Intravital imaging technology guides FAK-mediated priming in pancreatic cancer precision medicine according to Merlin status
Intravital imaging guides a personalized medicine approach to target mechanoreciprocity in pancreatic cancer. Pancreatic ductal adenocarcinoma (PDAC) is a highly metastatic, chemoresistant malignancy and is characterized by a dense, desmoplastic stroma that modulates PDAC progression. Here, we visua...
Saved in:
| Published in: | Science advances Vol. 7; no. 40; p. eabh0363 |
|---|---|
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
American Association for the Advancement of Science (AAAS)
01.10.2021
American Association for the Advancement of Science |
| Subjects: | |
| ISSN: | 2375-2548, 2375-2548 |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Intravital imaging guides a personalized medicine approach to target mechanoreciprocity in pancreatic cancer.
Pancreatic ductal adenocarcinoma (PDAC) is a highly metastatic, chemoresistant malignancy and is characterized by a dense, desmoplastic stroma that modulates PDAC progression. Here, we visualized transient manipulation of focal adhesion kinase (FAK), which integrates bidirectional cell-environment signaling, using intravital fluorescence lifetime imaging microscopy of the FAK-based Förster resonance energy transfer biosensor in mouse and patient-derived PDAC models. Parallel real-time quantification of the FUCCI cell cycle reporter guided us to improve PDAC response to standard-of-care chemotherapy at primary and secondary sites. Critically, micropatterned pillar plates and stiffness-tunable matrices were used to pinpoint the contribution of environmental cues to chemosensitization, while fluid flow–induced shear stress assessment, patient-derived matrices, and personalized in vivo models allowed us to deconstruct how FAK inhibition can reduce PDAC spread. Last, stratification of PDAC patient samples via Merlin status revealed a patient subset with poor prognosis that are likely to respond to FAK priming before chemotherapy. |
|---|---|
| AbstractList | Intravital imaging guides a personalized medicine approach to target mechanoreciprocity in pancreatic cancer. Pancreatic ductal adenocarcinoma (PDAC) is a highly metastatic, chemoresistant malignancy and is characterized by a dense, desmoplastic stroma that modulates PDAC progression. Here, we visualized transient manipulation of focal adhesion kinase (FAK), which integrates bidirectional cell-environment signaling, using intravital fluorescence lifetime imaging microscopy of the FAK-based Förster resonance energy transfer biosensor in mouse and patient-derived PDAC models. Parallel real-time quantification of the FUCCI cell cycle reporter guided us to improve PDAC response to standard-of-care chemotherapy at primary and secondary sites. Critically, micropatterned pillar plates and stiffness-tunable matrices were used to pinpoint the contribution of environmental cues to chemosensitization, while fluid flow–induced shear stress assessment, patient-derived matrices, and personalized in vivo models allowed us to deconstruct how FAK inhibition can reduce PDAC spread. Last, stratification of PDAC patient samples via Merlin status revealed a patient subset with poor prognosis that are likely to respond to FAK priming before chemotherapy. Pancreatic ductal adenocarcinoma (PDAC) is a highly metastatic, chemoresistant malignancy and is characterized by a dense, desmoplastic stroma that modulates PDAC progression. Here, we visualized transient manipulation of focal adhesion kinase (FAK), which integrates bidirectional cell-environment signaling, using intravital fluorescence lifetime imaging microscopy of the FAK-based Förster resonance energy transfer biosensor in mouse and patient-derived PDAC models. Parallel real-time quantification of the FUCCI cell cycle reporter guided us to improve PDAC response to standard-of-care chemotherapy at primary and secondary sites. Critically, micropatterned pillar plates and stiffness-tunable matrices were used to pinpoint the contribution of environmental cues to chemosensitization, while fluid flow–induced shear stress assessment, patient-derived matrices, and personalized in vivo models allowed us to deconstruct how FAK inhibition can reduce PDAC spread. Last, stratification of PDAC patient samples via Merlin status revealed a patient subset with poor prognosis that are likely to respond to FAK priming before chemotherapy. Pancreatic ductal