Reduced expression of cyclooxygenase (COX) in idiopathic pulmonary fibrosis and sarcoidosis

Aims:  To test the hypothesis that cyclooxygenase (COX)‐1 or COX‐2 expression is defective in lungs in idiopathic pulmonary fibrosis (IPF) and to characterize the cellular distribution. IPF is a progressive inflammatory lung disorder with an adverse prognosis. Previous work has shown that prostaglan...

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Veröffentlicht in:Histopathology Jg. 43; H. 4; S. 381 - 386
Hauptverfasser: Petkova, D K, Clelland, C A, Ronan, J E, Lewis, S, Knox, A J
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Oxford, UK Blackwell Science Ltd 01.10.2003
Blackwell
Schlagworte:
Adult
Aged
Biological and medical sciences
Bronchi - enzymology
Bronchi - pathology
cyclooxygenase
Cyclooxygenase 1
Cyclooxygenase 2
Female
Fluorescent Antibody Technique, Indirect
Humans
idiopathic pulmonary fibrosis
immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
Isoenzymes - metabolism
Macrophages, Alveolar - enzymology
Macrophages, Alveolar - pathology
Male
Medical sciences
Membrane Proteins
Middle Aged
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Pneumology
Prostaglandin-Endoperoxide Synthases - metabolism
Pulmonary Fibrosis - enzymology
Pulmonary Fibrosis - pathology
Respiratory Mucosa - enzymology
Respiratory Mucosa - pathology
Respiratory system : syndromes and miscellaneous diseases
sarcoidosis
Sarcoidosis, Pulmonary - enzymology
Sarcoidosis, Pulmonary - pathology
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Prostaglandin-endoperoxide synthase
Anatomic pathology
Pulmonary fibrosis
Sarcoidosis
Enzyme
Systemic disease
Idiopathic
cyclooxygenase
Oxidoreductases
idiopathic pulmonary fibrosis
immunohistochemistry
ISSN:0309-0167, 1365-2559
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Zusammenfassung:Aims:  To test the hypothesis that cyclooxygenase (COX)‐1 or COX‐2 expression is defective in lungs in idiopathic pulmonary fibrosis (IPF) and to characterize the cellular distribution. IPF is a progressive inflammatory lung disorder with an adverse prognosis. Previous work has shown that prostaglandin E2 (PGE2) regulates collagen deposition and fibroblast proliferation and a defect in COX regulation may contribute to the fibrosis that occurs in IPF. Methods:  Immunohistochemistry was utilized to determine COX immunoreactivity in lung sections from 25 IPF, six sarcoidosis and 14 control subjects. Results:  COX‐1 and COX‐2 expression in bronchiolar epithelial cells was significantly lower in IPF and sarcoidosis than in controls. No significant difference was found in COX‐2 expression between macrophages in IPF and control sections, but COX‐2 was reduced in macrophages in sarcoidosis compared with controls. Conclusions:  These studies confirm COX‐2 loss in bronchial epithelial cells but not macrophages in IPF, and show for the first time reduced constitutive COX‐1 expression in epithelial cells and macrophages. Similar abnormalities were observed in sarcoidosis.
Bibliographie:istex:EE609D0062D92E20544CAE9BBE5F4AC7D57B59E8
ark:/67375/WNG-SGDQF950-X
ArticleID:HIS1718
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0309-0167
1365-2559
DOI:10.1046/j.1365-2559.2003.01718.x