Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP)

Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups...

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Published in:	Beni-Suef University journal of basic and applied sciences Vol. 14; no. 1; pp. 42 - 8
Main Authors:	Abdellatif, Manal Z. M., Abdel-Hafeez, Ekhlas H., Belal, Usama S., Abdelgelil, Noha H., Marey, Heba, Nasr, Manar M. S. A., Abdel-Fatah, Mohamed Mahmoud
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 30.04.2025 Springer Nature B.V SpringerOpen
Subjects:	Blastocystis Blastocystis spp Cancer Cysts Diabetes Diabetes mellitus Diarrhea Drinking water Enzymes Females Grouping Immuno-compromised Immunocompetence Immunocompromised hosts Infections Intestinal parasites Kidney diseases Medicine Medicine & Public Health Parasites Parasitic diseases Parasitology PCR–RFLP Polymerase chain reaction Polymorphism Protozoa Restriction fragment length polymorphism rRNA United States > US spp Immuno-compromised Grouping PCR–RFLP
ISSN:	2314-8543, 2314-8535, 2314-8543
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Abstract	Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations. Materials and methods A cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping. Results Among 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference ( P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases. Conclusion Blastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation.
AbstractList	BackgroundBlastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations.Materials and methodsA cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping.ResultsAmong 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference (P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases.ConclusionBlastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation. Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations. Materials and methods A cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping. Results Among 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference ( P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases. Conclusion Blastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation. Abstract Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans worldwide. Using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP), these subtypes are classified into four groups (A to D). This study aimed to detect Blastocystis spp. groups in immunocompromised patients and evaluate their correlation with clinical presentations. Materials and methods A cross-sectional study was conducted on 150 immunocompromised patients and 50 immunocompetent individuals. All subjects were evaluated for socio-demographic data, clinical features, and parasitic infections. RFLP analysis of the SSU rRNA gene was performed for Blastocystis spp. grouping. Results Among 150 immunocompromised patients, Blastocystis spp. was detected in 83.3% by direct wet mount and 94.7% by PCR, showing significant difference (P = 0.003) compared to immunocompetent individuals (40% and 50%, respectively). Of the PCR-positive immunocompromised patients, 86.7% were symptomatic, with diarrhea being the most common symptom (66%). In immunocompromised patients, group B of Blastocystis spp. subtypes (ST3, ST4, and ST8) dominated (86.7%). On the other hand, just 12% of immunocompetent people had symptoms, with group A subtypes (ST1 and ST2) accounting for 38% of the study cases. Conclusion Blastocystis spp. group B (ST3, ST4, and ST8) showed higher prevalence than group A (ST1 and ST2) in immunocompromised patients, with diarrhea being the most common clinical manifestation.
ArticleNumber	42
Author	Abdel-Hafeez, Ekhlas H. Abdellatif, Manal Z. M. Belal, Usama S. Marey, Heba Abdelgelil, Noha H. Nasr, Manar M. S. A. Abdel-Fatah, Mohamed Mahmoud
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Snippet	Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans... BackgroundBlastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from humans... Abstract Background Blastocystis spp. is an anaerobic protozoan intestinal parasite, it comprises multiple subtypes (STs), with ST1 to ST9 being isolated from...
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SubjectTerms	Blastocystis Blastocystis spp Cancer Cysts Diabetes Diabetes mellitus Diarrhea Drinking water Enzymes Females Grouping Immuno-compromised Immunocompetence Immunocompromised hosts Infections Intestinal parasites Kidney diseases Medicine Medicine & Public Health Parasites Parasitic diseases Parasitology PCR–RFLP Polymerase chain reaction Polymorphism Protozoa Restriction fragment length polymorphism rRNA
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Title	Detection of Blastocystis species in immunocompromised patients (cancer, diabetes mellitus, and chronic renal diseases) by restriction fragment length polymorphism (RFLP)
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