adenocarcinoma (PDAC) is a highly metastatic, chemoresistant malignancy and is characterized by a dense, desmoplastic stroma that modulates PDAC progression. Here, we visualized transient manipulation of focal adhesion kinase (FAK), which integrates bidirectional cell-environment signaling, using intravital fluorescence lifetime imaging microscopy of the FAK-based Förster resonance energy transfer biosensor in mouse and patient-derived PDAC models. Parallel real-time quantification of the FUCCI cell cycle reporter guided us to improve PDAC response to standard-of-care chemotherapy at primary and secondary sites. Critically, micropatterned pillar plates and stiffness-tunable matrices were used to pinpoint the contribution of environmental cues to chemosensitization, while fluid flow–induced shear stress assessment, patient-derived matrices, and personalized in vivo models allowed us to deconstruct how FAK inhibition can reduce PDAC spread. Last, stratification of PDAC patient samples via Merlin status revealed a patient subset with poor prognosis that are likely to respond to FAK priming before chemotherapy.Pancreatic ductal adenocarcinoma (PDAC) is a highly metastatic, chemoresistant malignancy and is characterized by a dense, desmoplastic stroma that modulates PDAC progression. Here, we visualized transient manipulation of focal adhesion kinase (FAK), which integrates bidirectional cell-environment signaling, using intravital fluorescence lifetime imaging microscopy of the FAK-based Förster resonance energy transfer biosensor in mouse and patient-derived PDAC models. Parallel real-time quantification of the FUCCI cell cycle reporter guided us to improve PDAC response to standard-of-care chemotherapy at primary and secondary sites. Critically, micropatterned pillar plates and stiffness-tunable matrices were used to pinpoint the contribution of environmental cues to chemosensitization, while fluid flow–induced shear stress assessment, patient-derived matrices, and personalized in vivo models allowed us to deconstruct how FAK inhibition can reduce PDAC spread. Last, stratification of PDAC patient samples via Merlin status revealed a patient subset with poor prognosis that are likely to respond to FAK priming before chemotherapy. Intravital imaging guides a personalized medicine approach to target mechanoreciprocity in pancreatic cancer. Pancreatic ductal adenocarcinoma (PDAC) is a highly metastatic, chemoresistant malignancy and is characterized by a dense, desmoplastic stroma that modulates PDAC progression. Here, we visualized transient manipulation of focal adhesion kinase (FAK), which integrates bidirectional cell-environment signaling, using intravital fluorescence lifetime imaging microscopy of the FAK-based Förster resonance energy transfer biosensor in mouse and patient-derived PDAC models. Parallel real-time quantification of the FUCCI cell cycle reporter guided us to improve PDAC response to standard-of-care chemotherapy at primary and secondary sites. Critically, micropatterned pillar plates and stiffness-tunable matrices were used to pinpoint the contribution of environmental cues to chemosensitization, while fluid flow–induced shear stress assessment, patient-derived matrices, and personalized in vivo models allowed us to deconstruct how FAK inhibition can reduce PDAC spread. Last, stratification of PDAC patient samples via Merlin status revealed a patient subset with poor prognosis that are likely to respond to FAK priming before chemotherapy. |
| Author | Conway, James R. W. Nobis, Max Tayao, Michael Zaratzian, Anaiis Burgess, Andrew Da Silva, Andrew Wang, Yingxiao Chambers, Cecilia R. Pereira, Brooke A. Stoehr, Janett Magenau, Astrid Warren, Sean C. Gadegaard, Nikolaj Vennin, Claire Lucas, Morghan C. Abdulkhalek, Lea Pajic, Marina Cox, Thomas R. Chou, Angela Filipe, Elysse C. Horvath, Lisa G. Samra, Jaswinder Goetz, Jacky G. Samuel, Michael S. Reed, Daniel A. Timpson, Paul Skhinas, Joanna N. Murphy, Kendelle J. Lee, Victoria Metcalf, Xanthe L. Lyons, Ruth J. Yin, Julia X. Lyons, J. Guy Bidanel, Romain Morton, Jennifer P. Daly, Roger J. Biankin, Andrew V. Herrmann, David Grimmond, Sean M. Sansom, Owen J. Trpceski, Michael Gill, Anthony J. Johns, Amber L. Caldon, C. Elizabeth Major, Gretel Ritchie, Shona Parkin, Ashleigh |
