Comprehensive secondary-structure analysis of disulfide variants of lysozyme by synchrotron-radiation vacuum-ultraviolet circular dichroism

To elucidate the effects of specific disulfide bridges (Cys6‐Cys127, Cys30‐Cys115, Cys64‐Cys80, and Cys76‐Cys94) on the secondary structure of hen lysozyme, the vacuum‐ultraviolet circular dichroism (VUVCD) spectra of 13 species of disulfide‐deficient variants in which Cys residues were replaced wit...

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Published in:Proteins, structure, function, and bioinformatics Vol. 77; no. 1; pp. 191 - 201
Main Authors: Matsuo, Koichi, Watanabe, Hidenori, Tate, Shin-ichi, Tachibana, Hideki, Gekko, Kunihiko
Format: Journal Article
Language:English
Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.10.2009
Subjects:
Animals
Circular Dichroism - methods
disulfide variants
Disulfides - chemistry
Humans
lysozyme
Muramidase - chemistry
Protein Structure, Secondary
secondary structure
synchrotron radiation
Synchrotrons
vacuum-ultraviolet circular dichroism
ISSN:0887-3585, 1097-0134, 1097-0134
Online Access:Get full text
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Summary:To elucidate the effects of specific disulfide bridges (Cys6‐Cys127, Cys30‐Cys115, Cys64‐Cys80, and Cys76‐Cys94) on the secondary structure of hen lysozyme, the vacuum‐ultraviolet circular dichroism (VUVCD) spectra of 13 species of disulfide‐deficient variants in which Cys residues were replaced with Ala or Ser residues were measured down to 170 nm at pH 2.9 and 25°C using a synchrotron‐radiation VUVCD spectrophotometer. Each variant exhibited a VUVCD spectrum characteristic of a considerable amount of residual secondary structures depending on the positions and numbers of deleted disulfide bridges. The contents of α‐helices, β‐strands, turns, and unordered structures were estimated with the SELCON3 program using the VUVCD spectra and PDB data of 31 reference proteins. The numbers of α‐helix and β‐strand segments were also estimated from the VUVCD data. In general, the secondary structures were more effectively stabilized through entropic forces as the number of disulfide bridges increased and as they were formed over larger distances in the primary structure. The structures of three‐disulfide variants were similar to that of the wild type, but other variants exhibited diminished α‐helices with a border between the ordered and disordered structures around the two‐disulfide variants. The sequences of the secondary structures were predicted for all the variants by combining VUVCD data with a neural‐network method. These results revealed the characteristic role of each disulfide bridge in the formation of secondary structures. Proteins 2009. © 2009 Wiley‐Liss, Inc.
Bibliography:Ministry of Education, Science, Sports, and Culture of Japan - No. 20550153
istex:26E625D905B152FFE23182B48779572F25C4EA14
ark:/67375/WNG-N6B5X2C0-R
ArticleID:PROT22430
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0887-3585
1097-0134
1097-0134
DOI:10.1002/prot.22430