A biophysical and structural analysis of the interaction of BLNK with 14-3-3 proteins

[Display omitted] •Phosphorylation of BLNK at Thr152 is important for 14-3-3 binding.•A 14-3-3σ/BLNKpT152 crystal structure confirms the binding mechanism.•BLNKpT152 binds to 14-3-3 proteins with micromolar affinity.•BLNK Ser285 has been confirmed to be an additional 14-3-3 binding site.•pThr152 and...

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Bibliographic Details
Published in:	Journal of structural biology Vol. 212; no. 3; p. 107662
Main Authors:	Soini, Lorenzo, Leysen, Seppe, Davis, Jeremy, Ottmann, Christian
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01.12.2020 Elsevier
Subjects:	Adaptor proteins 14-3-3 and BLNK Gatekeeper Isothermal titration calorimetry Life Sciences Phosphorylation X-ray protein crystallography Gatekeeper Phosphorylation ASU FL Adaptor proteins 14-3-3 and BLNK FP PDB PPIs X-ray protein crystallography BCR FITC IDR ITC Isothermal titration calorimetry
ISSN:	1047-8477, 1095-8657, 1095-8657
Online Access:	Get full text
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