| Author_xml | – sequence: 1 givenname: Kendelle J. orcidid: 0000-0002-3234-3812 surname: Murphy fullname: Murphy, Kendelle J. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 2 givenname: Daniel A. orcidid: 0000-0002-3736-7725 surname: Reed fullname: Reed, Daniel A. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 3 givenname: Claire surname: Vennin fullname: Vennin, Claire organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia., Oncode Institute, Division of Molecular Pathology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, Netherlands – sequence: 4 givenname: James R. W. orcidid: 0000-0003-3787-277X surname: Conway fullname: Conway, James R. W. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia., Turku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, Finland – sequence: 5 givenname: Max orcidid: 0000-0002-1861-1390 surname: Nobis fullname: Nobis, Max organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 6 givenname: Julia X. orcidid: 0000-0002-7706-5895 surname: Yin fullname: Yin, Julia X. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 7 givenname: Cecilia R. orcidid: 0000-0002-4979-9349 surname: Chambers fullname: Chambers, Cecilia R. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 8 givenname: Brooke A. orcidid: 0000-0003-3513-1214 surname: Pereira fullname: Pereira, Brooke A. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 9 givenname: Victoria orcidid: 0000-0002-0701-8321 surname: Lee fullname: Lee, Victoria organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 10 givenname: Elysse C. orcidid: 0000-0003-1956-0852 surname: Filipe fullname: Filipe, Elysse C. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 11 givenname: Michael surname: Trpceski fullname: Trpceski, Michael organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 12 givenname: Shona orcidid: 0000-0001-9234-0632 surname: Ritchie fullname: Ritchie, Shona organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 13 givenname: Morghan C. orcidid: 0000-0001-7654-9137 surname: Lucas fullname: Lucas, Morghan C. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 14 givenname: Sean C. orcidid: 0000-0002-5253-7147 surname: Warren fullname: Warren, Sean C. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 15 givenname: Joanna N. surname: Skhinas fullname: Skhinas, Joanna N. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 16 givenname: Astrid surname: Magenau fullname: Magenau, Astrid organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 17 givenname: Xanthe L. orcidid: 0000-0001-9599-9280 surname: Metcalf fullname: Metcalf, Xanthe L. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 18 givenname: Janett orcidid: 0000-0002-4198-3520 surname: Stoehr fullname: Stoehr, Janett organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 19 givenname: Gretel orcidid: 0000-0002-4275-4199 surname: Major fullname: Major, Gretel organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 20 givenname: Ashleigh surname: Parkin fullname: Parkin, Ashleigh organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 21 givenname: Romain surname: Bidanel fullname: Bidanel, Romain organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 22 givenname: Ruth J. surname: Lyons fullname: Lyons, Ruth J. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 23 givenname: Anaiis surname: Zaratzian fullname: Zaratzian, Anaiis organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 24 givenname: Michael orcidid: 0000-0001-5224-0784 surname: Tayao fullname: Tayao, Michael organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 25 givenname: Andrew surname: Da Silva fullname: Da Silva, Andrew organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 26 givenname: Lea surname: Abdulkhalek fullname: Abdulkhalek, Lea organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 27 givenname: Anthony J. orcidid: 0000-0002-9447-1967 surname: Gill fullname: Gill, Anthony J. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia., Sydney Medical School, University of Sydney, Sydney, NSW 2006, Australia., NSW Health Pathology, Department of Anatomical Pathology, Royal North Shore Hospital, NSW 2065, Australia., Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, NSW 2064, Australia – sequence: 28 givenname: Amber L. surname: Johns fullname: Johns, Amber L. