Label-free aptasensor for the detection of cardiac biomarker myoglobin based on gold nanoparticles decorated boron nitride nanosheets

A novel electrochemical aptasensor based on gold nanoparticles decorated on boron nitride nanosheets (AuNPs/BNNSs) for the sensitive and selective detection of myoglobin (Mb) is reported. BNNSs were chemically synthesized by a low-cost and simple hydrothermal method. They were deposited onto the flu...

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Published in:Biosensors & bioelectronics Vol. 126; pp. 143 - 150
Main Authors: Adeel, Muhammad, Rahman, Md. Mahbubur, Lee, Jae-Joon
Format: Journal Article
Language:English
Published: England Elsevier B.V 01.02.2019
Subjects:
Aptasensor
aptasensors
Au nanoparticles
biomarkers
boron nitride
Boron nitride nanosheets
chemical bonding
chemical reduction
DNA
electrochemistry
electrodes
High sensitivity
hot water treatment
iron
Myoglobin
nanogold
nanosheets
oligonucleotides
oxidation
particle size
point-of-care testing
tin dioxide
Myoglobin
Boron nitride nanosheets
Au nanoparticles
High sensitivity
Aptasensor
ISSN:0956-5663, 1873-4235, 1873-4235
Online Access:Get full text
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Summary:A novel electrochemical aptasensor based on gold nanoparticles decorated on boron nitride nanosheets (AuNPs/BNNSs) for the sensitive and selective detection of myoglobin (Mb) is reported. BNNSs were chemically synthesized by a low-cost and simple hydrothermal method. They were deposited onto the fluorine-doped tin oxide (FTO) electrode by a spin-coating method. Subsequently, AuNPs were chemically deposited onto the BNNS/FTO electrode by a seed-mediated chemical reduction method, with an average particle size of approximately 10 nm. The AuNPs/BNNSs/FTO electrode was used as a transducer to immobilize a thiol-functionalized DNA aptamer (Apt) via the covalent interaction of Au–S for the specific binding of Mb. [Fe(CN)6]3-/4- was used as a redox probe to monitor the oxidation current variation upon the binding of Mb with varying concentrations onto the sensor surface. The Apt/AuNPs/BNNSs/FTO sensor shows a high signal response for Mb with a detection limit of 34.6 ng/mL and a dynamic response range of 0.1–100 µg/mL. It is a promising candidate for point-of-care diagnosis in real samples. This strategy could make possible the application of other 2D materials with wide bandgaps for the development of biosensors. •AuNP/BNNS-modified FTO electrode is prepared for the aptamer-based detection of Mb.•AuNP/BNNS/FTO is used as a transducer to immobilize thiol-functionalized aptamer.•The immobilized aptamers allow the specific binding of target Mb biomarkers.•Variation of the oxidation signals of [Fe(CN)6]3-/4- enables the detection of Mb.•The aptasensor showed high performance for the sensitive detection of Mb.
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ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/j.bios.2018.10.060