Promotion of Germination Using Hydroxamic Acid Inhibitors of 9-cis-Epoxycarotenoid Dioxygenase

Abscisic acid (ABA) inhibits seed germination and the regulation of ABA biosynthesis has a role in maintenance of seed dormancy. The key rate-limiting step in ABA biosynthesis is catalyzed by 9- -epoxycarotenoid dioxygenase (NCED). Two hydroxamic acid inhibitors of carotenoid cleavage dioxygenase (C...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Frontiers in plant science Ročník 8; s. 357
Hlavní autoři: Awan, Sajjad Z., Chandler, Jake O., Harrison, Peter J., Sergeant, Martin J., Bugg, Timothy D. H., Thompson, Andrew J.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland Frontiers Media SA 20.03.2017
Frontiers Media S.A
Témata:
Abscisic acid
Accumulation
Biosynthesis
Carotenoids
Dioxygenase
Dormancy
Gene expression
Germination
Herbicides
Hydroxamic acid
Inhibitors
Lead compounds
Mode of action
Osmolarity
Plant Science
Polyethylene glycol
Seed germination
Seedlings
Seeds
Tobacco
Tomatoes
Transgenes
abscisic acid
hydroxamic acid
seed germination
chemical genetics
9-cis-epoxycarotenoid dioxygenase
dormancy
ISSN:1664-462X, 1664-462X
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:Abscisic acid (ABA) inhibits seed germination and the regulation of ABA biosynthesis has a role in maintenance of seed dormancy. The key rate-limiting step in ABA biosynthesis is catalyzed by 9- -epoxycarotenoid dioxygenase (NCED). Two hydroxamic acid inhibitors of carotenoid cleavage dioxygenase (CCD), D4 and D7, previously found to inhibit CCD and NCED , are shown to have the novel property of decreasing mean germination time of tomato ( L.) seeds constitutively overexpressing . Post-germination, D4 exhibited no negative effects on tomato seedling growth in terms of height, dry weight, and fresh weight. Tobacco ( L.) seeds containing a tetracycline-inducible transgene were used to show that germination could be negatively and positively controlled through the chemical induction of gene expression and the chemical inhibition of the NCED protein: application of tetracycline increased mean germination time and delayed hypocotyl emergence in a similar manner to that observed when exogenous ABA was applied and this was reversed by D4 when expression was induced at intermediate levels. D4 also improved germination in lettuce ( L.) seeds under thermoinhibitory temperatures and in tomato seeds imbibed in high osmolarity solutions of polyethylene glycol. D4 reduced ABA and dihydrophaseic acid accumulation in tomato seeds overexpressing and reduced ABA accumulation in wild type tomato seeds imbibed on polyethylene glycol. The evidence supports a mode of action of D4 through NCED inhibition, and this molecule provides a lead compound for the design of NCED inhibitors with greater specificity and potency.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science
Peter J. Harrison, School of Chemistry, University of Edinburgh, Edinburgh, UK
Edited by: Qi Xie, Chinese Academy of Sciences, China
Present Address: Sajjad Z. Awan, Agriculture and Horticulture Development Board (Cereals and Oilseeds), Coventry, UK
Reviewed by: Gang Li, University of Nebraka-Lincoln, USA; Jorge E. Mayer, Ag RD&IP Consult P/L, Australia
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2017.00357