Characterization of microsatellite loci in Himatanthus drasticus (Apocynaceae), a medicinal plant from the Brazilian savanna

• Premise of the study: We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk medicine in northeastern Brazil. These novel markers are being used to evaluate the effect of harvesting on the genetic s...

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Veröffentlicht in:	American journal of botany Jg. 98; H. 9; S. e244 - e246
Hauptverfasser:	Baldauf, Cristina, Ciampi, Maísa B., Vigna, Bianca B. Z., Mori, Gustavo M., Guedes, Julian P. P., De Souza, Anete P., Santos, Flavio A. M. dos
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Botanical Society of America 01.09.2011 Botanical Society of America, Inc
Schlagworte:	alleles Apocynaceae Apocynaceae - genetics Base Sequence Botany Brazil conservation DNA Primers Genes, Plant Genetic structure genetic variation Genetics Genomes genomic libraries harvesting heterozygosity Heterozygote loci Medicinal plants microsatellite repeats Microsatellite Repeats - genetics molecular markers Natural populations Plants, Medicinal - genetics Population genetics Population studies savannas Sustainability management Sustainable development sustainable management traditional medicine Trees Brazil
ISSN:	0002-9122, 1537-2197, 1537-2197
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Abstract	• Premise of the study: We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk medicine in northeastern Brazil. These novel markers are being used to evaluate the effect of harvesting on the genetic structure and diversity of natural populations of this species. • Methods and Results: Microsatellite loci were isolated from an enriched H. drasticus genomic library. Nine primer pairs successfully amplified polymorphic microsatellite regions, with an average of 8.5 alleles per locus. The average values of observed and expected heterozygosity were 0.456 and 0.601, respectively. • Conclusions: The microsatellite markers described here are valuable tools for population genetics studies of H. drasticus. The majority of the primers also amplified sequences in the genome of another species of the same genus. This new set of markers may be useful in designing a genetic conservation strategy and a sustainable management plan for the species.
AbstractList	• Premise of the study: We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk medicine in northeastern Brazil. These novel markers are being used to evaluate the effect of harvesting on the genetic structure and diversity of natural populations of this species. • METHODS: and Results: Microsatellite loci were isolated from an enriched H. drasticus genomic library. Nine primer pairs successfully amplified polymorphic microsatellite regions, with an average of 8.5 alleles per locus. The average values of observed and expected heterozygosity were 0.456 and 0.601, respectively. • CONCLUSIONS: The microsatellite markers described here are valuable tools for population genetics studies of H. drasticus. The majority of the primers also amplified sequences in the genome of another species of the same genus. This new set of markers may be useful in designing a genetic conservation strategy and a sustainable management plan for the species. We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk medicine in northeastern Brazil. These novel markers are being used to evaluate the effect of harvesting on the genetic structure and diversity of natural populations of this species. • Microsatellite loci were isolated from an enriched H. drasticus genomic library. Nine primer pairs successfully amplified polymorphic microsatellite regions, with an average of 8.5 alleles per locus. The average values of observed and expected heterozygosity were 0.456 and 0.601, respectively. • The microsatellite markers described here are valuable tools for population genetics studies of H. drasticus. The majority of the primers also amplified sequences in the genome of another species of the same genus. This new set of markers may be useful in designing a genetic conservation strategy and a sustainable management plan for the species. We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk medicine in northeastern Brazil. These novel markers are being used to evaluate the effect of harvesting on the genetic structure and diversity of natural populations of this species. •PREMISE OF THE STUDYWe developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk medicine in northeastern Brazil. These novel markers are being used to evaluate the effect of harvesting on the genetic structure and diversity of natural populations of this species. •Microsatellite loci were isolated from an enriched H. drasticus genomic library. Nine primer pairs successfully amplified polymorphic microsatellite regions, with an average of 8.5 alleles per locus. The average values of observed and expected heterozygosity were 0.456 and 0.601, respectively. •METHODS AND RESULTSMicrosatellite loci were isolated from an enriched H. drasticus genomic library. Nine primer pairs successfully amplified polymorphic microsatellite regions, with an average of 8.5 alleles per locus. The average values of observed and expected heterozygosity were 0.456 and 0.601, respectively. •The microsatellite markers described here are valuable tools for population genetics studies of H. drasticus. The majority of the primers also amplified sequences in the genome of another species of the same genus. This new set of markers may be useful in designing a genetic conservation strategy and a sustainable management plan for the species.CONCLUSIONSThe microsatellite markers described here are valuable tools for population genetics studies of H. drasticus. The majority of the primers also amplified sequences in the genome of another species of the same genus. This new set of markers may be useful in designing a genetic conservation strategy and a sustainable management plan for the species. We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk medicine in northeastern Brazil. These novel markers are being used to evaluate the effect of harvesting on the genetic structure and diversity of natural populations of this species. Microsatellite loci were isolated from an enriched H. drasticus genomic library. Nine primer pairs successfully amplified polymorphic microsatellite regions, with an average of 8.5 alleles per locus. The average values of observed and expected heterozygosity were 0.456 and 0.601, respectively. The microsatellite markers described here are valuable tools for population genetics studies of H. drasticus. The majority of the primers also amplified sequences in the genome of another species of the same genus. This new set of markers may be useful in designing a genetic conservation strategy and a sustainable management plan for the species. • Premise of the study: We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk medicine in northeastern Brazil. These novel markers are being used to evaluate the effect of harvesting on the genetic structure and diversity of natural populations of this species. • Methods and Results: Microsatellite loci were isolated from an enriched H. drasticus genomic library. Nine primer pairs successfully amplified polymorphic microsatellite regions, with an average of 8.5 alleles per locus. The average values of observed and expected heterozygosity were 0.456 and 0.601, respectively. • Conclusions: The microsatellite markers described here are valuable tools for population genetics studies of H. drasticus . The majority of the primers also amplified sequences in the genome of another species of the same genus. This new set of markers may be useful in designing a genetic conservation strategy and a sustainable management plan for the species.
Author	Ciampi, Maísa B. Baldauf, Cristina Vigna, Bianca B. Z. De Souza, Anete P. Mori, Gustavo M. Guedes, Julian P. P. Santos, Flavio A. M. dos
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Cites_doi	10.1111/j.1365-2664.2004.00859.x 10.1111/j.1365-294X.2006.03067.x 10.1093/jhered/93.5.365 10.1101/gr.184001 10.3923/ijb.2009.171.175 10.1093/nar/gkm306
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Notes	The authors would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, No. 472127/2008‐0, 304937/2007‐0, and 140813/2008‐0) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, No. 2008/08747‐4) for financial support. We are also grateful to T. Eugênio, A. Staffocker, and P. Zambon for technical support. SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23
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Snippet	• Premise of the study: We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel,... We developed a new set of microsatellite markers for studying the genome of the janaguba tree, Himatanthus drasticus (Mart.) Plumel, which is used in folk...
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SubjectTerms	alleles Apocynaceae Apocynaceae - genetics Base Sequence Botany Brazil conservation DNA Primers Genes, Plant Genetic structure genetic variation Genetics Genomes genomic libraries harvesting heterozygosity Heterozygote loci Medicinal plants microsatellite repeats Microsatellite Repeats - genetics molecular markers Natural populations Plants, Medicinal - genetics Population genetics Population studies savannas Sustainability management Sustainable development sustainable management traditional medicine Trees
Title	Characterization of microsatellite loci in Himatanthus drasticus (Apocynaceae), a medicinal plant from the Brazilian savanna
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