Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP)

Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	Beni-Suef University journal of basic and applied sciences Ročník 14; číslo 1; s. 42 - 8
Hlavní autori:	Abdellatif, Manal Z. M., Abdel-Hafeez, Ekhlas H., Belal, Usama S., Abdelgelil, Noha H., Marey, Heba, Nasr, Manar M. S. A., Abdel-Fatah, Mohamed Mahmoud
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Berlin/Heidelberg Springer Berlin Heidelberg 30.04.2025 Springer Nature B.V SpringerOpen
Predmet:	Blastocystis Blastocystis spp Cancer Cysts Diabetes Diabetes mellitus Diarrhea Drinking water Enzymes Females Grouping Immuno-compromised Immunocompetence Immunocompromised hosts Infections Intestinal parasites Kidney diseases Medicine Medicine & Public Health Parasites Parasitic diseases Parasitology PCR–RFLP Polymerase chain reaction Polymorphism Protozoa Restriction fragment length polymorphism rRNA United States > US spp Immuno-compromised Grouping PCR–RFLP
ISSN:	2314-8543, 2314-8535, 2314-8543
On-line prístup:	Získať plný text
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Abstract	Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations. Materials and methods A cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping. Results Among 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference ( P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases. Conclusion Blastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation.
AbstractList	BackgroundBlastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations.Materials and methodsA cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping.ResultsAmong 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference (P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases.ConclusionBlastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation. Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations. Materials and methods A cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping. Results Among 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference ( P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases. Conclusion Blastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation. Abstract Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations. Materials and methods A cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping. Results Among 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference (P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases. Conclusion Blastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation.
ArticleNumber	42
Author	Abdel-Hafeez, Ekhlas H. Abdellatif, Manal Z. M. Belal, Usama S. Marey, Heba Abdelgelil, Noha H. Nasr, Manar M. S. A. Abdel-Fatah, Mohamed Mahmoud
