Assessment of the GenoType MTBDRsl VER 2.0 compared to the phenotypic drug susceptibility testing and whole genome sequencing for the rapid detection of resistance to fluoroquinolone and second-line injectable drugs among rifampicin-resistant Mycobacterium tuberculosis isolates

Molecular techniques have considerable advantages for rapid detection, a reduction of infectiousness, prevention of further resistance development and surveillance of drug-resistant TB. MTBDR sl VER 2.0 was used to detect resistance to second-line anti-tuberculosis drugs on 35 rifampicin-resistant M...

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Veröffentlicht in:	Archives of microbiology Jg. 203; H. 7; S. 3989 - 3996
Hauptverfasser:	Kardan-Yamchi, Jalil, Amini, Sirus, Hamzelou, Gholamreza, Rahimi Foroushani, Abbas, Ghodousi, Arash, Cirillo, Daniela Maria, Feizabadi, Mohammad Mehdi
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2021 Springer Nature B.V
Schlagworte:	Antibiotics Biochemistry Biomedical and Life Sciences Biotechnology Capreomycin Cell Biology Drug resistance Drugs Ecology fluoroquinolones Gene sequencing genes Genomes genotype Genotypes Germany Kanamycin Life Sciences Microbial Ecology Microbiology minimum inhibitory concentration monitoring mutants Mutation Mycobacterium tuberculosis Original Paper phenotype rapid methods Rifampin Rrs gene Strains (organisms) Tuberculosis Whole genome sequencing Germany Resistance Mutation Drug susceptibility testing Whole genome sequencing MTBDR VER 2.0
ISSN:	0302-8933, 1432-072X, 1432-072X
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Abstract	Molecular techniques have considerable advantages for rapid detection, a reduction of infectiousness, prevention of further resistance development and surveillance of drug-resistant TB. MTBDR sl VER 2.0 was used to detect resistance to second-line anti-tuberculosis drugs on 35 rifampicin-resistant M. tuberculosis (RR-MTB) isolates compared to the minimum inhibitory concentrations (MICs) and whole genome sequencing (WGS). The MTBDR sl VER 2.0 (Hain Life Science, Nehren, Germany) and WGS (San Diego, CA, USA) were performed for tracing mutations in resistant-related genes involved in resistance to fluoroquinolone (FLQ) and second-line injectable drugs. The broth microdilution method using 7H9 Middlebrook media supplemented with OADC was used to determine the MICs. The MTBDR sl VER 2.0 correctly detected 5/6 (83.3%) of FLQ-resistant strains. The MUT1 A1401G (seven strains) and MUT2 G1484T (one strain) mutations in rrs gene were detected in eight AMK/KAN/CAP-resistant strains. Four low-level KAN-resistant strains with the G-10A/C-12T (three strains) and eis C-14T (one strain) mutations in eis gene was diagnosed using MTBDR sl VER 2.0. Five errors were found in detecting resistance to kanamycin and capreomycin compared to the phenotypic drug susceptibility testing and WGS. Failling wild-type bands without improved mutant bands did not indicate a reliable resistance. WGS could efficiently resolve the discrepancies of the results. MTBDR sl showed better performance in detecting XDR strains than pre-XDR.
