Fatty Acid Uptake and Lipid Storage Induced by HIF-1α Contribute to Cell Growth and Survival after Hypoxia-Reoxygenation

An in vivo model of antiangiogenic therapy allowed us to identify genes upregulated by bevacizumab treatment, including Fatty Acid Binding Protein 3 (FABP3) and FABP7, both of which are involved in fatty acid uptake. In vitro, both were induced by hypoxia in a hypoxia-inducible factor-1α (HIF-1α)-de...

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Veröffentlicht in:Cell reports (Cambridge) Jg. 9; H. 1; S. 349 - 365
Hauptverfasser: Bensaad, Karim, Favaro, Elena, Lewis, Caroline A., Peck, Barrie, Lord, Simon, Collins, Jennifer M., Pinnick, Katherine E., Wigfield, Simon, Buffa, Francesca M., Li, Ji-Liang, Zhang, Qifeng, Wakelam, Michael J.O., Karpe, Fredrik, Schulze, Almut, Harris, Adrian L.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Elsevier 09.10.2014
Schlagworte:
Cell Hypoxia - physiology
Cell Line, Tumor
Cell Proliferation - physiology
Fatty Acids - metabolism
Glioblastoma
Humans
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Lipid Metabolism
Oxidation-Reduction
Oxygen - metabolism
ISSN:2211-1247, 2211-1247
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Zusammenfassung:An in vivo model of antiangiogenic therapy allowed us to identify genes upregulated by bevacizumab treatment, including Fatty Acid Binding Protein 3 (FABP3) and FABP7, both of which are involved in fatty acid uptake. In vitro, both were induced by hypoxia in a hypoxia-inducible factor-1α (HIF-1α)-dependent manner. There was a significant lipid droplet (LD) accumulation in hypoxia that was time and O2 concentration dependent. Knockdown of endogenous expression of FABP3, FABP7, or Adipophilin (an essential LD structural component) significantly impaired LD formation under hypoxia. We showed that LD accumulation is due to FABP3/7-dependent fatty acid uptake while de novo fatty acid synthesis is repressed in hypoxia. We also showed that ATP production occurs via β-oxidation or glycogen degradation in a cell-type-dependent manner in hypoxia-reoxygenation. Finally, inhibition of lipid storage reduced protection against reactive oxygen species toxicity, decreased the survival of cells subjected to hypoxia-reoxygenation in vitro, and strongly impaired tumorigenesis in vivo.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2014.08.056