Vital marking of articular chondrocytes by retroviral infection using green fluorescence protein
Objective One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such studies in animal models and to show the feasibility of this approach in rabbits. Isolated articular chondrocytes were retrovirally mark...
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| Published in: | Osteoarthritis and cartilage Vol. 10; no. 2; pp. 109 - 118 |
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| Format: | Journal Article |
| Language: | English |
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Elsevier Ltd
01.02.2002
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| ISSN: | 1063-4584, 1522-9653 |
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| Abstract | Objective One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such studies in animal models and to show the feasibility of this approach in rabbits. Isolated articular chondrocytes were retrovirally marked using green fluorescence protein (GFP) as a cell-specific marker in order to allow an in vivo follow-up of these cells.
Methods Chondrocytes from rabbits, sheep, cattle and humans were isolated and infected with murine leukemia virus-derived retroviruses carrying the GFP gene. The influence of the host range of three packaging cell lines (PA317, PT67, PG13), start cell concentrations, number of cell passages and number of infection cycles on the efficiency of infection was investigated. Stability of GFP expression was followed by FACS analysis, confocal imaging and fluorescence microscopy. For in vivo follow-up of GFP expression we used marked allogeneic chondrocyte populations grown on scaffold material and implanted them into full-thickness defects in knee joints of rabbits.
ResultsRetroviruses from all three packaging cell lines were able to infect rabbit and human chondrocytes, whereas only retroviruses released from PG13 cells were able to infect sheep and bovine chondrocytes efficiently. Optimization of the infection with these viruses resulted in efficiencies of 60–90% GFP-expressing chondrocytes. Populations of 100% marked chondrocytes were obtained by cell sorting. GFP expression stability of such marked chondrocyte populations was followed in monolayer culture and in 3-D culture on different scaffold materials. The expression of GFP was stable on all tested materials for at least 4 weeks. In monolayer culture GFP expression was stable for more than 8 months. In vivo, we observed stable GFP expression in the transplants during a four-week time course.
Conclusion Retroviral GFP gene transfer led to long-term expression in chondrocytes from rabbits, sheep, cattle and humans. Transgene expression and the number of implanted chondrocytes remain stable for at least 4 weeks in vivo. This method permits a rapid monitoring of chondrocytes and provides a basis for following the fate of these cells in vivo. |
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| AbstractList | One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such studies in animal models and to show the feasibility of this approach in rabbits. Isolated articular chondrocytes were retrovirally marked using green fluorescence protein (GFP) as a cell-specific marker in order to allow an in vivo follow-up of these cells.OBJECTIVEOne of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such studies in animal models and to show the feasibility of this approach in rabbits. Isolated articular chondrocytes were retrovirally marked using green fluorescence protein (GFP) as a cell-specific marker in order to allow an in vivo follow-up of these cells.Chondrocytes from rabbits, sheep, cattle and humans were isolated and infected with murine leukemia virus-derived retroviruses carrying the GFP gene. The influence of the host range of three packaging cell lines (PA317, PT67, PG13), start cell concentrations, number of cell passages and number of infection cycles on the efficiency of infection was investigated. Stability of GFP expression was followed by FACS analysis, confocal imaging and fluorescence microscopy. For in vivo follow-up of GFP expression we used marked allogeneic chondrocyte populations grown on scaffold material and implanted them into full-thickness defects in knee joints of