Rapid method for the quantification of amoxicillin and its major metabolites in pig tissues by liquid chromatography-tandem mass spectrometry with emphasis on stability issues

A fast method for the quantitative determination of amoxicillin (AMO), amoxicilloic acid (AMA) and amoxicillin diketopiperazine-2′,5′-dione (DIKETO) in pig edible tissues (kidney, liver, fat and muscle) with liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) is presented. The method uses...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 861; no. 1; pp. 108 - 116
Main Authors: Reyns, Tim, Cherlet, Marc, De Baere, Siegrid, De Backer, Patrick, Croubels, Siska
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 2008
Elsevier Science
Subjects:
Amoxicillin
Amoxicillin - analysis
Amoxicillin - chemistry
Amoxicillin - metabolism
Amoxicillin diketopiperazine-2',5'-dione
Amoxicilloic acid
Analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Chromatography, Liquid - methods
electrospray ionization-tandem mass spectrometry
Fundamental and applied biological sciences. Psychology
General pharmacology
Liquid chromatography
Liquid chromatography/electrospray ionization-tandem mass spectrometry
Medical sciences
Molecular Structure
Pharmacology. Drug treatments
Pig tissues
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization - methods
Stability
Swine
Tandem Mass Spectrometry - methods
Amoxicilloic acid
Amoxicillin diketopiperazine-2′,5′-dione
Liquid chromatography/electrospray ionization-tandem mass spectrometry
Stability
Pig tissues
Amoxicillin
Metabolite
Cyclic peptides
Electrospray
Penicillin derivatives
Tissue
Rapid technique
Pia tissues Stability
Ungulata
Quantitative analysis
Amoxicillin diketopiperazine-2',5'-dione
β-Lactams
Pig
Vertebrata
Antibiotic
Mammalia
Animal
Mass spectrometry MS/MS
Dipeptides
Artiodactyla
Antibacterial agent
Physicochemical properties
ISSN:1570-0232, 1873-376X
Online Access:Get full text
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Summary:A fast method for the quantitative determination of amoxicillin (AMO), amoxicilloic acid (AMA) and amoxicillin diketopiperazine-2′,5′-dione (DIKETO) in pig edible tissues (kidney, liver, fat and muscle) with liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) is presented. The method uses a simple liquid–liquid extraction of the tissue matrix with a 10 mM potassium dihydrogen phosphate buffer (pH 4.5) as extraction solvent. After deproteinisation by ultrafiltration, the tissue extract was directly injected onto the LC column. Chromatographic separation of the components was performed on a PLRP-S polymeric column using 0.1% of formic acid in water and acetonitrile. The mass spectrometer was operated in the positive electrospray MS/MS mode. The method was fully validated according to EU requirements (linearity, precision, trueness, quantification limit, detection limit and specificity). The stability of the components was evaluated over the pH range from 1.2 to 8.0. Biological samples of pigs medicated with AMO and AMO/clavulanic acid were analyzed using the developed method.
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content type line 23
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2007.11.007