An evolution-based model for designing chorismate mutase enzymes

The rational design of enzymes is an important goal for both fundamental and practical reasons. Here, we describe a process to learn the constraints for specifying proteins purely from evolutionary sequence data, design and build libraries of synthetic genes, and test them for activity in vivo using...

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Bibliographic Details

Published in:	Science (American Association for the Advancement of Science) Vol. 369; no. 6502; p. 440
Main Authors:	Russ, William P, Figliuzzi, Matteo, Stocker, Christian, Barrat-Charlaix, Pierre, Socolich, Michael, Kast, Peter, Hilvert, Donald, Monasson, Remi, Cocco, Simona, Weigt, Martin, Ranganathan, Rama
Format:	Journal Article
Language:	English
Published:	United States 24.07.2020
Subjects:	Amino Acid Sequence Chorismate Mutase - chemistry Chorismate Mutase - genetics Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Evolution, Molecular Models, Genetic Models, Statistical
ISSN:	1095-9203, 1095-9203
Online Access:	Get more information
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