Vibrational spectroscopic methods for cytology and cellular research

The use of vibrational spectroscopy, FTIR and Raman, for cytology and cellular research has the potential to revolutionise the approach to cellular analysis. Vibrational spectroscopy is non-destructive, simple to operate and provides direct information. Importantly it does not require expensive exog...

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Bibliographic Details
Published in:Analyst (London) Vol. 139; no. 18; p. 4411
Main Authors: Clemens, Graeme, Hands, James R, Dorling, Konrad M, Baker, Matthew J
Format: Journal Article
Language:English
Published: England 21.09.2014
Subjects:
Animals
Cell Separation - instrumentation
Cell Separation - methods
Cytological Techniques - instrumentation
Cytological Techniques - methods
Equipment Design
Humans
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Spectroscopy, Fourier Transform Infrared - instrumentation
Spectroscopy, Fourier Transform Infrared - methods
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
ISSN:1364-5528, 1364-5528
Online Access:Get more information
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Summary:The use of vibrational spectroscopy, FTIR and Raman, for cytology and cellular research has the potential to revolutionise the approach to cellular analysis. Vibrational spectroscopy is non-destructive, simple to operate and provides direct information. Importantly it does not require expensive exogenous labels that may affect the chemistry of the cell under analysis. In addition, the advent of spectroscopic microscopes provides the ability to image cells and acquire spectra with a subcellular resolution. This introductory review focuses on recent developments within this fast paced field and highlights potential for the future use of FTIR and Raman spectroscopy. We particularly focus on the development of live cell research and the new technologies and methodologies that have enabled this.
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ISSN:1364-5528
1364-5528
DOI:10.1039/c4an00636d