microRNAs in action: biogenesis, function and regulation

Ever since microRNAs (miRNAs) were first recognized as an extensive gene family >20 years ago, a broad community of researchers was drawn to investigate the universe of small regulatory RNAs. Although core features of miRNA biogenesis and function were revealed early on, recent years continue to...

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Veröffentlicht in:Nature reviews. Genetics Jg. 24; H. 12; S. 816 - 833
Hauptverfasser: Shang, Renfu, Lee, Seungjae, Senavirathne, Gayan, Lai, Eric C
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Nature Publishing Group 01.12.2023
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ISSN:1471-0056, 1471-0064, 1471-0064
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Abstract Ever since microRNAs (miRNAs) were first recognized as an extensive gene family >20 years ago, a broad community of researchers was drawn to investigate the universe of small regulatory RNAs. Although core features of miRNA biogenesis and function were revealed early on, recent years continue to uncover fundamental information on the structural and molecular dynamics of core miRNA machinery, how miRNA substrates and targets are selected from the transcriptome, new avenues for multilevel regulation of miRNA biogenesis and mechanisms for miRNA turnover. Many of these latest insights were enabled by recent technological advances, including massively parallel assays, cryogenic electron microscopy, single-molecule imaging and CRISPR-Cas9 screening. Here, we summarize the current understanding of miRNA biogenesis, function and regulation, and outline challenges to address in the future.
AbstractList Ever since microRNAs (miRNAs) were first recognized as an extensive gene family >20 years ago, a broad community of researchers was drawn to investigate the universe of small regulatory RNAs. Although core features of miRNA biogenesis and function were revealed early on, recent years continue to uncover fundamental information on the structural and molecular dynamics of core miRNA machinery, how miRNA substrates and targets are selected from the transcriptome, new avenues for multilevel regulation of miRNA biogenesis and mechanisms for miRNA turnover. Many of these latest insights were enabled by recent technological advances, including massively parallel assays, cryogenic electron microscopy, single-molecule imaging and CRISPR–Cas9 screening. Here, we summarize the current understanding of miRNA biogenesis, function and regulation, and outline challenges to address in the future.In this Review, the authors describe how the application of new technologies to the microRNA (miRNA) field has yielded key insights into miRNA biology. The authors summarize our current understanding of miRNA biogenesis, function and processing, and highlight challenges to address in future research.
Ever since microRNAs (miRNAs) were first recognized as an extensive gene family >20 years ago, a broad community of researchers was drawn to investigate the universe of small regulatory RNAs. Although core features of miRNA biogenesis and function were revealed early on, recent years continue to uncover fundamental information on the structural and molecular dynamics of core miRNA machinery, how miRNA substrates and targets are selected from the transcriptome, new avenues for multilevel regulation of miRNA biogenesis and mechanisms for miRNA turnover. Many of these latest insights were enabled by recent technological advances, including massively parallel assays, cryogenic electron microscopy, single-molecule imaging and CRISPR-Cas9 screening. Here, we summarize the current understanding of miRNA biogenesis, function and regulation, and outline challenges to address in the future.
Ever since microRNAs (miRNAs) were first recognized as an extensive gene family >20 years ago, a broad community of researchers was drawn to investigate the universe of small regulatory RNAs. Although core features of miRNA biogenesis and function were revealed early on, recent years continue to uncover fundamental information on the structural and molecular dynamics of core miRNA machinery, how miRNA substrates and targets are selected from the transcriptome, new avenues for multilevel regulation of miRNA biogenesis and mechanisms for miRNA turnover. Many of these latest insights were enabled by recent technological advances, including massively parallel assays, cryogenic electron microscopy, single-molecule imaging and CRISPR-Cas9 screening. Here, we summarize the current understanding of miRNA biogenesis, function and regulation, and outline challenges to address in the future.Ever since microRNAs (miRNAs) were first recognized as an extensive gene family >20 years ago, a broad community of researchers was drawn to investigate the universe of small regulatory RNAs. Although core features of miRNA biogenesis and function were revealed early on, recent years continue to uncover fundamental information on the structural and molecular dynamics of core miRNA machinery, how miRNA substrates and targets are selected from the transcriptome, new avenues for multilevel regulation of miRNA biogenesis and mechanisms for miRNA turnover. Many of these latest insights were enabled by recent technological advances, including massively parallel assays, cryogenic electron microscopy, single-molecule imaging and CRISPR-Cas9 screening. Here, we summarize the current understanding of miRNA biogenesis, function and regulation, and outline challenges to address in the future.
Author Lai, Eric C
Lee, Seungjae
Senavirathne, Gayan
Shang, Renfu
Author_xml – sequence: 1
  givenname: Renfu
  surname: Shang
  fullname: Shang, Renfu
  organization: Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA
– sequence: 2
  givenname: Seungjae
  orcidid: 0000-0001-5626-9656
  surname: Lee
  fullname: Lee, Seungjae
  organization: Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA
– sequence: 3
  givenname: Gayan
  surname: Senavirathne
  fullname: Senavirathne, Gayan
  organization: Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA
– sequence: 4
  givenname: Eric C
  orcidid: 0000-0002-8432-5851
  surname: Lai
  fullname: Lai, Eric C
  email: laie@mskcc.org
  organization: Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA. laie@mskcc.org
BackLink https://www.ncbi.nlm.nih.gov/pubmed/37380761$$D View this record in MEDLINE/PubMed
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Snippet Ever since microRNAs (miRNAs) were first recognized as an extensive gene family >20 years ago, a broad community of researchers was drawn to investigate the...
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SubjectTerms Biosynthesis
CRISPR
Electron microscopy
MicroRNAs
MicroRNAs - genetics
miRNA
Transcriptome
Transcriptomes
Title microRNAs in action: biogenesis, function and regulation
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