Effect of antiprogestin RU 486 on the proliferation and metabolic activity of stromal cells separated from human endometrium

The effects of RU 486 on the proliferation and metabolic activity of human endometrial stromal cells in culture were studied. RU 486 at 10 −7 M/well significantly stimulated (P<0.001) the growth as well as metabolic activity in the culture system. Interestingly, RU 486 at 10 −6 M/well did not sti...

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Bibliographic Details
Published in:Contraception (Stoneham) Vol. 53; no. 2; pp. 127 - 131
Main Authors: Bhargava-Periwal, S., Farooq, A., Bhargava, V.L., Bhatla, N., Vij, U., Murugesan, K.
Format: Journal Article
Language:English
Published: New York, NY Elsevier Inc 01.02.1996
Elsevier Science
Subjects:
Biological and medical sciences
Birth control
Cell Division - drug effects
Cells, Cultured
endometrium
Endometrium - cytology
Female
Gynecology. Andrology. Obstetrics
Hormone Antagonists - pharmacology
human
Humans
Medical sciences
Mifepristone - pharmacology
Other methods of contraception. Sterilization
progesterone
Progesterone - antagonists & inhibitors
Progesterone - pharmacology
RU 486
stromal cells
Stromal Cells - cytology
Stromal Cells - drug effects
Stromal Cells - metabolism
endometrium
stromal cells
human
progesterone
RU 486
Human
Cell proliferation
Abortifacient
Female
Stromal cell
Mechanism of action
Endometrium
Genitalia
Endometrial Effects
In Vitro
Biology
Hormones
Research Methodology
Endocrine System
Genitalia, Female
Urogenital System
Clinical Research
Uterus
Research Report
Physiology
Hormone Antagonists
Ru-486
Metabolic Effects
ISSN:0010-7824, 1879-0518
Online Access:Get full text
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Summary:The effects of RU 486 on the proliferation and metabolic activity of human endometrial stromal cells in culture were studied. RU 486 at 10 −7 M/well significantly stimulated (P<0.001) the growth as well as metabolic activity in the culture system. Interestingly, RU 486 at 10 −6 M/well did not stimulate metabolic activity in the culture. Progesterone, in combination with RU 486 at 10 −7 M/well, caused a significant increase in proliferation (assessed by thymidine incorporation) (P<0.001) over control and P 4 alone, but not significantly different from RU 486 at 10 −7 M/well alone. The same pattern was observed for metabolic functions (assessed by uridine incorporation) when RU 486 at 10 −7 M/well, along with P 4, was added to the culture. Interestingly, RU 486 at 10 −6 M/well with P 4 had no effect on RNA synthesis in the culture. The relevance of these findings is discussed.
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content type line 23
ISSN:0010-7824
1879-0518
DOI:10.1016/0010-7824(95)00262-6