Complete mitochondrial genome sequence of the Tyrolean Iceman

The Tyrolean Iceman was a witness to the Neolithic-Copper Age transition in Central Europe 5350-5100 years ago, and his mummified corpse was recovered from an Alpine glacier on the Austro-Italian border in 1991 [1]. Using a mixed sequencing procedure based on PCR amplification and 454 sequencing of...

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Vydáno v:Current biology Ročník 18; číslo 21; s. 1687
Hlavní autoři: Ermini, Luca, Olivieri, Cristina, Rizzi, Ermanno, Corti, Giorgio, Bonnal, Raoul, Soares, Pedro, Luciani, Stefania, Marota, Isolina, De Bellis, Gianluca, Richards, Martin B, Rollo, Franco
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 11.11.2008
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ISSN:0960-9822
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Abstract The Tyrolean Iceman was a witness to the Neolithic-Copper Age transition in Central Europe 5350-5100 years ago, and his mummified corpse was recovered from an Alpine glacier on the Austro-Italian border in 1991 [1]. Using a mixed sequencing procedure based on PCR amplification and 454 sequencing of pooled amplification products, we have retrieved the first complete mitochondrial-genome sequence of a prehistoric European. We have then compared it with 115 related extant lineages from mitochondrial haplogroup K. We found that the Iceman belonged to a branch of mitochondrial haplogroup K1 that has not yet been identified in modern European populations. This is the oldest complete Homo sapiens mtDNA genome generated to date. The results point to the potential significance of complete-ancient-mtDNA studies in addressing questions concerning the genetic history of human populations that the phylogeography of modern lineages is unable to tackle.
AbstractList The Tyrolean Iceman was a witness to the Neolithic-Copper Age transition in Central Europe 5350-5100 years ago, and his mummified corpse was recovered from an Alpine glacier on the Austro-Italian border in 1991 [1]. Using a mixed sequencing procedure based on PCR amplification and 454 sequencing of pooled amplification products, we have retrieved the first complete mitochondrial-genome sequence of a prehistoric European. We have then compared it with 115 related extant lineages from mitochondrial haplogroup K. We found that the Iceman belonged to a branch of mitochondrial haplogroup K1 that has not yet been identified in modern European populations. This is the oldest complete Homo sapiens mtDNA genome generated to date. The results point to the potential significance of complete-ancient-mtDNA studies in addressing questions concerning the genetic history of human populations that the phylogeography of modern lineages is unable to tackle.The Tyrolean Iceman was a witness to the Neolithic-Copper Age transition in Central Europe 5350-5100 years ago, and his mummified corpse was recovered from an Alpine glacier on the Austro-Italian border in 1991 [1]. Using a mixed sequencing procedure based on PCR amplification and 454 sequencing of pooled amplification products, we have retrieved the first complete mitochondrial-genome sequence of a prehistoric European. We have then compared it with 115 related extant lineages from mitochondrial haplogroup K. We found that the Iceman belonged to a branch of mitochondrial haplogroup K1 that has not yet been identified in modern European populations. This is the oldest complete Homo sapiens mtDNA genome generated to date. The results point to the potential significance of complete-ancient-mtDNA studies in addressing questions concerning the genetic history of human populations that the phylogeography of modern lineages is unable to tackle.
The Tyrolean Iceman was a witness to the Neolithic-Copper Age transition in Central Europe 5350-5100 years ago, and his mummified corpse was recovered from an Alpine glacier on the Austro-Italian border in 1991 [1]. Using a mixed sequencing procedure based on PCR amplification and 454 sequencing of pooled amplification products, we have retrieved the first complete mitochondrial-genome sequence of a prehistoric European. We have then compared it with 115 related extant lineages from mitochondrial haplogroup K. We found that the Iceman belonged to a branch of mitochondrial haplogroup K1 that has not yet been identified in modern European populations. This is the oldest complete Homo sapiens mtDNA genome generated to date. The results point to the potential significance of complete-ancient-mtDNA studies in addressing questions concerning the genetic history of human populations that the phylogeography of modern lineages is unable to tackle.
Author Corti, Giorgio
Luciani, Stefania
Rizzi, Ermanno
Olivieri, Cristina
Ermini, Luca
Soares, Pedro
Bonnal, Raoul
De Bellis, Gianluca
Marota, Isolina
Richards, Martin B
Rollo, Franco
Author_xml – sequence: 1
  givenname: Luca
  surname: Ermini
  fullname: Ermini, Luca
  organization: Laboratorio di Archeo-Antropologia Molecolare/DNA Antico, Dipartimento di Biologia Molecolare, Cellulare e Animale, University of Camerino, 62032 Camerino, Italy
– sequence: 2
  givenname: Cristina
  surname: Olivieri
  fullname: Olivieri, Cristina
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  givenname: Ermanno
  surname: Rizzi
  fullname: Rizzi, Ermanno
– sequence: 4
  givenname: Giorgio
  surname: Corti
  fullname: Corti, Giorgio
– sequence: 5
  givenname: Raoul
  surname: Bonnal
  fullname: Bonnal, Raoul
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  givenname: Pedro
  surname: Soares
  fullname: Soares, Pedro
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  givenname: Stefania
  surname: Luciani
  fullname: Luciani, Stefania
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  givenname: Isolina
  surname: Marota
  fullname: Marota, Isolina
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  givenname: Gianluca
  surname: De Bellis
  fullname: De Bellis, Gianluca
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  givenname: Martin B
  surname: Richards
  fullname: Richards, Martin B
– sequence: 11
  givenname: Franco
  surname: Rollo
  fullname: Rollo, Franco
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Snippet The Tyrolean Iceman was a witness to the Neolithic-Copper Age transition in Central Europe 5350-5100 years ago, and his mummified corpse was recovered from an...
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SubjectTerms DNA, Mitochondrial - genetics
Genome, Mitochondrial
Humans
Male
Mummies
Phylogeny
Preservation, Biological
Sequence Analysis, DNA
Title Complete mitochondrial genome sequence of the Tyrolean Iceman
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