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia – sequence: 29 givenname: Andrew V. orcidid: 0000-0002-0362-5597 surname: Biankin fullname: Biankin, Andrew V. organization: Institute of Cancer Sciences, Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Glasgow, UK., West of Scotland Pancreatic Unit, Glasgow Royal Infirmary, Glasgow, UK – sequence: 30 givenname: Jaswinder surname: Samra fullname: Samra, Jaswinder organization: Department of Surgery, Royal North Shore Hospital, Sydney, NSW 2065, Australia – sequence: 31 givenname: Sean M. orcidid: 0000-0002-8102-7998 surname: Grimmond fullname: Grimmond, Sean M. organization: University of Melbourne Centre for Cancer Research, Victorian Comprehensive Cancer Centre, 305 Grattan Street, Melbourne, VIC 3000, Australia – sequence: 32 givenname: Angela orcidid: 0000-0001-9212-6927 surname: Chou fullname: Chou, Angela organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., Sydney Medical School, University of Sydney, Sydney, NSW 2006, Australia., NSW Health Pathology, Department of Anatomical Pathology, Royal North Shore Hospital, NSW 2065, Australia., Cancer Diagnosis and Pathology Research Group, Kolling Institute of Medical Research, NSW 2064, Australia., Department of Anatomical Pathology, SydPath, Darlinghurst, NSW 2010, Australia – sequence: 33 givenname: Jacky G. orcidid: 0000-0003-2842-8116 surname: Goetz fullname: Goetz, Jacky G. organization: INSERM UMR, Tumour Biomechanics, Strasbourg, France., Université de Strasbourg, Strasbourg, France., Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg, France – sequence: 34 givenname: Michael S. orcidid: 0000-0001-7880-6379 surname: Samuel fullname: Samuel, Michael S. organization: Centre for Cancer Biology, SA Pathology and University of South Australia, SA, Australia., Adelaide Medical School, Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, SA, Australia – sequence: 35 givenname: J. Guy orcidid: 0000-0002-4835-4663 surname: Lyons fullname: Lyons, J. Guy organization: Dermatology, Sydney Medical School, University of Sydney, Camperdown, NSW, Australia., Cancer Services, Royal Prince Alfred Hospital, Camperdown, NSW, Australia., Centenary Institute, The University of Sydney, Camperdown, NSW, Australia – sequence: 36 givenname: Andrew orcidid: 0000-0003-4536-9226 surname: Burgess fullname: Burgess, Andrew organization: ANZAC Research Institute, Sydney, NSW 2139, Australia., Faculty of Medicine and Health, Concord Clinical School, University of Sydney, Sydney, NSW 2000, Australia – sequence: 37 givenname: C. Elizabeth orcidid: 0000-0003-1392-3472 surname: Caldon fullname: Caldon, C. Elizabeth organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 38 givenname: Lisa G. surname: Horvath fullname: Horvath, Lisa G. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia., Sydney Medical School, University of Sydney, Sydney, NSW 2006, Australia., Medical Oncology, Chris O’Brien Lifehouse, Camperdown, NSW 2006, Australia – sequence: 39 givenname: Roger J. orcidid: 0000-0002-5739-8027 surname: Daly fullname: Daly, Roger J. organization: Cancer Program and Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, VIC 3800, Australia – sequence: 40 givenname: Nikolaj orcidid: 0000-0002-3396-846X surname: Gadegaard fullname: Gadegaard, Nikolaj organization: James Watt School of Engineering, University of Glasgow, Glasgow, UK – sequence: 41 givenname: Yingxiao orcidid: 0000-0003-0265-326X surname: Wang fullname: Wang, Yingxiao organization: Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA – sequence: 42 givenname: Owen J. orcidid: 0000-0001-9540-3010 surname: Sansom fullname: Sansom, Owen J. organization: Institute of Cancer Sciences, Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Glasgow, UK., Cancer Research UK Beatson Institute, Glasgow, UK – sequence: 43 givenname: Jennifer P. orcidid: 0000-0001-5766-9141 surname: Morton fullname: Morton, Jennifer P. organization: Institute of Cancer Sciences, Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Glasgow, UK., Cancer Research UK Beatson Institute, Glasgow, UK – sequence: 44 givenname: Thomas R. orcidid: 0000-0001-9294-1745 surname: Cox fullname: Cox, Thomas R. organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 45 givenname: Marina orcidid: 0000-0002-3871-3829 surname: Pajic fullname: Pajic, Marina organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 46 givenname: David orcidid: 0000-0002-9514-7501 surname: Herrmann fullname: Herrmann, David organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia – sequence: 47 givenname: Paul orcidid: 0000-0002-5514-7080 surname: Timpson fullname: Timpson, Paul organization: Garvan Institute of Medical Research and The Kinghorn Cancer Centre, Cancer Division, Sydney, NSW 2010, Australia., St. Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34586840$$D View this record in MEDLINE/PubMed https://hal.science/hal-03957015$$DView record in HAL |
