Development and Assessment of a Quantitative Reverse Transcription-PCR Assay for Simultaneous Measurement of Four Amplicons

Background: High-throughput and forward-deployable biological dosimetry capabilities are required for tactical and medical decisions after radiologic events. We previously reported a quantitative reverse transcription (QRT)-PCR assay for human radiation-responsive gene targets using a whole-blood ex...

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Bibliographic Details
Published in:	Clinical chemistry (Baltimore, Md.) Vol. 49; no. 9; pp. 1467 - 1475
Main Authors:	Grace, Marcy B, McLeland, Christopher B, Gagliardi, Steven J, Smith, Jeffrey M, Jackson, William E., III, Blakely, William F
Format:	Journal Article
Language:	English
Published:	Washington, DC Am Assoc Clin Chem 01.09.2003 American Association for Clinical Chemistry Oxford University Press
Subjects:	bcl-2-Associated X Protein Biological and medical sciences Biomarkers Blood DNA Repair DNA, Complementary - chemistry DNA-Binding Proteins - blood DNA-Binding Proteins - genetics Dosimetry GADD45 Proteins Gene expression Humans Intracellular Signaling Peptides and Proteins Investigative techniques, diagnostic techniques (general aspects) Irradiation Laboratories Male Medical sciences Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Proteins - chemistry Proteins - genetics Proteins - metabolism Proto-Oncogene Proteins - blood Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins c-bcl-2 Radiation Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction - methods RNA - chemistry RNA, Ribosomal, 18S - blood RNA, Ribosomal, 18S - chemistry Superoxide Dismutase - blood Superoxide Dismutase - genetics Human RNA Blood Dose activity relation Gene Reference system Amplicon DNA Clinical biology Irradiation Genetics Technique Molecular biology Reverse transcription polymerase chain reaction Comparative study Quantitative analysis
ISSN:	0009-9147, 1530-8561
Online Access:	Get full text
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