Human cell‐derived microparticles promote thrombus formation in vivo in a tissue factor‐dependent manner

Background: Circulating microparticles of various cell types are present in healthy individuals and, in varying numbers and antigenic composition, in various disease states. To what extent these microparticles contribute to coagulation in vivo is unknown. Objectives: To examine the in vivo thromboge...

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Vydané v:Journal of thrombosis and haemostasis Ročník 1; číslo 12; s. 2561 - 2568
Hlavní autori: Biró, É., Sturk‐Maquelin, K. N., Vogel, G. M. T., Meuleman, D. G., Smit, M. J., Hack, C. E., Sturk, A., Nieuwland, R.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Oxford, UK Blackwell Science Inc 01.12.2003
Predmet:
Adult
Animals
Blood Coagulation
Blood Platelets
Case-Control Studies
Erythrocytes
experimental animal model
Female
Flow Cytometry
Granulocytes
Humans
Male
microparticles
Middle Aged
Particle Size
Pericardium
Rats
Thromboplastin - physiology
Thrombosis - blood
Thrombosis - etiology
thrombus
tissue factor
ISSN:1538-7933, 1538-7836, 1538-7836
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Shrnutí:Background: Circulating microparticles of various cell types are present in healthy individuals and, in varying numbers and antigenic composition, in various disease states. To what extent these microparticles contribute to coagulation in vivo is unknown. Objectives: To examine the in vivo thrombogenicity of human microparticles. Methods: Microparticles were isolated from pericardial blood of cardiac surgery patients and venous blood of healthy individuals. Their numbers, cellular source, and tissue factor (TF) exposure were determined using flow cytometry. Their in vitro procoagulant properties were studied in a fibrin generation test, and their in vivo thrombogenicity in a rat model. Results: The total number of microparticles did not differ between pericardial samples and samples from healthy individuals (P = 0.786). In both groups, microparticles from platelets, erythrocytes, and granulocytes exposed TF. Microparticle‐exposed TF antigen levels were higher in pericardial compared with healthy individual samples (P = 0.036). Pericardial microparticles were strongly procoagulant in vitro and highly thrombogenic in a venous stasis thrombosis model in rats, whereas microparticles from healthy individuals were not [thrombus weights 24.8 (12.2–41.3) mg vs. 0 (0–24.3) mg median and range; P < 0.001]. Preincubation of pericardial microparticles with an inhibitory antibody against human TF abolished their thrombogenicity [0 (0–4.4) mg; P < 0.01], while a control antibody had no effect [19.6 (12.6–53.7) mg; P > 0.05]. The thrombogenicity of the microparticles correlated strongly with their TF exposure (r = 0.9524, P = 0.001). Conclusions: Human cell‐derived microparticles promote thrombus formation in vivo in a TF‐dependent manner. They might be the direct cause of an increased thromboembolic tendency in various patient groups.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1538-7933
1538-7836
1538-7836
DOI:10.1046/j.1538-7836.2003.00456.x