| BookMark | eNp1Uk1vEzEQtVARLaVXjshHOGyw1x_xXpCiitKKIC5wtpyxd2O0sYPtjVSJH4-XpKhU4uSR5703bz5eorMQg0PoNSULSlv5PoM39rAwmy1hkj1DFy1biqYVXJ09is_RVc4_CCGUSylo9wKdMy6UVJxcoF93oSRz8MWM2O_M4MOAi4NtiGMc7vEweesyvll9bnbOelOcxfvkdzPMB7w3AZIzxQOGGrpUkw589jHgGQ8-OGwAYrJ_hCP-4tJYibmYMuVX6HlvxuyuTu8l-n7z8dv1bbP--unuerVugLe8NH2vDCfESiDEAbPKWiksLC1h3IGjUtJO9hY46wRjShhGYSOMMIaxDeksu0Qfjrr7aVNtgZt7HvXciEn3Ohqv_80Ev9VDPGjFFekYqwLvjgLbJ7Tb1VrPf6SWXhIqDrRi356Kpfhzcrnonc_gxtEEF6esW7FUlHdKqAp989jXX-WH9VQAPwIgxZyT6zXUTZU63mrTj5oSPR-CPh6CPh1CpS2e0B6U_0P4DRBTuv4 |
| CitedBy_id | crossref_primary_10_1038_s41565_023_01535_8 crossref_primary_10_1186_s12951_024_02646_7 crossref_primary_10_1016_j_jid_2025_07_023 crossref_primary_10_1126_sciadv_adq4416 crossref_primary_10_1038_s41467_022_32255_7 crossref_primary_10_1038_s41556_025_01663_4 crossref_primary_10_3390_cancers15051480 crossref_primary_10_1002_ggn2_202200014 crossref_primary_10_1016_j_ajpath_2024_02_023 crossref_primary_10_1007_s44164_023_00043_2 crossref_primary_10_1242_jcs_261723 crossref_primary_10_1016_j_canlet_2024_217280 crossref_primary_10_1242_jcs_261549 crossref_primary_10_3390_cancers15030724 crossref_primary_10_1016_j_bbcan_2022_188756 crossref_primary_10_1136_gutjnl_2023_330049 crossref_primary_10_3390_cancers14122887 crossref_primary_10_1016_j_adcanc_2023_100096 crossref_primary_10_3389_fimmu_2023_1176594 crossref_primary_10_1042_BST20220162 crossref_primary_10_1016_j_jconrel_2025_114143 crossref_primary_10_1002_advs_202307963 crossref_primary_10_1073_pnas_2304288120 crossref_primary_10_3390_cancers15092448 crossref_primary_10_1007_s11684_023_1009_7 crossref_primary_10_1172_JCI157644 crossref_primary_10_1007_s13105_022_00941_1 crossref_primary_10_1038_s43586_022_00168_w |
| Cites_doi | 10.1038/nature14169 10.1038/ncb2775 10.1136/gutjnl-2018-316451 10.1126/science.1064829 10.1126/science.1171362 10.15252/emmm.202012010 10.1038/ncb2548 10.1126/scitranslmed.aao0475 10.1038/s41422-019-0160-9 10.1371/journal.pone.0050973 10.1073/pnas.0908428107 10.26508/lsa.202000880 10.1002/jbio.200910062 10.1038/s41467-018-05220-6 10.1016/j.trecan.2016.03.001 10.1053/j.gastro.2010.03.034 10.1126/scitranslmed.aai8504 10.1038/s41568-018-0038-z 10.1074/mcp.M116.058313 10.21769/BioProtoc.2265 10.1038/s41598-017-17204-5 10.1016/j.cub.2010.04.042 10.1242/jcs.046870 10.1093/jnci/dju413 10.1073/pnas.1008322108 10.7554/eLife.65234 10.1038/nm.4123 10.1038/s41571-018-0007-1 10.1038/s41556-020-0523-y 10.1021/acsami.5b09344 10.1038/ncomms1414 10.1016/j.ccr.2012.03.002 10.1016/j.ccr.2014.04.005 10.1136/gutjnl-2018-317424 10.1002/pros.23476 10.1016/j.molcel.2013.05.002 10.1126/sciadv.aba6505 10.1016/j.cell.2007.12.033 10.1016/j.ccr.2005.04.023 10.1088/1758-5090/ab5d3f 10.1038/s41556-018-0131-2 10.15252/emmm.201404827 10.1126/scitranslmed.3008639 10.1101/gad.1054603 10.1038/nrc3792 10.1053/j.gastro.2017.11.280 10.1002/emmm.201000106 10.1016/j.cell.2010.03.015 10.1038/sj.onc.1204460 10.1371/journal.pone.0070687 10.1038/nm.4082 10.15252/embr.201439246 10.1038/s41568-020-00329-7 10.1200/JCO.2011.38.9346 10.1016/j.ceb.2019.03.015 10.1038/nprot.2014.024 10.1038/s41591-019-0407-5 10.1016/j.cub.2009.07.069 10.1002/adfm.201504204 10.1016/j.ccell.2015.03.008 10.1038/nrc.2017.41 10.1038/s41416-019-0452-3 10.1158/0008-5472.CAN-10-1855 10.1016/j.pharmthera.2014.10.001 10.1038/nmeth.3839 10.1136/gutjnl-2017-315144 10.1096/fj.201800841R 10.1038/s41467-020-15104-3 10.1093/annonc/mdw427 10.1016/j.celrep.2015.12.020 10.1038/s41467-019-10968-6 10.1016/j.cell.2009.10.027 10.1016/j.devcel.2019.09.018 10.7554/eLife.35800 10.3322/caac.21590 10.1097/01.bcr.0000192266.14329.7b 10.1093/bioinformatics/btv686 10.1038/nature11547 10.1016/j.cell.2015.09.001 |
| ContentType | Journal Article |
| Copyright | Distributed under a Creative Commons Attribution 4.0 International License Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). 2021 The Authors |
| Copyright_xml | – notice: Distributed under a Creative Commons Attribution 4.0 International License – notice: Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). 2021 The Authors |
| CorporateAuthor | Australian Pancreatic Genome Initiative (APGI) Australian Pancreatic Cancer Matrix Atlas (APMA) |
| CorporateAuthor_xml | – name: Australian Pancreatic Cancer Matrix Atlas (APMA) – name: Australian Pancreatic Genome Initiative (APGI) |
