Bench-scale study of the effect of phosphate on an aerobic iron oxidation plant for mine water treatment

At the opencast pit Nochten acidic iron- and sulfate-rich mine waters are treated biotechnologically in a mine-water treatment plant by microbial iron oxidation. Due to the low phosphate concentration in such waters the treatment plant was simulated in bench-scale to investigate the influence of add...

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Bibliographic Details
Published in:Water research (Oxford) Vol. 48; pp. 345 - 353
Main Authors: Tischler, Judith S., Wiacek, Claudia, Janneck, Eberhard, Schlömann, Michael
Format: Journal Article
Language:English
Published: Kidlington Elsevier Ltd 01.01.2014
Elsevier
Subjects:
Acid mine water
Acidiphilium
Acidithiobacillus
Aerobiosis
Applied sciences
bacteria
Biological and medical sciences
Biological treatment of waters
Bioremediation
Biotechnology
DNA - genetics
Environment and pollution
Exact sciences and technology
Ferrovum
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Industrial wastewaters
Iron - chemistry
microbial communities
Microbial iron oxidation
Mining
oxidation
Oxidation-Reduction
Phosphate limitation
Phosphates - chemistry
Pollution
Polymorphism, Restriction Fragment Length
potassium dihydrogen phosphate
restriction fragment length polymorphism
Wastewaters
Water Purification - methods
water treatment
Water treatment and pollution
Europe
Germany
Phosphate limitation
Bioremediation
Acid mine water
Ferrovum
Microbial iron oxidation
Water treatment
Microbiology
Dihydrogenphosphates
Iron
Aerobe
Biological purification
Iron-oxidizing bacteria
Industrial waste water
Limiting factor
Oxidation
Waste water purification
Mining industry
Microbial community
ISSN:0043-1354, 1879-2448, 1879-2448
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Summary:At the opencast pit Nochten acidic iron- and sulfate-rich mine waters are treated biotechnologically in a mine-water treatment plant by microbial iron oxidation. Due to the low phosphate concentration in such waters the treatment plant was simulated in bench-scale to investigate the influence of addition of potassium dihydrogen phosphate on chemical and biological parameters of the mine-water treatment. As a result of the phosphate addition the number of cells increased, which resulted in an increase of the iron oxidation rate in the reactor with phosphate addition by a factor of 1.7 compared to a reference approach without phosphate addition. Terminal restriction fragment length polymorphism (T-RFLP) analysis during the cultivation revealed a shift of the microbial community depending on the phosphate addition. While almost exclusively iron-oxidizing bacteria related to “Ferrovum” sp. were detected with phosphate addition, the microbial community was more diverse without phosphate addition. In the latter case, iron-oxidizing bacteria (“Ferrovum” sp., Acidithiobacillus spp.) as well as non-iron-oxidizing bacteria (Acidiphilium sp.) were identified. [Display omitted] •Simulation of biological remediation of acid mine waters in bench-scale.•Optimization of bioremediation of acid mine waters by phosphate addition.•Limitation of “Ferrovum” spp. by phosphate in acid mine waters.
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ISSN:0043-1354
1879-2448
1879-2448
DOI:10.1016/j.watres.2013.09.049