Ephedra alata Seeds Confer Kidney Protection against Early Life Exposure to Acephate by Regulating Oxidative Insult and Activating Autophagy

The aim of the current work was to examine for the first time the nephropreventive capacity of Ephedra alata seed extract (E) against maternal exposure to acephate in rat offspring. The in vivo results revealed that E. alata supplementation for 28 days (40 mg/kg b.w.) significantly attenuated the ne...

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Vydáno v:Life (Basel, Switzerland) Ročník 13; číslo 12; s. 2254
Hlavní autoři: Mufti, Afoua, Feriani, Anouar, Contreras, María del Mar, Nehdi, Saber, Hfaeidh, Najla, Tlili, Nizar, Harrath, Abdel Halim
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland MDPI AG 25.11.2023
Témata:
acephate
Antioxidants
Autophagy
Bioactive compounds
Biomarkers
Creatinine
DNA fragmentation
Drug dosages
Ephedra
Ephedra alata
Exposure
Fatty acids
Gene expression
Genes
Immunofluorescence
Kidney diseases
Kidneys
Laboratory animals
Lipid peroxidation
Lipids
Molecular docking
Na+/H+-exchanging ATPase
Na+/K+-exchanging ATPase
nephrotoxicity
Offspring
Oxidative stress
Peroxidation
Pesticides
Phytochemicals
Proteins
Risk factors
Seeds
Sodium
Stress analysis
Uric acid
United States > US
Tunisia
acephate
Ephedra alata
autophagy
molecular docking
nephrotoxicity
oxidative stress
ISSN:2075-1729, 2075-1729
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Shrnutí:The aim of the current work was to examine for the first time the nephropreventive capacity of Ephedra alata seed extract (E) against maternal exposure to acephate in rat offspring. The in vivo results revealed that E. alata supplementation for 28 days (40 mg/kg b.w.) significantly attenuated the nephrotoxicity in adult offspring induced by acephate. In fact, it decreased the levels of creatinine and uric acid and increased the albumin content compared to the intoxicated group. The in utero studies showed that E. alata inhibited the renal oxidative stress generated by acephate exposure by reducing lipid peroxidation and enhancing antioxidant biomarker activities (GSH, CAT, and SOD). The inhibition of DNA fragmentation and the improvement of the ultrastructural changes highlighted the prophylactic effect of E. alata in renal tissue. Additionally, the immunofluorescence study showed the upregulation of LC3 gene expression, suggesting the capacity of E. alata extract to stimulate autophagic processes as a protective mechanism. Molecular docking analysis indicated that hexadecasphinganine, the major compound in E. alata, has a higher affinity toward the Na+/K+-ATPase, epithelial sodium channel (ENaC), and sodium hydrogen exchanger 3 (NHE3) genes than acephate. Hexadecasphinganine could be considered a potential inhibitor of the activity of these genes and therefore exerted its preventive capacity. The obtained findings confirmed that E. alata seed extract exerted nephropreventive capacities, which could be related to its bioactive compounds, which possess antioxidant activities.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:2075-1729
2075-1729
DOI:10.3390/life13122254