| DBID | AAYXX CITATION NPM 7X8 1XC VOOES 5PM |
| DOI | 10.1126/sciadv.abh0363 |
| DatabaseName | CrossRef PubMed MEDLINE - Academic Hyper Article en Ligne (HAL) Hyper Article en Ligne (HAL) (Open Access) PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitleList | PubMed MEDLINE - Academic CrossRef |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) Computer Science |
| EISSN | 2375-2548 |
| ExternalDocumentID | PMC8480933 oai:HAL:hal-03957015v1 34586840 10_1126_sciadv_abh0363 |
| Genre | Journal Article |
| GrantInformation_xml | – fundername: NIGMS NIH HHS grantid: R35 GM140929 – fundername: Cancer Research UK grantid: 29996 – fundername: Cancer Research UK grantid: 21139 – fundername: NIBIB NIH HHS grantid: R01 EB029122 – fundername: ; – fundername: ; grantid: FAKIR 648892 C |
| GroupedDBID | 53G 5VS AAFWJ AAYXX ACGFS ADAXU ADBBV ADPDF ADRAZ AENVI AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS BCGUY BCNDV BKF CITATION EBS EJD FRP GROUPED_DOAJ GX1 HYE KQ8 M48 M~E OK1 OVD OVEED RHI RPM TEORI NPM 7X8 1XC VOOES 5PM |
| ID | FETCH-LOGICAL-c424t-ff8a400d6c00ec3d8dd65dc7d034ece166196fdc43953385a31cb5a5aa33b09d3 |
| ISICitedReferencesCount | 35 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000703091100012&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 2375-2548 |
| IngestDate | Tue Sep 30 16:40:36 EDT 2025 Tue Oct 14 20:47:00 EDT 2025 Thu Jul 10 21:17:05 EDT 2025 Mon Jul 21 06:03:13 EDT 2025 Sat Nov 29 04:57:31 EST 2025 Tue Nov 18 21:46:31 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 40 |
| Language | English |
| License | Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c424t-ff8a400d6c00ec3d8dd65dc7d034ece166196fdc43953385a31cb5a5aa33b09d3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC8480933 These authors contributed equally to this work. |
| ORCID | 0000-0001-7654-9137 0000-0003-1392-3472 0000-0002-5739-8027 0000-0002-9514-7501 0000-0003-0265-326X 0000-0001-5766-9141 0000-0003-4536-9226 0000-0002-3871-3829 0000-0001-9599-9280 0000-0002-4275-4199 0000-0003-1956-0852 0000-0001-5224-0784 0000-0002-0362-5597 0000-0003-3787-277X 0000-0003-3513-1214 0000-0002-5253-7147 0000-0002-3736-7725 0000-0001-9234-0632 0000-0002-0701-8321 0000-0001-9212-6927 0000-0001-9294-1745 0000-0002-1861-1390 0000-0002-4198-3520 0000-0002-3234-3812 0000-0002-5514-7080 0000-0001-9540-3010 0000-0001-7880-6379 0000-0002-7706-5895 0000-0002-3396-846X 0000-0002-4835-4663 0000-0003-2842-8116 0000-0002-8102-7998 0000-0002-4979-9349 0000-0002-9447-1967 |
| OpenAccessLink | http://dx.doi.org/10.1126/sciadv.abh0363 |
| PMID | 34586840 |
| PQID | 2578149858 |
| PQPubID | 23479 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_8480933 hal_primary_oai_HAL_hal_03957015v1 proquest_miscellaneous_2578149858 pubmed_primary_34586840 crossref_citationtrail_10_1126_sciadv_abh0363 crossref_primary_10_1126_sciadv_abh0363 |
| PublicationCentury | 2000 |
| PublicationDate | 2021-10-01 |
| PublicationDateYYYYMMDD | 2021-10-01 |
| PublicationDate_xml | – month: 10 year: 2021 text: 2021-10-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Science advances |
| PublicationTitleAlternate | Sci Adv |
| PublicationYear | 2021 |
| Publisher | American Association for the Advancement of Science (AAAS) American Association for the Advancement of Science |
| Publisher_xml | – name: American Association for the Advancement of Science (AAAS) – name: American Association for the Advancement of Science |
| References | e_1_3_3_50_2 e_1_3_3_75_2 e_1_3_3_71_2 e_1_3_3_77_2 e_1_3_3_79_2 e_1_3_3_16_2 e_1_3_3_18_2 e_1_3_3_39_2 e_1_3_3_12_2 e_1_3_3_37_2 e_1_3_3_58_2 e_1_3_3_14_2 e_1_3_3_35_2 e_1_3_3_56_2 e_1_3_3_33_2 e_1_3_3_54_2 e_1_3_3_10_2 e_1_3_3_31_2 e_1_3_3_52_2 e_1_3_3_73_2 e_1_3_3_40_2 e_1_3_3_61_2 e_1_3_3_5_2 e_1_3_3_7_2 e_1_3_3_9_2 e_1_3_3_27_2 e_1_3_3_29_2 e_1_3_3_23_2 e_1_3_3_48_2 e_1_3_3_69_2 e_1_3_3_25_2 e_1_3_3_46_2 e_1_3_3_67_2 e_1_3_3_80_2 e_1_3_3_44_2 e_1_3_3_65_2 e_1_3_3_3_2 e_1_3_3_21_2 e_1_3_3_42_2 e_1_3_3_51_2 e_1_3_3_74_2 e_1_3_3_76_2 e_1_3_3_70_2 e_1_3_3_78_2 Follain G. (e_1_3_3_63_2) 2019; 2 e_1_3_3_17_2 e_1_3_3_19_2 e_1_3_3_38_2 e_1_3_3_13_2 e_1_3_3_36_2 e_1_3_3_59_2 e_1_3_3_15_2 e_1_3_3_34_2 e_1_3_3_57_2 e_1_3_3_32_2 e_1_3_3_55_2 e_1_3_3_11_2 e_1_3_3_30_2 e_1_3_3_53_2 e_1_3_3_72_2 e_1_3_3_62_2 e_1_3_3_60_2 e_1_3_3_6_2 e_1_3_3_8_2 e_1_3_3_28_2 e_1_3_3_49_2 e_1_3_3_24_2 e_1_3_3_47_2 e_1_3_3_26_2 e_1_3_3_45_2 e_1_3_3_68_2 e_1_3_3_2_2 e_1_3_3_20_2 e_1_3_3_43_2 e_1_3_3_66_2 e_1_3_3_81_2 e_1_3_3_4_2 e_1_3_3_22_2 e_1_3_3_41_2 e_1_3_3_64_2 |