Author_xml	– sequence: 1 givenname: Manal Z. M. surname: Abdellatif fullname: Abdellatif, Manal Z. M. organization: Minia University – sequence: 2 givenname: Ekhlas H. surname: Abdel-Hafeez fullname: Abdel-Hafeez, Ekhlas H. organization: Minia University – sequence: 3 givenname: Usama S. surname: Belal fullname: Belal, Usama S. organization: Minia University – sequence: 4 givenname: Noha H. surname: Abdelgelil fullname: Abdelgelil, Noha H. organization: Minia University – sequence: 5 givenname: Heba surname: Marey fullname: Marey, Heba organization: Minia University – sequence: 6 givenname: Manar M. S. A. surname: Nasr fullname: Nasr, Manar M. S. A. organization: Minia University – sequence: 7 givenname: Mohamed Mahmoud surname: Abdel-Fatah fullname: Abdel-Fatah, Mohamed Mahmoud email: mo.abdelfatah@mu.edu.eg organization: Minia University
BookMark	eNp9UctuFDEQtFCQCEt-gJMlLomUAXs8zyMEApEigRCcrR5Pe9erGXtwew-bT-IrcTIgEIf40larqrq76jk78cEjYy-leC1l17yhSomuK0RZF0I0ShZ3T9hpqWRVdHWlTv75P2NnRHshhOxLIUt1yn6-x4QmueB5sPzdBJSCOVJyxGlB45C489zN88EHE-YlhtkRjnyB5NAn4ucGvMF4yUcHQ9YiPuM0uXSgSw5-5GYXg3eGR_QwZRAhENIFH465RSm6dbiNsJ2zIJ_Qb9OOL2E6ziEuO0czP_96ffvl4gV7amEiPPtdN-z79YdvV5-K288fb67e3hamUjIVQ9mq0YraNnYwthlEPXamrdu-lnYA2ZgGQJgSZDuMJrslbKt6NJU0Qy_bFtSG3ay6Y4C9XqKbIR51AKcfGiFuNcTkzIRatNXY2soIWWGFou-y06pXOEAt66Zus9arVSsb9-OQ79X7cIjZCdKqzFDRqfw2rFtRJgaiiFYbl-DelxTBTVoKfR-0XoPWOWj9ELS-y9TyP-qfhR8lqZVEGey3GP9u9QjrF-h6wPY
CitedBy_id	crossref_primary_10_2147_IMCRJ_S532766 crossref_primary_10_1186_s13099_025_00733_0
Cites_doi	10.2478/s11686-018-00002-y 10.1016/j.parepi.2020.e00134 10.1093/cid/cir810 10.1007/s11356-021-16187-1 10.1371/journal.pone.0090972 10.21767/2386-5180.100092 10.1007/s11686-021-00360-0 10.1016/j.parint.2016.02.010 10.1007/s00436-007-0808-0 10.1007/s00436-009-1551-5 10.1590/S1413-86702008000400017 10.1177/2049936113504754 10.1159/000049072 10.1007/s00436-009-1595-6 10.1017/S0950268809002672 10.1007/s11908-009-0073-8 10.1089/fpd.2012.1177 10.2147/IDR.S347220 10.1016/j.gmbhs.2013.05.003 10.1093/milmed/167.8.643 10.1023/A:1007551218671 10.1128/jcm.22.4.666-667.1985 10.2174/1381612824666180807101536 10.1007/s00436-011-2342-3 10.1007/s00436-009-1485-y 10.3855/jidc.12650 10.15406/emij.2019.07.00265 10.1007/s00436-012-3218-x 10.1007/s00436-016-4951-3 10.1046/j.1525-1594.2003.07215.x 10.1128/CMR.00022-08 10.1007/s00436-008-1031-3 10.1016/j.parint.2010.03.009 10.2147/RMHP.S269664 10.21608/jesp.2020.131115 10.1186/1471-2334-13-389 10.1016/j.micpath.2024.106876 10.2478/s11686-020-00186-2 10.1016/j.parint.2016.05.015
ContentType	Journal Article
Copyright	The Author(s) 2025 Copyright Springer Nature B.V. Dec 2025
Copyright_xml	– notice: The Author(s) 2025 – notice: Copyright Springer Nature B.V. Dec 2025
DBID	C6C AAYXX CITATION 8FE 8FG 8FH ABJCF ABUWG AFKRA AZQEC BBNVY BENPR BGLVJ BHPHI CCPQU DWQXO GNUQQ HCIFZ L6V LK8 M7P M7S PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI PRINS PTHSS DOA
DOI	10.1186/s43088-025-00631-z
DatabaseName	Springer Nature OA Free Journals CrossRef ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials - QC Biological Science Collection ProQuest Central (New) (NC LIVE) Technology collection Natural Science Collection ProQuest One Community College ProQuest Central ProQuest Central Student SciTech Premium Collection ProQuest Engineering Collection Biological Sciences Biological Science Database Engineering Database ProQuest Central Premium ProQuest One Academic (New) ProQuest Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Engineering collection DOAJ Directory of Open Access Journals