AbstractList	Molecular techniques have considerable advantages for rapid detection, a reduction of infectiousness, prevention of further resistance development and surveillance of drug-resistant TB. MTBDR sl VER 2.0 was used to detect resistance to second-line anti-tuberculosis drugs on 35 rifampicin-resistant M. tuberculosis (RR-MTB) isolates compared to the minimum inhibitory concentrations (MICs) and whole genome sequencing (WGS). The MTBDR sl VER 2.0 (Hain Life Science, Nehren, Germany) and WGS (San Diego, CA, USA) were performed for tracing mutations in resistant-related genes involved in resistance to fluoroquinolone (FLQ) and second-line injectable drugs. The broth microdilution method using 7H9 Middlebrook media supplemented with OADC was used to determine the MICs. The MTBDR sl VER 2.0 correctly detected 5/6 (83.3%) of FLQ-resistant strains. The MUT1 A1401G (seven strains) and MUT2 G1484T (one strain) mutations in rrs gene were detected in eight AMK/KAN/CAP-resistant strains. Four low-level KAN-resistant strains with the G-10A/C-12T (three strains) and eis C-14T (one strain) mutations in eis gene was diagnosed using MTBDR sl VER 2.0. Five errors were found in detecting resistance to kanamycin and capreomycin compared to the phenotypic drug susceptibility testing and WGS. Failling wild-type bands without improved mutant bands did not indicate a reliable resistance. WGS could efficiently resolve the discrepancies of the results. MTBDR sl showed better performance in detecting XDR strains than pre-XDR. Molecular techniques have considerable advantages for rapid detection, a reduction of infectiousness, prevention of further resistance development and surveillance of drug-resistant TB. MTBDRsl VER 2.0 was used to detect resistance to second-line anti-tuberculosis drugs on 35 rifampicin-resistant M. tuberculosis (RR-MTB) isolates compared to the minimum inhibitory concentrations (MICs) and whole genome sequencing (WGS). The MTBDRsl VER 2.0 (Hain Life Science, Nehren, Germany) and WGS (San Diego, CA, USA) were performed for tracing mutations in resistant-related genes involved in resistance to fluoroquinolone (FLQ) and second-line injectable drugs. The broth microdilution method using 7H9 Middlebrook media supplemented with OADC was used to determine the MICs. The MTBDRsl VER 2.0 correctly detected 5/6 (83.3%) of FLQ-resistant strains. The MUT1 A1401G (seven strains) and MUT2 G1484T (one strain) mutations in rrs gene were detected in eight AMK/KAN/CAP-resistant strains. Four low-level KAN-resistant strains with the G-10A/C-12T (three strains) and eis C-14T (one strain) mutations in eis gene was diagnosed using MTBDRsl VER 2.0. Five errors were found in detecting resistance to kanamycin and capreomycin compared to the phenotypic drug susceptibility testing and WGS. Failling wild-type bands without improved mutant bands did not indicate a reliable resistance. WGS could efficiently resolve the discrepancies of the results. MTBDRsl showed better performance in detecting XDR strains than pre-XDR. Molecular techniques have considerable advantages for rapid detection, a reduction of infectiousness, prevention of further resistance development and surveillance of drug-resistant TB. MTBDRsl VER 2.0 was used to detect resistance to second-line anti-tuberculosis drugs on 35 rifampicin-resistant M. tuberculosis (RR-MTB) isolates compared to the minimum inhibitory concentrations (MICs) and whole genome sequencing (WGS). The MTBDRsl VER 2.0 (Hain Life Science, Nehren, Germany) and WGS (San Diego, CA, USA) were performed for tracing mutations in resistant-related genes involved in resistance to fluoroquinolone (FLQ) and second-line injectable drugs. The broth microdilution method using 7H9 Middlebrook media supplemented with OADC was used to determine the MICs. The MTBDRsl VER 2.0 correctly detected 5/6 (83.3%) of FLQ-resistant strains. The MUT1 A1401G (seven strains) and MUT2 G1484T (one strain) mutations in rrs gene were detected in eight AMK/KAN/CAP-resistant strains. Four low-level KAN-resistant strains with the G-10A/C-12T (three strains) and eis C-14T (one strain) mutations in eis gene was diagnosed using MTBDRsl VER 2.0. Five errors were found in detecting resistance to kanamycin and capreomycin compared to the phenotypic drug susceptibility testing and WGS. Failling wild-type bands without improved mutant bands did not indicate a reliable resistance. WGS could efficiently resolve the discrepancies of the results. MTBDRsl showed better performance in detecting XDR strains than pre-XDR.Molecular techniques have considerable advantages for rapid detection, a reduction of infectiousness, prevention of further resistance development and surveillance of drug-resistant TB. MTBDRsl VER 2.0 was used to detect resistance to second-line anti-tuberculosis drugs on 35 rifampicin-resistant M. tuberculosis (RR-MTB) isolates compared to the minimum inhibitory concentrations (MICs) and whole genome sequencing (WGS). The MTBDRsl VER 2.0 (Hain Life Science, Nehren, Germany) and WGS (San Diego, CA, USA) were performed for tracing mutations in resistant-related genes involved in resistance to fluoroquinolone (FLQ) and second-line injectable drugs. The broth microdilution method using 7H9 Middlebrook media supplemented with OADC was used to determine the MICs. The MTBDRsl VER 2.0 correctly detected 5/6 (83.3%) of FLQ-resistant strains. The MUT1 A1401G (seven strains) and MUT2 G1484T (one strain) mutations in rrs gene were detected in eight AMK/KAN/CAP-resistant strains. Four low-level KAN-resistant strains with the G-10A/C-12T (three strains) and eis C-14T (one strain) mutations in eis gene was diagnosed using MTBDRsl VER 2.0. Five errors were found in detecting resistance to kanamycin and capreomycin compared to the phenotypic drug susceptibility testing and WGS. Failling wild-type bands without improved mutant bands did not indicate a reliable resistance. WGS could efficiently resolve the discrepancies of the results. MTBDRsl showed better performance in detecting XDR strains than pre-XDR.
