Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes
Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be...
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| Vydáno v: | Journal of virological methods Ročník 142; číslo 1; s. 218 - 222 |
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| Médium: | Journal Article |
| Jazyk: | angličtina |
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London
Elsevier B.V
01.06.2007
Amsterdam Elsevier New York, NY |
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| ISSN: | 0166-0934, 1879-0984 |
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| Abstract | Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes. |
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| AbstractList | Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes.Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes. Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes. |
| Author | Leung, Connie Y.H. Barr, Ian Peiris, J.S. Malik Poon, Leo L.M. Guan, Yi Li, Olive T.W. Chen, Honglin |
| Author_xml | – sequence: 1 givenname: Olive T.W. surname: Li fullname: Li, Olive T.W. organization: State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China – sequence: 2 givenname: Ian surname: Barr fullname: Barr, Ian organization: WHO Collaborating Centre for Influenza, Melbourne, Australia – sequence: 3 givenname: Connie Y.H. surname: Leung fullname: Leung, Connie Y.H. organization: State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China – sequence: 4 givenname: Honglin surname: Chen fullname: Chen, Honglin organization: State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China – sequence: 5 givenname: Yi surname: Guan fullname: Guan, Yi organization: State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China – sequence: 6 givenname: J.S. Malik surname: Peiris fullname: Peiris, J.S. Malik organization: State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China – sequence: 7 givenname: Leo L.M. surname: Poon fullname: Poon, Leo L.M. email: llmpoon@hkucc.hku.hk organization: State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China |
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| Cites_doi | 10.1002/bies.10303 10.1016/j.jcv.2003.08.004 10.1016/j.virol.2003.11.030 10.1128/JVI.79.5.2814-2822.2005 10.1128/JVI.64.10.4893-4902.1990 10.1006/viro.1994.1067 10.1016/j.virol.2006.03.048 10.1016/j.jviromet.2006.03.027 10.1084/jem.20051938 10.1128/JCM.42.9.4349-4354.2004 10.1016/0042-6822(89)90202-X 10.1007/s007050170002 10.1038/442037a 10.1038/nature04239 10.1101/gr.849004 10.1038/nature03974 |
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| Keywords | Polymerase chain reaction Polymerase genes Influenza virus Consensus primers Microbiology Nucleotide sequence Hemagglutinin Orthomyxoviridae Method Subunit Influenzavirus Virology Infection Virus Gene Viral disease Influenza Reverse transcription polymerase chain reaction Subtype |
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| SubjectTerms | Animals Base Sequence Biological and medical sciences Consensus primers Consensus Sequence DNA Primers Fundamental and applied biological sciences. Psychology Hemagglutinin Glycoproteins, Influenza Virus - classification Hemagglutinin Glycoproteins, Influenza Virus - genetics Humans Influenza A virus - classification Influenza A virus - enzymology Influenza A virus - genetics Influenza virus Microbiology Molecular Sequence Data Polymerase chain reaction Polymerase Chain Reaction - methods Polymerase genes Reverse Transcriptase Polymerase Chain Reaction - methods RNA Replicase - chemistry RNA Replicase - genetics Techniques used in virology Viral Proteins - chemistry Viral Proteins - genetics Virology |
| Title | Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes |
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