proliferative induced cardiac progenitor cells (iCPCs) remains to be accomplished. Here we report that a combination of 11 or 5 cardiac factors along with canonical Wnt and JAK/STAT si...

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Vydáno v:	Cell stem cell Ročník 18; číslo 3; s. 354
Hlavní autoři:	Lalit, Pratik A, Salick, Max R, Nelson, Daryl O, Squirrell, Jayne M, Shafer, Christina M, Patel, Neel G, Saeed, Imaan, Schmuck, Eric G, Markandeya, Yogananda S, Wong, Rachel, Lea, Martin R, Eliceiri, Kevin W, Hacker, Timothy A, Crone, Wendy C, Kyba, Michael, Garry, Daniel J, Stewart, Ron, Thomson, James A, Downs, Karen M, Lyons, Gary E, Kamp, Timothy J
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 03.03.2016
Témata:	Animals Cell Proliferation Cell Survival Cellular Reprogramming Cellular Reprogramming Techniques - methods Fibroblasts - cytology Fibroblasts - metabolism Mice Mice, Transgenic Myoblasts, Cardiac - cytology Myoblasts, Cardiac - metabolism Transcription Factors - biosynthesis Transcription Factors - genetics
ISSN:	1875-9777, 1875-9777
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Popis
Shrnutí:	Several studies have reported reprogramming of fibroblasts into induced cardiomyocytes; however, reprogramming into proliferative induced cardiac progenitor cells (iCPCs) remains to be accomplished. Here we report that a combination of 11 or 5 cardiac factors along with canonical Wnt and JAK/STAT signaling reprogrammed adult mouse cardiac, lung, and tail tip fibroblasts into iCPCs. The iCPCs were cardiac mesoderm-restricted progenitors that could be expanded extensively while maintaining multipotency to differentiate into cardiomyocytes, smooth muscle cells, and endothelial cells in vitro. Moreover, iCPCs injected into the cardiac crescent of mouse embryos differentiated into cardiomyocytes. iCPCs transplanted into the post-myocardial infarction mouse heart improved survival and differentiated into cardiomyocytes, smooth muscle cells, and endothelial cells. Lineage reprogramming of adult somatic cells into iCPCs provides a scalable cell source for drug discovery, disease modeling, and cardiac regenerative therapy.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1875-9777 1875-9777
DOI:	10.1016/j.stem.2015.12.001