rabbits.METHODSChondrocytes from rabbits, sheep, cattle and humans were isolated and infected with murine leukemia virus-derived retroviruses carrying the GFP gene. The influence of the host range of three packaging cell lines (PA317, PT67, PG13), start cell concentrations, number of cell passages and number of infection cycles on the efficiency of infection was investigated. Stability of GFP expression was followed by FACS analysis, confocal imaging and fluorescence microscopy. For in vivo follow-up of GFP expression we used marked allogeneic chondrocyte populations grown on scaffold material and implanted them into full-thickness defects in knee joints of rabbits.Retroviruses from all three packaging cell lines were able to infect rabbit and human chondrocytes, whereas only retroviruses released from PG13 cells were able to infect sheep and bovine chondrocytes efficiently. Optimization of the infection with these viruses resulted in efficiencies of 60-90% GFP-expressing chondrocytes. Populations of 100% marked chondrocytes were obtained by cell sorting. GFP expression stability of such marked chondrocyte populations was followed in monolayer culture and in 3-D culture on different scaffold materials. The expression of GFP was stable on all tested materials for at least 4 weeks. In monolayer culture GFP expression was stable for more than 8 months. In vivo, we observed stable GFP expression in the transplants during a four-week time course.RESULTSRetroviruses from all three packaging cell lines were able to infect rabbit and human chondrocytes, whereas only retroviruses released from PG13 cells were able to infect sheep and bovine chondrocytes efficiently. Optimization of the infection with these viruses resulted in efficiencies of 60-90% GFP-expressing chondrocytes. Populations of 100% marked chondrocytes were obtained by cell sorting. GFP expression stability of such marked chondrocyte populations was followed in monolayer culture and in 3-D culture on different scaffold materials. The expression of GFP was stable on all tested materials for at least 4 weeks. In monolayer culture GFP expression was stable for more than 8 months. In vivo, we observed stable GFP expression in the transplants during a four-week time course.Retroviral GFP gene transfer led to long-term expression in chondrocytes from rabbits, sheep, cattle and humans. Transgene expression and the number of implanted chondrocytes remain stable for at least 4 weeks in vivo. This method permits a rapid monitoring of chondrocytes and provides a basis for following the fate of these cells in vivo.CONCLUSIONRetroviral GFP gene transfer led to long-term expression in chondrocytes from rabbits, sheep, cattle and humans. Transgene expression and the number of implanted chondrocytes remain stable for at least 4 weeks in vivo. This method permits a rapid monitoring of chondrocytes and provides a basis for following the fate of these cells in vivo. Objective One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such studies in animal models and to show the feasibility of this approach in rabbits. Isolated articular chondrocytes were retrovirally marked using green fluorescence protein (GFP) as a cell-specific marker in order to allow an in vivo follow-up of these cells. Methods Chondrocytes from rabbits, sheep, cattle and humans were isolated and infected with murine leukemia virus-derived retroviruses carrying the GFP gene. The influence of the host range of three packaging cell lines (PA317, PT67, PG13), start cell concentrations, number of cell passages and number of infection cycles on the efficiency of infection was investigated. Stability of GFP expression was followed by FACS analysis, confocal imaging and fluorescence microscopy. For in vivo follow-up of GFP expression we used marked allogeneic chondrocyte populations grown on scaffold material and implanted them into full-thickness defects in knee joints of rabbits. ResultsRetroviruses from all three packaging cell lines were able to infect rabbit and human chondrocytes, whereas only retroviruses released from PG13 cells were able to infect sheep and bovine chondrocytes efficiently. Optimization of the infection with these viruses resulted in efficiencies of 60–90% GFP-expressing chondrocytes. Populations