Ion selectivity of scorpion toxin-induced pores in cardiac myocytes

The lytic activity of parabutoporin (PP) and opistoporin 1 (OP1) on mammalian and bacterial membranes have been described. We investigated pore-formation and ion selectivity in cardiac myocytes by measuring the whole cell leak current by means of the patch clamp technique. Pore formation was observe...

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Veröffentlicht in:Peptides (New York, N.Y. : 1980) Jg. 27; H. 1; S. 55 - 61
Hauptverfasser: Elgar, Dale, Verdonck, Fons, Grobler, Anne, Fourie, Carla, du Plessis, Johan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: New York, NY Elsevier Inc 2006
Elsevier Science
Schlagworte:
Animals
Antimicrobial
Antimicrobial Cationic Peptides - toxicity
Biological and medical sciences
Cations, Divalent - metabolism
Cations, Monovalent - metabolism
Cell Membrane - drug effects
Cell Membrane - metabolism
Cells, Cultured
Fundamental and applied biological sciences. Psychology
Gramicidin - pharmacology
Ion selectivity
Membrane Potentials - drug effects
Membrane Potentials - physiology
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - metabolism
Peptides
Pore-forming
Rats
Rats, Sprague-Dawley
Scorpion
Scorpion Venoms - toxicity
Scorpions - physiology
Venom
Vertebrates: endocrinology
Antimicrobial
Peptides
Scorpion
Pore-forming
Venom
Ion selectivity
Heart
Toxin
Pore-forming Peptides Antimicrobial Ion selectivity Scorpion Venom
Ions
Selectivity
Circulatory system
Antibacterial agent
Antimicrobial agent
Myocyte
ISSN:0196-9781, 1873-5169
Online-Zugang:Volltext
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Zusammenfassung:The lytic activity of parabutoporin (PP) and opistoporin 1 (OP1) on mammalian and bacterial membranes have been described. We investigated pore-formation and ion selectivity in cardiac myocytes by measuring the whole cell leak current by means of the patch clamp technique. Pore formation was observed as the induction of leak currents. Ion selectivity of the pores was indicated by the shift of the reversal potential ( E rev) upon substitution of intra- and extra-cellular ions. Results were compared with the effect of gramicidin A (gramA). PP and OP1 induced a fluctuating leak current and indicate non-selectivity of PP-induced pores. PP- and OP1-induced pores are between 1.38 and 1.78 nm in diameter.
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ISSN:0196-9781
1873-5169
DOI:10.1016/j.peptides.2005.06.018