Optimal reprogramming factor stoichiometry increases colony numbers and affects molecular characteristics of murine induced pluripotent stem cells

Somatic cells can be reprogrammed toward pluripotency by overexpression of a set of transcription factors, yielding induced pluripotent stem cells (iPSCs) with features similar to embryonic stem cells. Little is known to date about stoichiometric requirements of the individual reprogramming factors...

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Bibliographic Details
Published in:	Cytometry. Part A Vol. 79A; no. 6; pp. 426 - 435
Main Authors:	Tiemann, Ulf, Sgodda, Malte, Warlich, Eva, Ballmaier, Matthias, Schöler, Hans R., Schambach, Axel, Cantz, Tobias
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.2011
Subjects:	Alkaline Phosphatase - metabolism Animals c-Myc protein Cell Count Cell Differentiation Cells, Cultured Cellular Reprogramming - genetics Colonies Cytometry Embryo cells Embryo fibroblasts Fibroblasts - cytology Fibroblasts - metabolism Flow Cytometry Fluorescence fluorophores Gene Expression Gene Expression Profiling induced pluripotent stem cells Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Inhibitory postsynaptic potentials KLF4 protein Kruppel-Like Transcription Factors - genetics Kruppel-Like Transcription Factors - metabolism Lentivirus - genetics Mice multicolor flow cytometry Oct-4 protein Oct4 Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism Proto-Oncogene Proteins c-myc - genetics Proto-Oncogene Proteins c-myc - metabolism reprogramming efficiency Somatic cells SOXB1 Transcription Factors - genetics SOXB1 Transcription Factors - metabolism Stem cells stoichiometry Transcription factors Transfection
ISSN:	1552-4922, 1552-4930, 1552-4930
Online Access:	Get full text
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