of 100% marked chondrocytes were obtained by cell sorting. GFP expression stability of such marked chondrocyte populations was followed in monolayer culture and in 3-D culture on different scaffold materials. The expression of GFP was stable on all tested materials for at least 4 weeks. In monolayer culture GFP expression was stable for more than 8 months. In vivo, we observed stable GFP expression in the transplants during a four-week time course. Conclusion Retroviral GFP gene transfer led to long-term expression in chondrocytes from rabbits, sheep, cattle and humans. Transgene expression and the number of implanted chondrocytes remain stable for at least 4 weeks in vivo. This method permits a rapid monitoring of chondrocytes and provides a basis for following the fate of these cells in vivo. One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such studies in animal models and to show the feasibility of this approach in rabbits. Isolated articular chondrocytes were retrovirally marked using green fluorescence protein (GFP) as a cell-specific marker in order to allow an in vivo follow-up of these cells. Chondrocytes from rabbits, sheep, cattle and humans were isolated and infected with murine leukemia virus-derived retroviruses carrying the GFP gene. The influence of the host range of three packaging cell lines (PA317, PT67, PG13), start cell concentrations, number of cell passages and number of infection cycles on the efficiency of infection was investigated. Stability of GFP expression was followed by FACS analysis, confocal imaging and fluorescence microscopy. For in vivo follow-up of GFP expression we used marked allogeneic chondrocyte populations grown on scaffold material and implanted them into full-thickness defects in knee joints of rabbits. Retroviruses from all three packaging cell lines were able to infect rabbit and human chondrocytes, whereas only retroviruses released from PG13 cells were able to infect sheep and bovine chondrocytes efficiently. Optimization of the infection with these viruses resulted in efficiencies of 60-90% GFP-expressing chondrocytes. Populations of 100% marked chondrocytes were obtained by cell sorting. GFP expression stability of such marked chondrocyte populations was followed in monolayer culture and in 3-D culture on different scaffold materials. The expression of GFP was stable on all tested materials for at least 4 weeks. In monolayer culture GFP expression was stable for more than 8 months. In vivo, we observed stable GFP expression in the transplants during a four-week time course. Retroviral GFP gene transfer led to long-term expression in chondrocytes from rabbits, sheep, cattle and humans. Transgene expression and the number of implanted chondrocytes remain stable for at least 4 weeks in vivo. This method permits a rapid monitoring of chondrocytes and provides a basis for following the fate of these cells in vivo. |
| Author | Bauwens, S. Wirth, D. Hirschmann, F. Verhoeyen, E. Rudert, M. Hauser, H. |
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| Cites_doi | 10.2106/00004623-199710000-00001 10.1182/blood.V91.2.431 10.1053/joca.1998.0107 10.1007/s001320050237 10.1016/S0076-6879(99)02023-6 10.1093/protein/12.12.1035 10.1038/sj.gt.3300654 10.1002/1529-0131(199808)41:8<1331::AID-ART2>3.0.CO;2-J 10.1002/jor.1100160208 10.1292/jvms.61.843 10.1089/10430349950017572 10.1099/0022-1317-80-12-3049 10.1172/JCI118303 10.1016/S1063-4584(97)80023-4 10.1016/S0940-9602(99)80055-7 10.1006/excr.1999.4478 10.3109/17453679608996682 10.1053/joca.1997.0092 10.1002/jbm.820280808 10.1159/000016773 10.1002/(SICI)1097-4652(199808)176:2<303::AID-JCP8>3.0.CO;2-S 10.1128/jvi.70.8.5564-5571.1996 10.1038/nbt0597-458 10.1128/jvi.71.10.7533-7540.1997 10.1016/S1063-4584(97)80007-6 10.1056/NEJM199410063311401 10.1016/S0076-6879(99)02032-7 10.1006/excr.1998.4010 10.1038/sj.gt.3301086 10.1002/(SICI)1097-4636(19981205)42:3<347::AID-JBM2>3.0.CO;2-J 10.1146/annurev.biochem.67.1.509 10.1016/0378-1119(91)90134-W |
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| Copyright | 2002 OsteoArthritis Research Society International Copyright 2002 OsteoArthritis Research Society International. |
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| Keywords | Articular chondrocytes, Retrovirus, GFP marking, Cell transplantation |
