Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes

Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be...

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Vydané v:	Journal of virological methods Ročník 142; číslo 1; s. 218 - 222
Hlavní autori:	Li, Olive T.W., Barr, Ian, Leung, Connie Y.H., Chen, Honglin, Guan, Yi, Peiris, J.S. Malik, Poon, Leo L.M.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London Elsevier B.V 01.06.2007 Amsterdam Elsevier New York, NY
Predmet:	Animals Base Sequence Biological and medical sciences Consensus primers Consensus Sequence DNA Primers Fundamental and applied biological sciences. Psychology Hemagglutinin Glycoproteins, Influenza Virus - classification Hemagglutinin Glycoproteins, Influenza Virus - genetics Humans Influenza A virus - classification Influenza A virus - enzymology Influenza A virus - genetics Influenza virus Microbiology Molecular Sequence Data Polymerase chain reaction Polymerase Chain Reaction - methods Polymerase genes Reverse Transcriptase Polymerase Chain Reaction - methods RNA Replicase - chemistry RNA Replicase - genetics Techniques used in virology Viral Proteins - chemistry Viral Proteins - genetics Virology Polymerase chain reaction Polymerase genes Influenza virus Consensus primers Microbiology Nucleotide sequence Hemagglutinin Orthomyxoviridae Method Subunit Influenzavirus Virology Infection Virus Gene Viral disease Influenza Reverse transcription polymerase chain reaction Subtype
ISSN:	0166-0934, 1879-0984
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Shrnutí:	Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Undefined-1 ObjectType-Feature-3
ISSN:	0166-0934 1879-0984
DOI:	10.1016/j.jviromet.2007.01.015