DatabaseTitle	CrossRef Publicly Available Content Database ProQuest Central Student Technology Collection ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences ProQuest Engineering Collection Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Central (New) Engineering Collection Engineering Database ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Technology Collection Biological Science Database ProQuest SciTech Collection ProQuest One Academic UKI Edition Materials Science & Engineering Collection ProQuest One Academic ProQuest One Academic (New)
DatabaseTitleList	Publicly Available Content Database
Database_xml	– sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Medicine
EISSN	2314-8543
EndPage	8
ExternalDocumentID	oai_doaj_org_article_074d7f4c014e4e098231393eba515657 10_1186_s43088_025_00631_z
GeographicLocations	United States--US
GeographicLocations_xml	– name: United States--US
GroupedDBID	0R~ 4.4 457 5VS AAEDT AAFWJ AAIKJ AAKKN ABEEZ ABJCF ABMAC ACACY ACGFS ACULB ADBBV ADEZE AFGXO AFKRA AFPKN AGHFR AKRWK ALMA_UNASSIGNED_HOLDINGS BBNVY BCNDV BENPR BGLVJ BHPHI C24 C6C CCPQU EBS FDB GROUPED_DOAJ HCIFZ IAO IGS ITC IXB KQ8 M7P M7S OK1 PHGZM PHGZT PIMPY PQGLB PTHSS SOJ AAYXX AFFHD CITATION 8FE 8FG 8FH ABUWG AZQEC DWQXO GNUQQ L6V LK8 PKEHL PQEST PQQKQ PQUKI PRINS
ID	FETCH-LOGICAL-c431t-b273df05f6fbcf6b05d8c757951fba16c6aa0c2a17bdc6310f739ec41cb9177a3
IEDL.DBID	DOA
ISICitedReferencesCount	2
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=001479423500001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	2314-8543 2314-8535
IngestDate	Fri Oct 03 12:39:48 EDT 2025 Tue Oct 07 13:10:44 EDT 2025 Sat Nov 29 08:01:04 EST 2025 Tue Nov 18 20:49:48 EST 2025 Mon Jul 21 06:22:11 EDT 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	1
Keywords	spp Immuno-compromised Grouping PCR–RFLP
Language	English
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c431t-b273df05f6fbcf6b05d8c757951fba16c6aa0c2a17bdc6310f739ec41cb9177a3
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14
OpenAccessLink	https://doaj.org/article/074d7f4c014e4e098231393eba515657
PQID	3213908333
PQPubID	5642984
PageCount	8
ParticipantIDs	doaj_primary_oai_doaj_org_article_074d7f4c014e4e098231393eba515657 proquest_journals_3213908333 crossref_citationtrail_10_1186_s43088_025_00631_z crossref_primary_10_1186_s43088_025_00631_z springer_journals_10_1186_s43088_025_00631_z
PublicationCentury	2000
PublicationDate	2025-04-30
PublicationDateYYYYMMDD	2025-04-30
PublicationDate_xml	– month: 04 year: 2025 text: 2025-04-30 day: 30
PublicationDecade	2020
PublicationPlace	Berlin/Heidelberg
PublicationPlace_xml	– name: Berlin/Heidelberg – name: Beni Suef Governorate
PublicationTitle	Beni-Suef University journal of basic and applied sciences
PublicationTitleAbbrev	Beni-Suef Univ J Basic Appl Sci
PublicationYear	2025
Publisher	Springer Berlin Heidelberg Springer Nature B.V SpringerOpen
Publisher_xml	– name: Springer Berlin Heidelberg – name: Springer Nature B.V – name: SpringerOpen
