Competitive reverse transcriptase-polymerase chain reaction shows that dietary zinc supplementation in humans increases monocyte metallothionein mRNA levels

Zinc status is difficult to evaluate in humans. Metallothionein gene expression is transcriptionally regulated by dietary zinc and thus could serve as an assessment parameter based on zinc-dependent function. We used semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to establ...

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Bibliographic Details
Published in:	The Journal of nutrition Vol. 127; no. 5; p. 694
Main Authors:	Sullivan, V K, Cousins, R J
Format:	Journal Article
Language:	English
Published:	United States 01.05.1997
Subjects:	Adult Analysis of Variance Base Sequence Blotting, Northern Cell Separation Cells, Cultured DNA, Complementary - analysis DNA, Complementary - chemistry DNA, Complementary - genetics Electrophoresis, Polyacrylamide Gel Flow Cytometry - methods Food, Fortified Gene Expression Regulation Gene Expression Regulation, Neoplastic Humans Leukemia, Myeloid - blood Leukemia, Myeloid - metabolism Leukemia, Myeloid - pathology Male Metallothionein - drug effects Metallothionein - genetics Metallothionein - metabolism Monocytes - chemistry Monocytes - drug effects Monocytes - metabolism Polymerase Chain Reaction - methods RNA, Messenger - analysis RNA, Messenger - genetics RNA, Messenger - metabolism RNA-Directed DNA Polymerase Tumor Cells, Cultured Zinc - administration & dosage Zinc - blood Zinc - pharmacology
ISSN:	0022-3166
Online Access:	Get more information
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