Glycerol-enhanced detection of a preferential structure latent in unstructured 1SS-variants of lysozyme

Four species of 1SS‐varinats of lysozyme were almost unstructured in water, judged from their near‐UV CD and 1H‐15N‐HSQC spectra. Some preferential structure might exist in such a disordered state, but the population of molecules in such a conformation must have been too small to be detected by spec...

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Veröffentlicht in:	Biopolymers Jg. 97; H. 7; S. 539 - 549
Hauptverfasser:	Noda, Yasuo, Narama, Kuniaki, Kasai, Kenichi, Tachibana, Hideki, Segawa, Shin-ichi
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.07.2012
Schlagworte:	1SS-variants of lysozyme chemical chaperones Circular Dichroism Glycerol - chemistry glycerol-enhanced detection Magnetic Resonance Spectroscopy Muramidase - chemistry NMR study of unstructured proteins Protein Conformation protein folding intermediates Spectrophotometry, Ultraviolet
ISSN:	0006-3525, 1097-0282
Online-Zugang:	Volltext
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