References	SM Eassa (631_CR2) 2016; 4 631_CR30 A Asghari (631_CR3) 2021; 66 F Dogruman-Al (631_CR11) 2008; 103 C Vogelberg (631_CR34) 2010; 59 KS Tan (631_CR33) 2008; 21 T Sanaka (631_CR28) 2003; 27 EH Abdel-Hafeez (631_CR5) 2016; 115 I Wawrzyniak (631_CR1) 2013; 1 F Eroglu (631_CR13) 2009; 105 C Stensvold (631_CR14) 2009; 137 S Gabrielli (631_CR16) 2020; 9 P Taamasri (631_CR32) 2002; 167 F Cakir (631_CR10) 2019; 64 O Cirioni (631_CR35) 1999; 15 H Yoshikawa (631_CR21) 2011; 109 M Şahin (631_CR38) 2023; 18 CR Stensvold (631_CR31) 2016; 65 L Karthik (631_CR6) 2014; 9 Z Drawany (631_CR39) 2019; 7 N Turkcapar (631_CR26) 2002; 90 RA Kulik (631_CR27) 2008; 12 S Aydemir (631_CR7) 2024; 195 631_CR25 631_CR24 FO Akinbo (631_CR40) 2013; 5 CM Coyle (631_CR22) 2012; 54 O Yersal (631_CR15) 2016; 65 KS Tan (631_CR18) 2010; 12 MV Domínguez-Márquez (631_CR12) 2009; 105 EG Estevez (631_CR19) 1985; 22 S Jantermtor (631_CR17) 2013; 112 A Asghari (631_CR8) 2021; 28 V Kumarasamy (631_CR41) 2018; 24 MH Wakid (631_CR4) 2022; 15 A Bart (631_CR9) 2013; 13 SS Ibrahim (631_CR36) 2020; 50 A Asghari (631_CR29) 2020; 65 T Tan (631_CR23) 2009; 105 P Laodim (631_CR37) 2012; 9 KH Wong (631_CR20) 2008; 102 B Gulhan (631_CR42) 2020; 14
References_xml	– volume: 64 start-page: 7 year: 2019 ident: 631_CR10 publication-title: Acta Parasitol doi: 10.2478/s11686-018-00002-y – volume: 9 year: 2020 ident: 631_CR16 publication-title: Parasite Epidemiol Control doi: 10.1016/j.parepi.2020.e00134 – volume: 54 start-page: 105 year: 2012 ident: 631_CR22 publication-title: Clin Infect Dis doi: 10.1093/cid/cir810 – volume: 28 start-page: 60308 year: 2021 ident: 631_CR8 publication-title: Environ Sci Pollut Res doi: 10.1007/s11356-021-16187-1 – volume: 9 year: 2014 ident: 631_CR6 publication-title: PLoS ONE doi: 10.1371/journal.pone.0090972 – ident: 631_CR25 – volume: 4 start-page: 92 year: 2016 ident: 631_CR2 publication-title: Ann Clin Lab Res doi: 10.21767/2386-5180.100092 – volume: 66 start-page: 745 year: 2021 ident: 631_CR3 publication-title: Acta Parasitol doi: 10.1007/s11686-021-00360-0 – volume: 65 start-page: 792 year: 2016 ident: 631_CR15 publication-title: Parasitol Int doi: 10.1016/j.parint.2016.02.010 – volume: 102 start-page: 663 year: 2008 ident: 631_CR20 publication-title: Parasitol Res doi: 10.1007/s00436-007-0808-0 – volume: 105 start-page: 1283 year: 2009 ident: 631_CR23 publication-title: Parasitol Res doi: 10.1007/s00436-009-1551-5 – volume: 12 start-page: 338 year: 2008 ident: 631_CR27 publication-title: Brazil J Infect Dis doi: 10.1590/S1413-86702008000400017 – volume: 1 start-page: 167 year: 2013 ident: 631_CR1 publication-title: Therapeutic Adv Infect Dis doi: 10.1177/2049936113504754 – volume: 90 start-page: 344 year: 2002 ident: 631_CR26 publication-title: Nephron doi: 10.1159/000049072 – volume: 105 start-page: 1589 year: 2009 ident: 631_CR13 publication-title: Parasitol Res doi: 10.1007/s00436-009-1595-6 – volume: 137 start-page: 1655 year: 2009 ident: 631_CR14 publication-title: Epidemiol Infect doi: 10.1017/S0950268809002672 – volume: 12 start-page: 28 year: 2010 ident: 631_CR18 publication-title: Curr Infect Dis Rep doi: 10.1007/s11908-009-0073-8 – volume: 