| Language | English |
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| References | Corish, Tyler-Smith (RF15) 1999; 12 Rotter, Aigner, Naumann, Planck, Hammer, Burmester (RF32) 1998; 42 Artelt, Grannemann, Stocking, Friel, Bartsch, Hauser (RF36) 1991; 99 Rudert, Hirschmann, Wirth (RF31) 1999; 28 Sommerfelt (RF27) 1999; 80 Ehlers, Fuss, Rohwedel, Russlies, Kühnel, Behrens (RF26) 1999; 181 Fernex, Dubreuil, Mannoni, Bagnis (RF37) 1997; 71 Wildner, Condotti, Krecko, Xanthopoulos, Ramsey, Blaese (RF38) 1998; 5 Freed, Grande, Lingbin, Emmanual, Marquis, Langer (RF4) 1994; 28 Chu (RF34) 1995 Ikeda, Kubo, Arai, Nakanishi, Kobayashi, Takahashi (RF23) 1998; 25 Levy, Muldoon, Mazo, Kain, Link (RF18) 1999; 302 Spitzer, Hauser, Wirth (RF30) 1999; 10 Kang, Marui, Ghivizzani, Nita, Georgescu, Suh (RF10) 1997; 5 Binette, McQuaid, Haudenschild, Yaeger, McPherson, Tubo (RF8) 1998; 16 Freed, Hollander, Martin, Barry, Langer, Vunjak-Novakovic (RF9) 1998; 240 Brittberg, Lindahl, Nilsson, Ohlsson, Isaksson, Peterson (RF3) 1994; 331 Madry, Trippel (RF20) 2000; 7 Li (RF33) 1995 Robbins, Goldring (RF24) 1999 Lemare, Steimberg, Le Griel, Demignot, Adolphe (RF7) 1998; 176 Tsien (RF14) 1998; 67 Breinan, Minas, Hsu, Nehrer, Sledge, Spector (RF5) 1997; 79 Takada, Iida, Awaji, Itoh, Takahashi, Shibui (RF16) 1997; 15 Murakami, Fahrudin, Varisanga, Suzuki (RF19) 1999; 61 Baragi, Renkiewicz, Qiu, Brammer, Riley, Sigler (RF11) 1997; 5 Rahfoth, Weisser, Sternkopf, Aigner, von-der-Mark, Brauer (RF25) 1998; 6 Verhasselt, De Smedt, Verhelst, Naessens, Plum (RF29) 1998; 91 Steimberg, Viengchareun, Biehlmann, Guenal, Mignotte, Adolphe (RF21) 1999; 249 Baragi, Renkiewicz, Jordan, Bonadio, Hartman, Roessler (RF22) 1995; 96 Doherty, Zhang, Tremblay, Manolopoulos, Marshall (RF12) 1998; 6 Messner, Gillquist (RF6) 1996; 67 Buckwalter, Mankin (RF1) 1998; 41 Takada, Yoshida, Nakamura, Nakao, Tsujimoto, Katsuki (RF17) 1999; 302 Miller, Chen (RF28) 1996; 70 Goater, Müller, Kollias, Firestein, Sanz, O'Keefe (RF13) 2000; 27 Rudert, Wirth (RF2) 1998; 27 Rudert, Hirschmann, Schulze, Wirth (RF35) 2000; 167 Sommerfelt (10.1053/joca.2001.0486_RF27) 1999; 80 Miller (10.1053/joca.2001.0486_RF28) 1996; 70 Takada (10.1053/joca.2001.0486_RF17) 1999; 302 Spitzer (10.1053/joca.2001.0486_RF30) 1999; 10 Levy (10.1053/joca.2001.0486_RF18) 1999; 302 Chu (10.1053/joca.2001.0486_RF34) 1995 Kang (10.1053/joca.2001.0486_RF10) 1997; 5 Wildner (10.1053/joca.2001.0486_RF38) 1998; 5 Steimberg (10.1053/joca.2001.0486_RF21) 1999; 249 Baragi (10.1053/joca.2001.0486_RF11) 1997; 5 Li (10.1053/joca.2001.0486_RF33) 1995 Messner (10.1053/joca.2001.0486_RF6) 1996; 67 Fernex (10.1053/joca.2001.0486_RF37) 1997; 71 Rudert (10.1053/joca.2001.0486_RF2) 1998; 27 Freed (10.1053/joca.2001.0486_RF9) 1998; 240 Goater (10.1053/joca.2001.0486_RF13) 2000; 27 Takada (10.1053/joca.2001.0486_RF16) 1997; 15 Madry (10.1053/joca.2001.0486_RF20) 2000; 7 Binette (10.1053/joca.2001.0486_RF8) 1998; 16 Verhasselt (10.1053/joca.2001.0486_RF29) 1998; 91 Ikeda (10.1053/joca.2001.0486_RF23) 1998; 25 Artelt (10.1053/joca.2001.0486_RF36) 1991; 99 Freed (10.1053/joca.2001.0486_RF4) 1994; 28 Lemare (10.1053/joca.2001.0486_RF7) 1998; 176 Brittberg (10.1053/joca.2001.0486_RF3) 1994; 331 Baragi (10.1053/joca.2001.0486_RF22) 1995; 96 Doherty (10.1053/joca.2001.0486_RF12) 1998; 6 Rudert (10.1053/joca.2001.0486_RF35) 2000; 167 Corish (10.1053/joca.2001.0486_RF15) 1999; 12 Rotter (10.1053/joca.2001.0486_RF32) 1998; 42 Murakami (10.1053/joca.2001.0486_RF19) 1999; 61 Tsien (10.1053/joca.2001.0486_RF14) 1998; 67 Rahfoth (10.1053/joca.2001.0486_RF25) 1998; 6 Breinan (10.1053/joca.2001.0486_RF5) 1997; 79 Rudert (10.1053/joca.2001.0486_RF31) 1999; 28 Buckwalter (10.1053/joca.2001.0486_RF1) 1998; 41 Robbins (10.1053/joca.2001.0486_RF24) 1999 Ehlers (10.1053/joca.2001.0486_RF26) 1999; 181 |