| References_xml | – ident: e_1_3_3_31_2 doi: 10.1038/nature14169 – ident: e_1_3_3_41_2 doi: 10.1038/ncb2775 – ident: e_1_3_3_5_2 doi: 10.1136/gutjnl-2018-316451 – ident: e_1_3_3_37_2 doi: 10.1126/science.1064829 – ident: e_1_3_3_53_2 doi: 10.1126/science.1171362 – ident: e_1_3_3_11_2 doi: 10.15252/emmm.202012010 – ident: e_1_3_3_42_2 doi: 10.1038/ncb2548 – ident: e_1_3_3_12_2 doi: 10.1126/scitranslmed.aao0475 – ident: e_1_3_3_73_2 doi: 10.1038/s41422-019-0160-9 – ident: e_1_3_3_67_2 doi: 10.1371/journal.pone.0050973 – ident: e_1_3_3_28_2 doi: 10.1073/pnas.0908428107 – ident: e_1_3_3_47_2 doi: 10.26508/lsa.202000880 – ident: e_1_3_3_48_2 doi: 10.1002/jbio.200910062 – ident: e_1_3_3_56_2 doi: 10.1038/s41467-018-05220-6 – ident: e_1_3_3_24_2 doi: 10.1016/j.trecan.2016.03.001 – ident: e_1_3_3_30_2 doi: 10.1053/j.gastro.2010.03.034 – ident: e_1_3_3_19_2 doi: 10.1126/scitranslmed.aai8504 – ident: e_1_3_3_15_2 doi: 10.1038/s41568-018-0038-z – ident: e_1_3_3_68_2 doi: 10.1074/mcp.M116.058313 – ident: e_1_3_3_52_2 doi: 10.21769/BioProtoc.2265 – ident: e_1_3_3_80_2 doi: 10.1038/s41598-017-17204-5 – ident: e_1_3_3_40_2 doi: 10.1016/j.cub.2010.04.042 – ident: e_1_3_3_39_2 doi: 10.1242/jcs.046870 – ident: e_1_3_3_3_2 doi: 10.1093/jnci/dju413 – ident: e_1_3_3_78_2 doi: 10.1073/pnas.1008322108 – ident: e_1_3_3_74_2 doi: 10.7554/eLife.65234 – volume: 2 start-page: 107 year: 2019 ident: e_1_3_3_63_2 article-title: Fluids and their mechanics in tumour transit: Shaping metastasis publication-title: Nat. Rev. Cancer – ident: e_1_3_3_9_2 doi: 10.1038/nm.4123 – ident: e_1_3_3_17_2 doi: 10.1038/s41571-018-0007-1 – ident: e_1_3_3_18_2 doi: 10.1038/s41556-020-0523-y – ident: e_1_3_3_62_2 doi: 10.1021/acsami.5b09344 – ident: e_1_3_3_34_2 doi: 10.1038/ncomms1414 – ident: e_1_3_3_55_2 doi: 10.1016/j.ccr.2012.03.002 – ident: e_1_3_3_8_2 doi: 10.1016/j.ccr.2014.04.005 – ident: e_1_3_3_54_2 doi: 10.1136/gutjnl-2018-317424 – ident: e_1_3_3_69_2 doi: 10.1002/pros.23476 – ident: e_1_3_3_49_2 doi: 10.1016/j.molcel.2013.05.002 – ident: e_1_3_3_46_2 doi: 10.1126/sciadv.aba6505 – ident: e_1_3_3_35_2 doi: 10.1016/j.cell.2007.12.033 – ident: e_1_3_3_36_2 doi: 10.1016/j.ccr.2005.04.023 – ident: e_1_3_3_60_2 doi: 10.1088/1758-5090/ab5d3f – ident: e_1_3_3_6_2 doi: 10.1038/s41556-018-0131-2 – ident: e_1_3_3_7_2 doi: 10.15252/emmm.201404827 – ident: e_1_3_3_21_2 doi: 10.1126/scitranslmed.3008639 – ident: e_1_3_3_23_2 doi: 10.1101/gad.1054603 – ident: e_1_3_3_14_2 doi: 10.1038/nrc3792 – ident: e_1_3_3_4_2 doi: 10.1053/j.gastro.2017.11.280 – ident: e_1_3_3_65_2 doi: 10.1002/emmm.201000106 – ident: e_1_3_3_76_2 doi: 10.1016/j.cell.2010.03.015 – ident: e_1_3_3_77_2 doi: 10.1038/sj.onc.1204460 – ident: e_1_3_3_81_2 doi: 10.1371/journal.pone.0070687 – ident: e_1_3_3_10_2 doi: 10.1038/nm.4082 – ident: e_1_3_3_44_2 doi: 10.15252/embr.201439246 – ident: e_1_3_3_75_2 doi: 10.1038/s41568-020-00329-7 – ident: e_1_3_3_38_2 doi: 10.1200/JCO.2011.38.9346 – ident: e_1_3_3_26_2 doi: 10.1016/j.ceb.2019.03.015 – ident: e_1_3_3_43_2 doi: 10.1038/nprot.2014.024 – ident: e_1_3_3_20_2 doi: 10.1038/s41591-019-0407-5 – ident: e_1_3_3_58_2 doi: 10.1016/j.cub.2009.07.069 – ident: e_1_3_3_59_2 doi: 10.1002/adfm.201504204 – ident: e_1_3_3_16_2 doi: 10.1016/j.ccell.2015.03.008 – ident: e_1_3_3_25_2 doi: 10.1038/nrc.2017.41 – ident: e_1_3_3_70_2 doi: 10.1038/s41416-019-0452-3 – ident: e_1_3_3_57_2 doi: 10.1158/0008-5472.CAN-10-1855 – ident: e_1_3_3_13_2 doi: 10.1016/j.pharmthera.2014.10.001 – ident: e_1_3_3_61_2 doi: 10.1038/nmeth.3839 – ident: e_1_3_3_32_2 doi: 10.1136/gutjnl-2017-315144 – ident: e_1_3_3_66_2 doi: 10.1096/fj.201800841R – ident: e_1_3_3_72_2 doi: 10.1038/s41467-020-15104-3 – ident: e_1_3_3_22_2 doi: 10.1093/annonc/mdw427 – ident: e_1_3_3_29_2 doi: 10.1016/j.celrep.2015.12.020 – ident: e_1_3_3_27_2 doi: 10.1038/s41467-019-10968-6 – ident: e_1_3_3_45_2 doi: 10.1016/j.cell.2009.10.027 – ident: e_1_3_3_50_2 doi: 10.1016/j.devcel.2019.09.018 – ident: e_1_3_3_64_2 doi: 10.7554/eLife.35800 – ident: e_1_3_3_2_2 doi: 10.3322/caac.21590 – ident: e_1_3_3_51_2 doi: 10.1097/01.bcr.0000192266.14329.7b – ident: e_1_3_3_79_2 doi: 10.1093/bioinformatics/btv686 – ident: e_1_3_3_33_2 doi: 10.1038/nature11547 – ident: e_1_3_3_71_2 doi: 10.1016/j.cell.2015.09.001 |
| SSID | ssj0001466519 |
| Score | 2.3456423 |
| Snippet | Intravital imaging guides a personalized medicine approach to target mechanoreciprocity in pancreatic cancer.