9 start-page: 1077 year: 2012 ident: 631_CR37 publication-title: Foodborne Pathog Dis doi: 10.1089/fpd.2012.1177 – volume: 15 start-page: 491 year: 2022 ident: 631_CR4 publication-title: Infect Drug Resistance doi: 10.2147/IDR.S347220 – volume: 5 start-page: 44 year: 2013 ident: 631_CR40 publication-title: Biomarkers Genom Med doi: 10.1016/j.gmbhs.2013.05.003 – volume: 167 start-page: 643 year: 2002 ident: 631_CR32 publication-title: Thailand Military Med doi: 10.1093/milmed/167.8.643 – volume: 15 start-page: 387 year: 1999 ident: 631_CR35 publication-title: Eur J Epidemiol doi: 10.1023/A:1007551218671 – volume: 22 start-page: 666 year: 1985 ident: 631_CR19 publication-title: J Clin Microbiol doi: 10.1128/jcm.22.4.666-667.1985 – volume: 24 start-page: 3172 year: 2018 ident: 631_CR41 publication-title: Curr Pharm Des doi: 10.2174/1381612824666180807101536 – volume: 109 start-page: 1045 year: 2011 ident: 631_CR21 publication-title: Parasitol Res doi: 10.1007/s00436-011-2342-3 – volume: 105 start-page: 949 year: 2009 ident: 631_CR12 publication-title: Parasitol Res doi: 10.1007/s00436-009-1485-y – volume: 14 start-page: 1448 year: 2020 ident: 631_CR42 publication-title: J Infect Dev Countries doi: 10.3855/jidc.12650 – volume: 7 start-page: 171 year: 2019 ident: 631_CR39 publication-title: Endocrinol Metab Int J doi: 10.15406/emij.2019.07.00265 – volume: 112 start-page: 1781 year: 2013 ident: 631_CR17 publication-title: Parasitol Res doi: 10.1007/s00436-012-3218-x – ident: 631_CR24 – volume: 115 start-page: 2061 year: 2016 ident: 631_CR5 publication-title: Parasitol Res doi: 10.1007/s00436-016-4951-3 – volume: 27 start-page: 224 year: 2003 ident: 631_CR28 publication-title: Artif Organs doi: 10.1046/j.1525-1594.2003.07215.x – volume: 21 start-page: 639 year: 2008 ident: 631_CR33 publication-title: Clin Microbiol Rev doi: 10.1128/CMR.00022-08 – volume: 103 start-page: 685 year: 2008 ident: 631_CR11 publication-title: Parasitol Res doi: 10.1007/s00436-008-1031-3 – volume: 18 start-page: 202 year: 2023 ident: 631_CR38 publication-title: Iran J Parasitol – volume: 59 start-page: 469 year: 2010 ident: 631_CR34 publication-title: Parasitol Int doi: 10.1016/j.parint.2010.03.009 – ident: 631_CR30 doi: 10.2147/RMHP.S269664 – volume: 50 start-page: 683 year: 2020 ident: 631_CR36 publication-title: J Egypt Soc Parasitol doi: 10.21608/jesp.2020.131115 – volume: 13 start-page: 1 year: 2013 ident: 631_CR9 publication-title: BMC Infect Dis doi: 10.1186/1471-2334-13-389 – volume: 195 year: 2024 ident: 631_CR7 publication-title: Microb Pathog doi: 10.1016/j.micpath.2024.106876 – volume: 65 start-page: 462 year: 2020 ident: 631_CR29 publication-title: Acta Parasitol doi: 10.2478/s11686-020-00186-2 – volume: 65 start-page: 763 year: 2016 ident: 631_CR31 publication-title: Parasitol Int doi: 10.1016/j.parint.2016.05.015
SSID	ssj0001920123 ssib044731334 ssib052605897
Score	2.3039823
Snippet	Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans... BackgroundBlastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans... Abstract Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from...