| References_xml | – volume: 15 start-page: 458 year: 1997 end-page: 461 ident: RF16 article-title: Selective production of transgenic mice using green fluorescent protein as a marker publication-title: Nature Biotech – volume: 5 start-page: 139, 43 year: 1997 ident: RF10 article-title: Ex vivo gene transfer to chondrocytes in full-thickness articular cartilage defects: a feasibility study publication-title: Osteoarthritis Cart – volume: 10 start-page: 1893 year: 1999 end-page: 1902 ident: RF30 article-title: Complement-protected amphotropic retroviruses from murine packaging cells publication-title: Human Gene Therapy – volume: 5 start-page: 275 year: 1997 end-page: 282 ident: RF11 article-title: Transplantation of adenovirally transduced allogeneic chondrocytes into articular cartilage defects publication-title: Osteoarthritis Cart – volume: 181 start-page: 513 year: 1999 end-page: 518 ident: RF26 article-title: Development of a biocomposite to fill out articular cartilage lesions. Light, scanning and transmission electron microscopy of sheep chondrocytes cultured on a collagen I/III sponge publication-title: Anatomischer Anzeiger – volume: 12 start-page: 1035 year: 1999 end-page: 1040 ident: RF15 article-title: Attenuation of green fluorescent protein half-line in mammalian cells publication-title: Protein Engineering – volume: 96 start-page: 2454 year: 1995 end-page: 2460 ident: RF22 article-title: Transplantation of transduced chondrocytes protects articular cartilage from interleukin 1-induced extracellular matrix degradation publication-title: J Clin Invest – volume: 79 start-page: 1439 year: 1997 end-page: 1451 ident: RF5 article-title: Effect of cultured autologous chondrocytes on repair of chondral defects in a canine model publication-title: J Bone and Joint Surg – volume: 16 start-page: 207 year: 1998 end-page: 216 ident: RF8 article-title: Expression of a stable articular cartilage phenotype without evidence of hypertrophy by adult human articular chondrocytes publication-title: J Orthopaedic Res – volume: 99 start-page: 249 year: 1991 end-page: 254 ident: RF36 article-title: The prokaryotic neomycin-resistance-encoding gene acts as a transcriptional silencer in eukaryotic cells publication-title: Gene – volume: 71 start-page: 7533 year: 1997 end-page: 7540 ident: RF37 article-title: Cre/loxP-mediated excision of a neomycin resistance expression unit from an integrated retroviral vector increases long terminal repeat-driven transcription in human hematopoietic cells publication-title: J Virol – volume: 302 start-page: 358 year: 1999 end-page: 369 ident: RF18 article-title: In vivo retroviral transduction and expression of green fluorescent protein publication-title: Methods in Enzymology – volume: 61 start-page: 843 year: 1999 end-page: 847 ident: RF19 article-title: Fluorescence expression by bovine embryos after pronuclear microinjection with the EGFP gene publication-title: J Veterinary Med Sci – volume: 167 start-page: 95 year: 2000 end-page: 105 ident: RF35 article-title: Bioartificial cartilage publication-title: Cells Tissues Organs – start-page: 611 year: 1995 end-page: 626 ident: RF34 article-title: Biodegradable polymeric biomaterials: An overview publication-title: The Biomedical Engineering Handbook – volume: 42 start-page: 347 year: 1998 end-page: 356 ident: RF32 article-title: Cartilage reconstruction in head and neck surgery: comparison of resorbable polymer scaffolds for tissue engineering of human septal cartilage publication-title: J Biomed Mat Res – volume: 28 start-page: 68 year: 1999 end-page: 75 ident: RF31 article-title: Wachstumsverhalten von Chondrozyten auf unterschiedlichen Trägersubstanzen publication-title: Orthopäde – volume: 67 start-page: 509 year: 1998 end-page: 544 ident: RF14 article-title: The green fluorescent protein publication-title: Ann Rev Biochem – volume: 91 start-page: 431 year: 1998 end-page: 440 ident: RF29 article-title: Retrovirally transduced CD34 publication-title: Blood – volume: 5 start-page: 684 year: 1998 end-page: 691 ident: RF38 article-title: Generation of a conditionally publication-title: Gene Therapy – volume: 27 start-page: 309 year: 1998 end-page: 321 ident: RF2 article-title: Knorpelregeneration und Knorpelersatz publication-title: Orthopäde – volume: 7 start-page: 286 year: 2000 end-page: 291 ident: RF20 article-title: Efficient lipid-mediated gene transfer to articular chondrocytes publication-title: Gene Therapy – volume: 28 start-page: 891 year: 1994 end-page: 899 ident: RF4 article-title: Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffolds publication-title: J Biomed Mat Res – volume: 249 start-page: 248 year: 1999 end-page: 259 ident: RF21 article-title: SV40 large T antigen expression driven by publication-title: Experimental Cell Research – start-page: 173 year: 1999 end-page: 192 ident: RF24 article-title: Preparation of immortalized human chondrocyte cell lines publication-title: Tissue Engineering Methods and Protocols, Vol. 18 – start-page: 627 year: 1995 end-page: 647 ident: RF33 article-title: Biologic biomaterials: tissue-derived biomaterials (collagen) publication-title: The Biomedical Engineering Handbook – volume: 41 start-page: 1331 year: 1998 end-page: 1342 ident: RF1 article-title: Articular cartilage repair and transplantation publication-title: Arthritis Rheum – volume: 6 start-page: 50 year: 1998 end-page: 65 ident: RF25 article-title: Transplantation of allograft chondrocytes embedded in agarose gel into cartilage defects of rabbits publication-title: Osteoarthritis Cart – volume: 331 start-page: 889 year: 1994 end-page: 895 ident: RF3 article-title: Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation publication-title: New Engl J Med – volume: 176 start-page: 303 year: 1998 end-page: 313 ident: RF7 article-title: Dedifferentiated chondrocytes cultured in alginate beads: restoration of the differentiated phenotype and of the metabolic responses to Interleukin-1β publication-title: J Cell Physiol – volume: 27 start-page: 983 year: 2000 end-page: 989 ident: RF13 article-title: Empirical advantages of adeno associated viral vectors for publication-title: J Rheumatol – volume: 302 start-page: 233 year: 1999 end-page: 250 ident: RF17 article-title: Expression of green fluorescent protein in transgenic mice publication-title: Methods in Enzymology – volume: 6 start-page: 153 year: 1998 end-page: 159 ident: RF12 article-title: Resurfacing of articular cartilage explants with genetically-modified human chondrocytes publication-title: Osteoarthritis Cart – volume: 80 start-page: 3049 year: 1999 end-page: 3064 ident: RF27 article-title: Retrovirus receptors publication-title: J Gen Virol – volume: 67 start-page: 523 year: 1996 end-page: 529 ident: RF6 article-title: Cartilage repair. A critical review publication-title: Acta Orthopaedica Scandinavica – volume: 70 start-page: 5564 year: 1996 end-page: 5571 ident: RF28 article-title: Retrovirus packaging cells based on 10A1 murine leukemia virus for production of vectors that use multiple receptors for cell entry publication-title: J Virol – volume: 240 start-page: 58 year: 1998 end-page: 65 ident: RF9 article-title: Chondrogenesis in a cell-polymer-bioreactor system publication-title: Experimental Cell Res – volume: 25 start-page: 1666 year: 1998 end-page: 1673 ident: RF23 article-title: Adenovirus mediated gene delivery to the joints of guinea pigs publication-title: J Rheumatol – volume: 79 start-page: 1439 year: 1997 ident: 10.1053/joca.2001.0486_RF5 article-title: Effect of cultured autologous chondrocytes on repair of chondral defects in a canine model publication-title: J Bone and Joint Surg doi: 10.2106/00004623-199710000-00001 – volume: 91 start-page: 431 year: 1998 ident: 10.1053/joca.2001.0486_RF29 article-title: Retrovirally transduced CD34++ human cord blood cells generate T cells expressing high levels of the retroviral encoded green fluorescent protein marker in vitro publication-title: Blood doi: 10.1182/blood.V91.2.431 – volume: 28 start-page: 68 year: 1999 ident: 10.1053/joca.2001.0486_RF31 article-title: Wachstumsverhalten von Chondrozyten auf unterschiedlichen Trägersubstanzen publication-title: Orthopäde – volume: 6 start-page: 153 year: 1998 ident: 10.1053/joca.2001.0486_RF12 article-title: Resurfacing of articular cartilage explants with genetically-modified human chondrocytes in vitro publication-title: Osteoarthritis Cart doi: 10.1053/joca.1998.0107 – volume: 27 start-page: 309 year: 1998 ident: 10.1053/joca.2001.0486_RF2 article-title: Knorpelregeneration und Knorpelersatz publication-title: Orthopäde doi: 10.1007/s001320050237 – volume: 27 start-page: 983 year: 2000 ident: 10.1053/joca.2001.0486_RF13 article-title: Empirical advantages of adeno associated viral vectors for in vivo gene therapy for arthritis publication-title: J Rheumatol – volume: 302 start-page: 233 year: 1999 ident: 10.1053/joca.2001.0486_RF17 article-title: Expression of green fluorescent protein in transgenic mice