Author	Kardan-Yamchi, Jalil Rahimi Foroushani, Abbas Feizabadi, Mohammad Mehdi Amini, Sirus Cirillo, Daniela Maria Hamzelou, Gholamreza Ghodousi, Arash
Author_xml	– sequence: 1 givenname: Jalil surname: Kardan-Yamchi fullname: Kardan-Yamchi, Jalil organization: Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Department of Microbiology, School of Medicine, Tehran University of Medical Sciences – sequence: 2 givenname: Sirus surname: Amini fullname: Amini, Sirus organization: Tehran Regional Reference Laboratory for Tuberculosis, Tehran University of Medical Sciences – sequence: 3 givenname: Gholamreza surname: Hamzelou fullname: Hamzelou, Gholamreza organization: Tehran Regional Reference Laboratory for Tuberculosis, Tehran University of Medical Sciences – sequence: 4 givenname: Abbas surname: Rahimi Foroushani fullname: Rahimi Foroushani, Abbas organization: Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences – sequence: 5 givenname: Arash surname: Ghodousi fullname: Ghodousi, Arash organization: Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute – sequence: 6 givenname: Daniela Maria surname: Cirillo fullname: Cirillo, Daniela Maria organization: Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute – sequence: 7 givenname: Mohammad Mehdi orcidid: 0000-0002-4825-1170 surname: Feizabadi fullname: Feizabadi, Mohammad Mehdi email: mfeizabadi@tums.ac.ir organization: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Thoracic Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences
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CitedBy_id	crossref_primary_10_1038_s41598_023_35882_2 crossref_primary_10_1016_j_jgar_2023_05_001 crossref_primary_10_1016_j_arbres_2022_07_012 crossref_primary_10_1371_journal_pone_0294670 crossref_primary_10_1016_j_cca_2022_06_020 crossref_primary_10_1186_s12864_022_09042_1
Cites_doi	10.7717/peerj.5895 10.1586/ers.09.45 10.1128/AAC.00344-18 10.1128/JCM.01865-16 10.1186/1471-2334-13-240 10.1128/JCM.01257-15 10.1371/journal.pone.0033275 10.4103/0366-6999.208248 10.1128/JCM.00039-12 10.1371/journal.pone.0136861 10.1128/JCM.01138-16 10.1128/JCM.06664-11 10.1183/13993003.01354-2017 10.1016/j.diagmicrobio.2016.06.011 10.1128/AAC.49.2.571-577.2005 10.1073/pnas.0907925106 10.1016/j.tube.2014.11.003 10.1038/emi.2014.21 10.1128/JCM.00051-16
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Keywords	Resistance Mutation Drug susceptibility testing Whole genome sequencing MTBDR VER 2.0
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SubjectTerms	Antibiotics Biochemistry Biomedical and Life Sciences Biotechnology Capreomycin Cell Biology Drug resistance Drugs Ecology fluoroquinolones Gene sequencing genes Genomes genotype Genotypes Germany Kanamycin Life Sciences Microbial Ecology Microbiology minimum inhibitory concentration monitoring mutants Mutation Mycobacterium tuberculosis Original Paper phenotype rapid methods Rifampin Rrs gene Strains (organisms) Tuberculosis Whole genome sequencing
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Title	Assessment of the GenoType MTBDRsl VER 2.0 compared to the phenotypic drug susceptibility testing and whole genome sequencing for the rapid detection of resistance to fluoroquinolone and second-line injectable drugs among rifampicin-resistant Mycobacterium tuberculosis isolates
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