SourceID	doaj proquest crossref springer
SourceType	Open Website Aggregation Database Enrichment Source Index Database Publisher
StartPage	42
SubjectTerms	Blastocystis Blastocystis spp Cancer Cysts Diabetes Diabetes mellitus Diarrhea Drinking water Enzymes Females Grouping Immuno-compromised Immunocompetence Immunocompromised hosts Infections Intestinal parasites Kidney diseases Medicine Medicine & Public Health Parasites Parasitic diseases Parasitology PCR–RFLP Polymerase chain reaction Polymorphism Protozoa Restriction fragment length polymorphism rRNA
SummonAdditionalLinks	– databaseName: Biological Science Database dbid: M7P link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELagcODCQ4BYKMgHDq3YqJu1YzsnRIEVB1StEEi9WY4fZVE3WZIUafuT-JXMOM5WRaIXrnnZ0Xwef-Ox5yPktTdeeYniLhIiHQ4xRKbmXGRlKJQwuQ2-NFFsQp6cqNPTcpkW3Lq0rXL0idFRu8biGvkRmwNXAb7A2NvNzwxVozC7miQ0bpM7WCWBxa17yxFPnEsGIdgObwVyd5WyZj8GdoOUIurP5dDJghXjuRoljjrOYAhmqPeKE3meXV6bu2KJ_2u89K9UapyhFg_-998ekvuJm9J3A5gekVu-fkx-f_B93K5V0ybQYyDbfWO34Bg6iqc0IdCmq5qu8JhJg_vT2waQ4x1NBVs7emARWO2Ujsu8dI1FQPuLbkpN7agdyvPS1mPjKWHUHdJqS1E3pF0NjYfWnOFKJkXpl_473TTn23UDKFl1a3rwZfF5efiEfFt8_Pr-U5YEHjILvKXPKuBOLsyKIEJlgwALOWVlIYH1hcrkwgpjZnZuclk5CxaYBclKb3luK4gypWFPyV7d1P4ZoQEsxq0TEkzMc8NNxedOlqoEQqSKyk1IPppO21T9HEU4znWMgpTQg7k1mFtHc-vLCXmze2cz1P648eljRMTuSazbHS807ZlObkADYXMycAuBqeceugbAYyXzlQFeKQo5IfsjQnRyJp2-gseETEeMXd3-d5ee3_y1F-TePKIbc2P7ZK9vL_xLctf-Agi1r-JQ-gPefSKH priority: 102 providerName: ProQuest – databaseName: SpringerLINK dbid: C24 link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELagIMSFN2KhoDlwaMVGbBLHdo60sOIAVYVA6s3ys13UTaokRdr-JH4lY8cpKgIkuCa2Msp8Y3_j8cwQ8tIpJxwPzV04ejoUfYhMFJRlta8EU7nxrlax2QQ_OBBHR_VhSgrrp9vuU0gyrtTRrAV73dMSLSIL7VfDvppnF9fJjSoXdcD1fspx-DpylkAUpgyZ3069sgvFYv1XGOYvQdG41yzv_p-U98idxC3hzQiG--Saax6QWx9T9Pwh-f7WDfHmVQOthz3kzUNrNmjjPYSES_SZYdXAKmSMtOGqedciCJyFVHu1hx0TMNLNYTqxhXWo5zmc93NQjQUzVtqFzgU5Uuyn3wW9gdACpFuNH_edOg6HkhC6uAwncNaebtYtKnzVr2Hn0_LD4e4j8mX57vP--yz1asgMUpAh00iDrF9UnnltPNOLygrDK44EzmuVM8OUWphC5Vxbg_9l4XlZO0Nzo9Fh5Kp8TLaatnFPCHj8j9RYxtHTormiStPC8lrUyG1Epe2M5JPupEmFzEM_jVMZHRrB5KgEiUqQUQnyYkZeXc45G8t4_HX0XoDE5chQgjs-aLtjmSxaIvey3FODPqajDkVDplzWpdMKKSKr-IxsT4CSaV3oZVngGGS9ZTkj8wlAP1__WaSn_zb8GbldRAyGsNc22Rq6c_ec3DTfEFLdi2gvPwCO8BMU priority: 102 providerName: Springer Nature
Title	Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP)
URI	https://link.springer.com/article/10.1186/s43088-025-00631-z https://www.proquest.com/docview/3213908333 https://doaj.org/article/074d7f4c014e4e098231393eba515657
