Dietary supplementation with l-arginine or N-carbamylglutamate enhances intestinal growth and heat shock protein-70 expression in weanling pigs fed a corn- and soybean meal-based diet

This study determined effects of dietary supplementation with l-arginine (Arg) or N-carbamylglutamate (NCG) on intestinal health and growth in early-weaned pigs. Eighty-four Landrace × Yorkshire pigs (average body weight of 5.56 ± 0.07 kg; weaned at 21 days of age) were fed for 7 days one of the thr...

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Vydáno v:	Amino acids Ročník 39; číslo 3; s. 831 - 839
Hlavní autoři:	Wu, Xin, Ruan, Zheng, Gao, Yunling, Yin, Yulong, Zhou, Xihong, Wang, Lei, Geng, Meimei, Hou, Yongqing, Wu, Guoyao
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Vienna Vienna : Springer Vienna 01.08.2010 Springer Vienna Springer Nature B.V
Témata:	analysis Analytical Chemistry Animal Feed Animal Feed - analysis Animals Arginine Arginine - metabolism Biochemical Engineering Biochemistry Biomedical and Life Sciences Body weight Body weight gain chemistry Corn Diarrhea Diet dietary supplements Dietary Supplements - analysis Duodenum Feed additives Female Gene Expression genetics Glutamates Glutamates - metabolism Glycine max - chemistry growth & development Growth performance Heat shock proteins heat stress Hogs HSP70 Heat-Shock Proteins HSP70 Heat-Shock Proteins - genetics HSP70 Heat-Shock Proteins - metabolism Hsp70 protein Ileum ingredients Intestinal morphology intestinal mucosa Intestine Intestine, Small Intestine, Small - growth & development Intestine, Small - metabolism Jejunum l-Arginine landraces Life Sciences Male messenger RNA metabolism N-carbamylglutamate Neonates Neurobiology Nutrients Original Article physiology piglets Polymerase chain reaction Proteomics quantitative polymerase chain reaction soybeans Sus scrofa Sus scrofa - genetics Sus scrofa - growth & development Sus scrofa - physiology swine feeding villi Villus Weaned pigs Weaning weanlings weight gain Western blotting Zea mays Zea mays - chemistry Diarrhea Weaned pigs carbamylglutamate Arginine Growth performance Intestinal morphology
ISSN:	0939-4451, 1438-2199, 1438-2199
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Shrnutí:	This study determined effects of dietary supplementation with l-arginine (Arg) or N-carbamylglutamate (NCG) on intestinal health and growth in early-weaned pigs. Eighty-four Landrace × Yorkshire pigs (average body weight of 5.56 ± 0.07 kg; weaned at 21 days of age) were fed for 7 days one of the three isonitrogenous diets: (1) a corn- and soybean meal-based diet (CSM), (2) CSM + 0.08% NCG (0.08%), and (3) CSM + 0.6% Arg. There were four pens of pigs per diet (7 pigs/pen). At the end of a 7-day feeding period, six piglets were randomly selected from each treatment for tissue collections. Compared with the control group, Arg or NCG supplementation increased (P < 0.05): (1) Arg concentrations in plasma, (2) small-intestinal growth, (3) villus height in duodenum, jejunum and ileum, (4) crypt depth in jejunum and ileum, (5) goblet cell counts in intestinal mucosae, and (6) whole-body weight gain in pigs. Real-time polymerase chain reaction and western blotting analyses revealed that both mRNA and protein levels for heat shock protein-70 (HSP70) were higher (P < 0.05) in the intestinal mucosae of Arg- or NCG-supplemented pigs than in the control group. Furthermore, the incidence of diarrhea in the NCG group was 18% lower (P < 0.01) than that in the control group. Collectively, these results indicate that dietary supplementation with 0.6% Arg or 0.08% NCG enhances intestinal HSP70 gene expression, intestinal growth and integrity, and the availability of dietary nutrients for whole-body weight gain in postweaning pigs fed a CSM-based diet. Thus, Arg or NCG is a functional ingredient in the weaning diet to improve nutrition, health, and growth performance of these neonates.
Bibliografie:	http://dx.doi.org/10.1007/s00726-010-0538-y ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0939-4451 1438-2199 1438-2199
DOI:	10.1007/s00726-010-0538-y