Pancreatic ductal adenocarcinoma (PDAC) is a... Pancreatic ductal adenocarcinoma (PDAC) is a highly metastatic, chemoresistant malignancy and is characterized by a dense, desmoplastic stroma that modulates... Intravital imaging guides a personalized medicine approach to target mechanoreciprocity in pancreatic cancer. Pancreatic ductal adenocarcinoma (PDAC) is a... |
| SourceID | pubmedcentral hal proquest pubmed crossref |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | eabh0363 |
| SubjectTerms | Biomedicine and Life Sciences Cancer Cell Biology Computer Science Medical Imaging SciAdv r-articles |
| Title | Intravital imaging technology guides FAK-mediated priming in pancreatic cancer precision medicine according to Merlin status |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/34586840 https://www.proquest.com/docview/2578149858 https://hal.science/hal-03957015 https://pubmed.ncbi.nlm.nih.gov/PMC8480933 |
| Volume | 7 |
| WOSCitedRecordID | wos000703091100012&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2375-2548 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001466519 issn: 2375-2548 databaseCode: DOA dateStart: 20150101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2375-2548 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001466519 issn: 2375-2548 databaseCode: M~E dateStart: 20150101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1bb9MwFLbawQMviHHtgMogxEVRUBI7ifNYTauG1hU0dahvkeM4tFKXlt4YEuLf8D85jp1L2ZDGAy9R5VpO2u_L8TnH54LQq4SEACzx7ciH141KmtmcBdxOkiiLCOWB9ETRbCIcDtl4HH1qtX6VuTDbWZjn7PIyWvxXqGEMwFaps_8Ad7UoDMBnAB2uADtcbwT8B-Wu3apeINb0QvcgWlf-c-vLZprKldXvndhF0ohSOBdFa68itQVkg1YjhQoHE3KpigjoLjzVMbzFhTJZi4Xn1qmul6USk4wPwai6pdQwUQarq9CeKPf7bCbro6kzKdM68b32s35WMTg6PGDGG_G6h_P8G_9eRftaZzpesHRjeG4VEGeknUdC3wZrVQtjec2YEddhg5W60pORvZInE3Usff3GULayhB_9vjFxtwL38GPcPx8M4tHRePR68dVWzcnUIb7p1NJGt7zQj5TwPP3ZcOXRIPCLFjLVA5vyoCY9q3HPHfWnPVHBt1ctmz8DdBsaz-geumtMFdzTFNtHLZnfR_sG1hV-ayqWv3uAftScw4ZzuOYc1pzDTc5hwzk8zXHNOaw5hyvO4ZJzuOIcXs-x5hzWnHuIzvtHo8Nj27T1sAX16NrOMsZh50gD4ThSkJSlaeCnIkwdQqWQLmiMUZClAlRlsEWYz4krEp_7nBOSOFFKHqG9fJ7LJwgTR4AGmnFHgB4dZRKWylxXhpRJkVFPdJBd_tuxMDXvVeuVWVzYvl4Qa3Rig04HvanmL3S1l7_OfAngVZNUkfbj3iBWY446-gYte-t20IsS2xiktjqK47mcb1ax2ihdGjGfddBjjXW1FqE-UyWYOijcYcHOzXa_yaeTojI8o0x5KA9ucN-n6E79Gj5De-vlRj5Ht8V2PV0tu6gdjlm3cFB1C7L_Bp0l4UQ |
| linkProvider | ISSN International Centre |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Intravital+imaging+technology+guides+FAK-mediated+priming+in+pancreatic+cancer+precision+medicine+according+to+Merlin+status&rft.jtitle=Science+advances&rft.au=Murphy%2C+Kendelle+J&rft.au=Reed%2C+Daniel+A&rft.au=Vennin%2C+Claire&rft.au=Conway%2C+James+R+W&rft.date=2021-10-01&rft.issn=2375-2548&rft.eissn=2375-2548&rft.volume=7&rft.issue=40&rft.spage=eabh0363&rft_id=info:doi/10.1126%2Fsciadv.abh0363&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2375-2548&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2375-2548&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2375-2548&client=summon |