Three-dimensional nanofibrillar surfaces covalently modified with tenascin-C-derived peptides enhance neuronal growth in vitro

Current methods to promote growth of cultured neurons use two‐dimensional (2D) glass or polystyrene surfaces coated with a charged molecule (e.g. poly‐L‐lysine (PLL)) or an isolated extracellular matrix (ECM) protein (e.g. laminin‐1). However, these 2D surfaces represent a poor topological approxima...

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Published in:Journal of biomedical materials research. Part A Vol. 76A; no. 4; pp. 851 - 860
Main Authors: Ahmed, Ijaz, Liu, Hsing-Yin, Mamiya, Ping C., Ponery, Abdul S., Babu, Ashwin N., Weik, Thom, Schindler, Melvin, Meiners, Sally
Format: Journal Article
Language:English
Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.03.2006
Subjects:
Amino Acid Sequence
Animals
Biocompatible Materials
extracellular matrix
Molecular Sequence Data
nanofiber
Nanotechnology
neuron
Neurons - cytology
Neurons - drug effects
peptide
Peptides - chemistry
Peptides - pharmacology
Rats
Surface Properties
Tenascin - chemistry
Tenascin - pharmacology
tenascin-C
ISSN:1549-3296, 1552-4965
Online Access:Get full text
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Summary:Current methods to promote growth of cultured neurons use two‐dimensional (2D) glass or polystyrene surfaces coated with a charged molecule (e.g. poly‐L‐lysine (PLL)) or an isolated extracellular matrix (ECM) protein (e.g. laminin‐1). However, these 2D surfaces represent a poor topological approximation of the three‐dimensional (3D) architecture of the assembled ECM that regulates neuronal growth in vivo. Here we report on the development of a new 3D synthetic nanofibrillar surface for the culture of neurons. This nanofibrillar surface is composed of polyamide nanofibers whose organization mimics the porosity and geometry of the ECM. Neuronal adhesion and neurite outgrowth from cerebellar granule, cerebral cortical, hippocampal, motor, and dorsal root ganglion neurons were similar on nanofibers and PLL‐coated glass coverslips; however, neurite generation was increased. Moreover, covalent modification of the nanofibers with neuroactive peptides derived from human tenascin‐C significantly enhanced the ability of the nanofibers to facilitate neuronal attachment, neurite generation, and neurite extension in vitro. Hence the 3D nanofibrillar surface provides a physically and chemically stabile cell culture surface for neurons and, potentially, an exciting new opportunity for the development of peptide‐modified matrices for use in strategies designed to encourage axonal regrowth following central nervous system injury. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
Bibliography:istex:B63FD554E9F0BBF4DF7632DBA6F82A8302D6ECAC
New Jersey Commission on Spinal Cord Research - No. 04-3034 SCR-E-O
ark:/67375/WNG-77RMQ4X9-6
National Institutes of Health - No. R01 NS40394
ArticleID:JBM30587
One or more of the authors, has received or will receive remuneration or other perquisites for personal or professional use from a commercial or industrial agent in direct or indirect relationship to their authorship.
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ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.30587