Activation of human natural killer cells by lipopolysaccharide from Actinobacillus actinomycetemcomitans

Lipopolysaccharide (LPS) from the Y4 strain of this bacterium, which is implicated in the pathogenesis of juvenile periodontitis, was incubated with human peripheral blood lymphocytes (PBL) and its action compared to that of LPS from Escherichia coli. Both LPS augmented cytotoxicity measured against...

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Bibliographic Details
Published in:Archives of oral biology Vol. 34; no. 6; p. 459
Main Authors: Lindemann, R A, Eilber, F
Format: Journal Article
Language:English
Published: England 1989
Subjects:
Actinobacillus - immunology
Cells, Cultured
Cytotoxicity Tests, Immunologic
DNA - biosynthesis
Humans
Interleukin-2 - immunology
Killer Cells, Natural - immunology
Lipopolysaccharides - immunology
Lymphocyte Activation
Tumor Cells, Cultured
ISSN:0003-9969
Online Access:Get more information
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Summary:Lipopolysaccharide (LPS) from the Y4 strain of this bacterium, which is implicated in the pathogenesis of juvenile periodontitis, was incubated with human peripheral blood lymphocytes (PBL) and its action compared to that of LPS from Escherichia coli. Both LPS augmented cytotoxicity measured against natural killer (NK) cell-resistant tumour targets within 24 h of incubation. Cytotoxicity was exclusively found in NK-enriched low-density large granular lymphocyte fractions, as separated by Percoll gradient. LPS activated NK cells without stimulating high levels of proliferation. The minimum concentration of A. actinomycetemcomitans LPS required to activate NK cells was 1 microgram/ml; higher concentrations did not significantly increase this activation. LPS had no synergistic effect on the induction of PBL cytotoxicity by interleukin-2. In contrast, LPS pre-activated monocytes inhibited the induction of lymphocyte cytotoxicity by either interleukin-2 or LPS.
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ISSN:0003-9969
DOI:10.1016/0003-9969(89)90125-8