Volume	14
WOSCitedRecordID	wos001479423500001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2314-8543 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001920123 issn: 2314-8543 databaseCode: DOA dateStart: 20190101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2314-8543 dateEnd: 99991231 omitProxy: false ssIdentifier: ssib044731334 issn: 2314-8543 databaseCode: M~E dateStart: 20120101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 2314-8543 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001920123 issn: 2314-8543 databaseCode: M7P dateStart: 20191201 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database customDbUrl: eissn: 2314-8543 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001920123 issn: 2314-8543 databaseCode: M7S dateStart: 20191201 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central (subscription) customDbUrl: eissn: 2314-8543 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001920123 issn: 2314-8543 databaseCode: BENPR dateStart: 20191201 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2314-8543 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001920123 issn: 2314-8543 databaseCode: PIMPY dateStart: 20191201 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVAVX databaseName: SpringerLINK customDbUrl: eissn: 2314-8543 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001920123 issn: 2314-8543 databaseCode: C24 dateStart: 20191201 isFulltext: true titleUrlDefault: https://link.springer.com/search?facet-content-type=%22Journal%22 providerName: Springer Nature
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELagcOCCQIBYKCsfOLRio-bh2MmxW3YFEqyiAlI5WbZjw6JuUiVppe2BH8SvZMZJSosEXLjkkExkax6ab-x5EPLSKptZgcNdBEQ6DGKIIIsZD3KXZlxFxtlc-WETYrXKTk7y4tqoL8wJ69sD94w7ABdXCscMQHnLbJjjtVWSJ1Yr8MQ89XXkgHquBVPfetyCYGGsksn4QcsSMKgAp7eiW46CyxueyDfsv4Eyf7sY9f5m-YDcH4AiPew3-JDcstUj8uO17XzuVEVrR-eAfLvabMFKW4olkxD10nVF11jzUWOyeFODGG1Jh-6pLd0zKOVmRsczV7rBjpzdeTujqiqp6Xvl0sbi4sPtTbtP9ZbiEI9m3S_uGvUFjxUpzmHpvtKz-nS7qUFk63ZD946X74r9x-TTcvHx6E0wTFsIDICILtAAZEoXpo47bRzXYVpmRqQCIJjTKuKGKxWaWEVClwYYGDqR5NawyGgI-YRKnpCdqq7sU0IdMJyZkguIlVikmNIsLkWe5YBOslSXExKNnJdmaEWOEzFOpQ9JMi57aUmQlvTSkpcT8urqn7O-Ecdfqeco0CtKbKLtX4BqyUG15L9Ua0J2R3WQg2W3MomBBnBrkkzIbFSRX5__vKVn_2NLz8m92KswXmftkp2uObcvyF1zAYrWTMmd-WJVHE_J7aOYTb1FTDGZtfDPD_j8vgCq4u374vNP4J0RRQ
linkProvider	Directory of Open Access Journals