publication-title: Methods in Enzymology doi: 10.1016/S0076-6879(99)02023-6 – volume: 12 start-page: 1035 year: 1999 ident: 10.1053/joca.2001.0486_RF15 article-title: Attenuation of green fluorescent protein half-line in mammalian cells publication-title: Protein Engineering doi: 10.1093/protein/12.12.1035 – start-page: 611 year: 1995 ident: 10.1053/joca.2001.0486_RF34 article-title: Biodegradable polymeric biomaterials: An overview – volume: 5 start-page: 684 year: 1998 ident: 10.1053/joca.2001.0486_RF38 article-title: Generation of a conditionally neor containing retroviral producer cell line: effects ofneor on retroviral titer and transgene expression publication-title: Gene Therapy doi: 10.1038/sj.gt.3300654 – volume: 41 start-page: 1331 year: 1998 ident: 10.1053/joca.2001.0486_RF1 article-title: Articular cartilage repair and transplantation publication-title: Arthritis Rheum doi: 10.1002/1529-0131(199808)41:8<1331::AID-ART2>3.0.CO;2-J – volume: 25 start-page: 1666 year: 1998 ident: 10.1053/joca.2001.0486_RF23 article-title: Adenovirus mediated gene delivery to the joints of guinea pigs publication-title: J Rheumatol – volume: 16 start-page: 207 year: 1998 ident: 10.1053/joca.2001.0486_RF8 article-title: Expression of a stable articular cartilage phenotype without evidence of hypertrophy by adult human articular chondrocytes in vitro publication-title: J Orthopaedic Res doi: 10.1002/jor.1100160208 – volume: 61 start-page: 843 year: 1999 ident: 10.1053/joca.2001.0486_RF19 article-title: Fluorescence expression by bovine embryos after pronuclear microinjection with the EGFP gene publication-title: J Veterinary Med Sci doi: 10.1292/jvms.61.843 – start-page: 173 year: 1999 ident: 10.1053/joca.2001.0486_RF24 article-title: Preparation of immortalized human chondrocyte cell lines – volume: 10 start-page: 1893 year: 1999 ident: 10.1053/joca.2001.0486_RF30 article-title: Complement-protected amphotropic retroviruses from murine packaging cells publication-title: Human Gene Therapy doi: 10.1089/10430349950017572 – volume: 80 start-page: 3049 year: 1999 ident: 10.1053/joca.2001.0486_RF27 article-title: Retrovirus receptors publication-title: J Gen Virol doi: 10.1099/0022-1317-80-12-3049 – volume: 96 start-page: 2454 year: 1995 ident: 10.1053/joca.2001.0486_RF22 article-title: Transplantation of transduced chondrocytes protects articular cartilage from interleukin 1-induced extracellular matrix degradation publication-title: J Clin Invest doi: 10.1172/JCI118303 – volume: 5 start-page: 275 year: 1997 ident: 10.1053/joca.2001.0486_RF11 article-title: Transplantation of adenovirally transduced allogeneic chondrocytes into articular cartilage defects in vivo publication-title: Osteoarthritis Cart doi: 10.1016/S1063-4584(97)80023-4 – volume: 181 start-page: 513 year: 1999 ident: 10.1053/joca.2001.0486_RF26 article-title: Development of a biocomposite to fill out articular cartilage lesions. Light, scanning and transmission electron microscopy of sheep chondrocytes cultured on a collagen I/III sponge publication-title: Anatomischer Anzeiger doi: 10.1016/S0940-9602(99)80055-7 – volume: 249 start-page: 248 year: 1999 ident: 10.1053/joca.2001.0486_RF21 article-title: SV40 large T antigen expression driven by col2a1 regulatory sequences immortalizes articular chondrocytes but does not allow stabilization of type II collagen expression publication-title: Experimental Cell Research doi: 10.1006/excr.1999.4478 – volume: 67 start-page: 523 year: 1996 ident: 10.1053/joca.2001.0486_RF6 article-title: Cartilage repair. A critical review publication-title: Acta Orthopaedica Scandinavica doi: 10.3109/17453679608996682 – volume: 6 start-page: 50 year: 1998 ident: 10.1053/joca.2001.0486_RF25 article-title: Transplantation of allograft chondrocytes embedded in agarose gel into cartilage defects of rabbits publication-title: Osteoarthritis Cart doi: 10.1053/joca.1997.0092 – volume: 28 start-page: 891 year: 1994 ident: 10.1053/joca.2001.0486_RF4 article-title: Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffolds publication-title: J Biomed Mat Res doi: 10.1002/jbm.820280808 – volume: 167 start-page: 95 year: 2000 ident: 10.1053/joca.2001.0486_RF35 article-title: Bioartificial cartilage publication-title: Cells Tissues Organs doi: 10.1159/000016773 – volume: 176 start-page: 303 year: 1998 ident: 10.1053/joca.2001.0486_RF7 article-title: Dedifferentiated chondrocytes cultured in alginate beads: restoration of the differentiated phenotype and of the metabolic responses to Interleukin-1β publication-title: J Cell Physiol doi: 10.1002/(SICI)1097-4652(199808)176:2<303::AID-JCP8>3.0.CO;2-S – volume: 70 start-page: 5564 year: 1996 ident: 10.1053/joca.2001.0486_RF28 article-title: Retrovirus packaging cells based on 10A1 murine leukemia virus for production of vectors that use multiple receptors for cell entry publication-title: J Virol doi: 10.1128/jvi.70.8.5564-5571.1996 – volume: 15 start-page: 458 year: 1997 ident: 10.1053/joca.2001.0486_RF16 article-title: Selective production of transgenic mice using green fluorescent protein as a marker publication-title: Nature Biotech doi: 10.1038/nbt0597-458 – volume: 71 start-page: 7533 year: 1997 ident: 10.1053/joca.2001.0486_RF37 article-title: Cre/loxP-mediated excision of a neomycin resistance expression unit from an integrated retroviral vector increases long terminal repeat-driven transcription in human hematopoietic cells publication-title: J Virol doi: 10.1128/jvi.71.10.7533-7540.1997 – volume: 5 start-page: 139, 43 year: 1997 ident: 10.1053/joca.2001.0486_RF10 article-title: Ex vivo gene transfer to chondrocytes in full-thickness articular cartilage defects: a feasibility study publication-title: Osteoarthritis Cart doi: 10.1016/S1063-4584(97)80007-6 – volume: 331 start-page: 889 year: 1994 ident: 10.1053/joca.2001.0486_RF3 article-title: Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation publication-title: New Engl J Med doi: 10.1056/NEJM199410063311401 – volume: 302 start-page: 358 year: 1999 ident: 10.1053/joca.2001.0486_RF18 article-title: In vivo retroviral transduction and expression of green fluorescent protein publication-title: Methods in Enzymology doi: 10.1016/S0076-6879(99)02032-7 – volume: 240 start-page: 58 year: 1998 ident: 10.1053/joca.2001.0486_RF9 article-title: Chondrogenesis in a cell-polymer-bioreactor system publication-title: Experimental Cell Res doi: 10.1006/excr.1998.4010 – volume: 7 start-page: 286 year: 2000 ident: 10.1053/joca.2001.0486_RF20 article-title: Efficient lipid-mediated gene transfer to articular chondrocytes publication-title: Gene Therapy doi: 10.1038/sj.gt.3301086 – volume: 42 start-page: 347 year: 1998 ident: 10.1053/joca.2001.0486_RF32 article-title: Cartilage reconstruction in head and neck surgery: comparison of resorbable polymer scaffolds for tissue engineering of human septal cartilage publication-title: J Biomed Mat Res doi: 10.1002/(SICI)1097-4636(19981205)42:3<347::AID-JBM2>3.0.CO;2-J – start-page: 627 year: 1995 ident: 10.1053/joca.2001.0486_RF33 article-title: Biologic biomaterials: tissue-derived biomaterials (collagen) – volume: 67 start-page: 509 year: 1998 ident: 10.1053/joca.2001.0486_RF14 article-title: The green fluorescent protein publication-title: Ann Rev Biochem doi: 10.1146/annurev.biochem.67.1.509 – volume: 99 start-page: 249 year: 1991 ident: 10.1053/joca.2001.0486_RF36 article-title: The prokaryotic neomycin-resistance-encoding gene acts as a transcriptional silencer in eukaryotic cells publication-title: Gene doi: 10.1016/0378-1119(91)90134-W |
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| Snippet | Objective One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for... One of the main open questions in chondrocyte transplantation is the fate of the implanted cells in vivo. We intended to establish prerequisites for such... |
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| SubjectTerms | Animals Articular chondrocytes, Retrovirus, GFP marking, Cell transplantation Cartilage, Articular - cytology Cattle Cell Separation Cells, Cultured Chondrocytes - physiology Chondrocytes - transplantation Feasibility Studies Flow Cytometry Gene Expression Genetic Markers Green Fluorescent Proteins Humans Leukemia Virus, Murine - genetics Luminescent Proteins - genetics Microscopy, Confocal Microscopy, Fluorescence Rabbits Reverse Transcriptase Polymerase Chain Reaction Sheep |
| Title | Vital marking of articular chondrocytes by retroviral infection using green fluorescence protein |
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