Replication of nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda: microscopy study of the sequence of events of the virus infection

A microscopy study of the sequence of morphogenic events of Spodoptera frugiperda nuclear polyhedrosis virus infection of S. frugiperda cells is presented which orders the sequence of replication and establishes the time scale within which the events occur. The virus entered the cell by 1 h postinfe...

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Bibliographic Details
Published in:Journal of virology Vol. 17; no. 1; p. 254
Main Authors: Knudson, D L, Harrap, K A
Format: Journal Article
Language:English
Published: United States 1976
Subjects:
Adsorption
Cell Line
Cell Membrane - microbiology
Insect Viruses - growth & development
Insect Viruses - ultrastructure
Insecta - microbiology
Morphogenesis
Vacuoles - microbiology
Virus Replication
ISSN:0022-538X
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Summary:A microscopy study of the sequence of morphogenic events of Spodoptera frugiperda nuclear polyhedrosis virus infection of S. frugiperda cells is presented which orders the sequence of replication and establishes the time scale within which the events occur. The virus entered the cell by 1 h postinfection and was uncoated. The eclipse period was 9 h and the latent period was 12 h. Polyhedron formation was detected by 18 h postinfection and continued until the deposition of the polyhedron membrane was completed by 48 h postinfection. Aberrant morphogenic characteristics of virus repeatedly passaged in the cell culture were also recorded. Adsorption, envelope morphogenesis, and release mechanisms are discussed in light of other data on in vivo and in vitro baculovirus infections.
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ISSN:0022-538X
DOI:10.1128/jvi.17.1.254-268.1976