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1db9MwFLVGhwQvfAgQhQF-AGkTjdYkju08IMQY1aZ1VYWGNJ6M49ijaE1KkoG6n8QDv5F789FpSOxtD7y2aRK5516f42vfQ8hLq620As1dBCgdBhrCkwHjXuwiybVvnI11bTYhJhN5fBxP18jv7iwMbqvscmKdqNPc4Br5dhgAVwG-EIZvF989dI3C6mpnodHA4sAuf4JkK9_s78L_-yoIRh-O3u95rauAZ2CyrLwEJuzUDSPHXWIcT4ZRKo2IBFANl2ifG6710ATaF0lqOLAfJ8LYGuabBKSN0CHc9wZZZwB22SPr0_3D6ecOwYyJEETfCuERqgXZ1um-NXwKSUzteOfDsERh1J3kkXy7ZCEEvYcOs0gdfO_80mxZmwpcYsJ_FW_rOXF0938bzXvkTsu-6bsmXO6TNZs9IL92bVVvSMto7ugOyIkqN0tIfSXFc6gzW9JZRmd4kCbHHfhFDrFhU9q2pC3ppsHQKQa0W8imc2xzWp2VA6qzlJqmATEtLD68LYmVWzRZUnRGKWbNw12hT3CtlqK5TfWVLvLT5TyHOJiVc7r5cTSebj0kn65leB6RXpZn9jGhDhDCTMoFQIr5mumEBamIZQyUT0ZJ2id-BxVl2v7uaDNyqmqdJ7lq4KUAXqqGlzrvk9er3yya7iZXXr2DCFxdiZ3J6w_y4kS1iU4BJU2FYwakt2UWXg2AHsahTTQwZx6JPtnoEKnadFmqCzj2yaDD9MXX_36lJ1ff7QW5tXd0OFbj_cnBU3I7qCMLK4EbpFcVZ_YZuWl-AJyK520gU_LlutH-B0R2grI
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Nb9QwELVKQYgLHwLElgI-gNSKjXaTOHZyQIiyrKharVYIpN6M49glqJtskxS0_Un8BH4dM06yVZHorQeu-ZbzZvzGM55HyEujTGwEirsIiHQYxBBeHDDuJTaKufK1NYlyYhNiNouPjpL5Bvnd74XBssreJzpHnZUa18hHYQBcBfhCGI5sVxYxn0zfLk89VJDCTGsvp9FC5MCsfkL4Vr_Zn8C_fhUE0w-f33_0OoUBT8PE2XgpTN6ZHUeW21Rbno6jLNYiEkA7bKp8rrlSYx0oX6SZ5sCErAgTo5mvUwhzhArhuTfITYFNy13Z4LzHMmMihPBvjfUI44a4y9h9b5kV0hmnfefDAEVh1O_pifmoZiGYv4das0gifO_80rzp5AUuceK_0rhudpze-5_H9T6523Fy-q41ogdkwxQPya-JaVyZWkFLS_cgyGhKvQKHWFPcnZqbmuYFzXF7TYl1-VUJFmMy2jWqremORoOqhrRf3qYLbH7anNVDqoqM6rYtMa0MvrxLlNW7NF1R1Eup8vbltlLHuIJLUfKm-UaX5clqUYJ15PWC7nyaHs53H5Ev1zI8j8lmURbmCaEW0MJ0xgXAi_mKqZQFmUjiBIhgHKXZgPg9bKTuur6j-MiJdNFfzGULNQlQkw5q8nxAXq_vWbY9T668eg_RuL4S-5W7A2V1LDv3J4GoZsIyDQG5YQY-DUAfJqFJFfBpHokB2e7RKTsnWssLaA7IsMf3xel_f9LW1U97QW4DxOXh_uzgKbkTOCPD9OA22WyqM_OM3NI_AE3Vc2fRlHy9bqj_ATqAifE
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Detection+of+Blastocystis+species+in+immunocompromised+patients+%28cancer%2C+diabetes+mellitus%2C+and+chronic+renal+diseases%29+by+restriction+fragment+length+polymorphism+%28RFLP%29&rft.jtitle=Beni-Suef+University+journal+of+basic+and+applied+sciences&rft.au=Manal+Z.+M.+Abdellatif&rft.au=Ekhlas+H.+Abdel-Hafeez&rft.au=Usama+S.+Belal&rft.au=Noha+H.+Abdelgelil&rft.date=2025-04-30&rft.pub=SpringerOpen&rft.eissn=2314-8543&rft.volume=14&rft.issue=1&rft.spage=1&rft.epage=8&rft_id=info:doi/10.1186%2Fs43088-025-00631-z&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_074d7f4c014e4e098231393eba515657
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2314-8543&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2314-8543&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2314-8543&client=summon