Development and validation of a LC-MS/MS method for the establishment of reference intervals and biological variation for five plasma steroid hormones

With liquid chromatography–tandem mass spectrometry (LC-MS/MS) increasingly being used for the quantification of steroid hormones, there is a need for studies that re-establish reference intervals and biological variation in well-defined cohorts. A plasma steroid hormone profiling method using LC-MS...

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Published in:Clinical biochemistry Vol. 68; pp. 15 - 23
Main Authors: van der Veen, Anna, van Faassen, Martijn, de Jong, Wilhelmina H.A., van Beek, André P., Dijck-Brouwer, D.A. Janneke, Kema, Ido P.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01.06.2019
Subjects:
Biological variation
Dihydrotestosterone
LC-MS/MS
Reference intervals
Steroid hormones
LLOQ
RCV
SPE
CVa
P
LC-MS/MS
DHT
Dihydrotestosterone
Biological variation
T
CVg
ADION
CVi
QL
QN
II
17-OH-P
RIA
ACN
Reference intervals
PCOS
MeOH
FA
OCP
Steroid hormones
ISSN:0009-9120, 1873-2933, 1873-2933
Online Access:Get full text
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Summary:With liquid chromatography–tandem mass spectrometry (LC-MS/MS) increasingly being used for the quantification of steroid hormones, there is a need for studies that re-establish reference intervals and biological variation in well-defined cohorts. A plasma steroid hormone profiling method using LC-MS/MS for quantification of progesterone, 17-hydroxyprogesterone, androstenedione, testosterone and dihydrotestosterone was developed and validated. For reference interval assessment, 280 well-characterized healthy subjects from the LifeLines cohort were selected, including 40 women using oral contraceptive pills (OCP). The biological variation was examined in 30 healthy individuals. Samples were collected over a period of 4 months with 4 week intervals. The developed method proved to be robust and sensitive. The reference interval levels in men are higher, whereas in women the levels tend to decrease with increasing age. In addition, women using OCP had lower levels of 17-OH-progesterone and androstenedione. The biological variation is generally higher in women compared to men, especially with regard to the inter-individual variation. The gender-specific determination of the reference intervals, together with the observation that the biological variation demonstrated a high degree of variation, allows interpretation of data on individual and group level for improved biochemical characterization of patients in clinical practice. This figure was created using Servier Medical Art templates, which are licensed under a Creative Commons Attribution 3.0 License, and the photo of the LC-MS/MS is from https://waters.com. [Display omitted] •The LC-MS/MS method allows high-throughput analysis of 5 plasma steroid hormones.•The fully validated method allows the quantification of dihydrotestosterone.•The reference values were established in a well-defined healthy cohort.•The novel data on biological variation shows a high degree of individual variation.
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ISSN:0009-9120
1873-2933
1873-2933
DOI:10.1016/j.clinbiochem.2019.03.013