Improves the In Vitro Developmental Competence and Reprogramming Efficiency of Cloned Bovine Embryos by Additional Complimentary Cytoplasm
Somatic cell nuclear transfer (SCNT) is a useful technology; however, its efficiency is low. In this study, we investigated the effects of cytoplasmic transfer into enucleated oocytes on the developmental competence and quality of cloned preimplantation bovine embryos via terminal deoxynucleotidyl t...
Saved in:
| Published in: | Cellular reprogramming Vol. 21; no. 1; p. 51 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
01.02.2019
|
| Subjects: | |
| ISSN: | 2152-4998, 2152-4998 |
| Online Access: | Get more information |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Somatic cell nuclear transfer (SCNT) is a useful technology; however, its efficiency is low. In this study, we investigated the effects of cytoplasmic transfer into enucleated oocytes on the developmental competence and quality of cloned preimplantation bovine embryos via terminal deoxynucleotidyl transferase dUTP nick-end labeling, quantitative reverse transcription PCR, and immunocytochemistry. We used cytoplasm injection cloning technology (CICT), a new technique via which the cytoplasmic volume of an enucleated oocyte could be restored by injecting ∼30% of the cytoplasm of a donor oocyte. The percentages of embryos that underwent cleavage and formed a blastocyst were significantly higher (p < 0.05) in the CICT group than in the SCNT group (28.9 ± 0.8% vs. 20.2 ± 1.3%, respectively). Furthermore, the total cell number per day 8 blastocyst was significantly higher in the CICT group than in the SCNT group (176.2 ± 6.5 vs. 119.3 ± 7.7, p < 0.05). Moreover, CICT increased mitochondrial activity, as detected using MitoTracker
Green. The mRNA levels of DNA methyltransferase 1 and DNA methyltransferase 3a were significantly lower (p < 0.05) in the CICT group than in the SCNT group. The mRNA level of DNA methyltransferase 3b was lower in the CICT group than in the SCNT group; however, this difference was not significant (p > 0.05). Taken together, these data suggest that CICT improves the in vitro developmental competence and quality of cloned bovine embryos. |
|---|---|
| AbstractList | Somatic cell nuclear transfer (SCNT) is a useful technology; however, its efficiency is low. In this study, we investigated the effects of cytoplasmic transfer into enucleated oocytes on the developmental competence and quality of cloned preimplantation bovine embryos via terminal deoxynucleotidyl transferase dUTP nick-end labeling, quantitative reverse transcription PCR, and immunocytochemistry. We used cytoplasm injection cloning technology (CICT), a new technique via which the cytoplasmic volume of an enucleated oocyte could be restored by injecting ∼30% of the cytoplasm of a donor oocyte. The percentages of embryos that underwent cleavage and formed a blastocyst were significantly higher (p < 0.05) in the CICT group than in the SCNT group (28.9 ± 0.8% vs. 20.2 ± 1.3%, respectively). Furthermore, the total cell number per day 8 blastocyst was significantly higher in the CICT group than in the SCNT group (176.2 ± 6.5 vs. 119.3 ± 7.7, p < 0.05). Moreover, CICT increased mitochondrial activity, as detected using MitoTracker
Green. The mRNA levels of DNA methyltransferase 1 and DNA methyltransferase 3a were significantly lower (p < 0.05) in the CICT group than in the SCNT group. The mRNA level of DNA methyltransferase 3b was lower in the CICT group than in the SCNT group; however, this difference was not significant (p > 0.05). Taken together, these data suggest that CICT improves the in vitro developmental competence and quality of cloned bovine embryos. Somatic cell nuclear transfer (SCNT) is a useful technology; however, its efficiency is low. In this study, we investigated the effects of cytoplasmic transfer into enucleated oocytes on the developmental competence and quality of cloned preimplantation bovine embryos via terminal deoxynucleotidyl transferase dUTP nick-end labeling, quantitative reverse transcription PCR, and immunocytochemistry. We used cytoplasm injection cloning technology (CICT), a new technique via which the cytoplasmic volume of an enucleated oocyte could be restored by injecting ∼30% of the cytoplasm of a donor oocyte. The percentages of embryos that underwent cleavage and formed a blastocyst were significantly higher (p < 0.05) in the CICT group than in the SCNT group (28.9 ± 0.8% vs. 20.2 ± 1.3%, respectively). Furthermore, the total cell number per day 8 blastocyst was significantly higher in the CICT group than in the SCNT group (176.2 ± 6.5 vs. 119.3 ± 7.7, p < 0.05). Moreover, CICT increased mitochondrial activity, as detected using MitoTracker® Green. The mRNA levels of DNA methyltransferase 1 and DNA methyltransferase 3a were significantly lower (p < 0.05) in the CICT group than in the SCNT group. The mRNA level of DNA methyltransferase 3b was lower in the CICT group than in the SCNT group; however, this difference was not significant (p > 0.05). Taken together, these data suggest that CICT improves the in vitro developmental competence and quality of cloned bovine embryos.Somatic cell nuclear transfer (SCNT) is a useful technology; however, its efficiency is low. In this study, we investigated the effects of cytoplasmic transfer into enucleated oocytes on the developmental competence and quality of cloned preimplantation bovine embryos via terminal deoxynucleotidyl transferase dUTP nick-end labeling, quantitative reverse transcription PCR, and immunocytochemistry. We used cytoplasm injection cloning technology (CICT), a new technique via which the cytoplasmic volume of an enucleated oocyte could be restored by injecting ∼30% of the cytoplasm of a donor oocyte. The percentages of embryos that underwent cleavage and formed a blastocyst were significantly higher (p < 0.05) in the CICT group than in the SCNT group (28.9 ± 0.8% vs. 20.2 ± 1.3%, respectively). Furthermore, the total cell number per day 8 blastocyst was significantly higher in the CICT group than in the SCNT group (176.2 ± 6.5 vs. 119.3 ± 7.7, p < 0.05). Moreover, CICT increased mitochondrial activity, as detected using MitoTracker® Green. The mRNA levels of DNA methyltransferase 1 and DNA methyltransferase 3a were significantly lower (p < 0.05) in the CICT group than in the SCNT group. The mRNA level of DNA methyltransferase 3b was lower in the CICT group than in the SCNT group; however, this difference was not significant (p > 0.05). Taken together, these data suggest that CICT improves the in vitro developmental competence and quality of cloned bovine embryos. |
| Author | Mesalam, Ayman Lee, Kyeong-Lim Xu, Lianguang Khan, Imran Song, Seok-Hwan Chowdhury, M M R Lv, Wenfa Kong, Il-Keun |
| Author_xml | – sequence: 1 givenname: Lianguang surname: Xu fullname: Xu, Lianguang organization: 1 Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Republic of Korea – sequence: 2 givenname: Ayman surname: Mesalam fullname: Mesalam, Ayman organization: 2 Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt – sequence: 3 givenname: Kyeong-Lim surname: Lee fullname: Lee, Kyeong-Lim organization: 1 Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Republic of Korea – sequence: 4 givenname: Seok-Hwan surname: Song fullname: Song, Seok-Hwan organization: 1 Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Republic of Korea – sequence: 5 givenname: Imran surname: Khan fullname: Khan, Imran organization: 1 Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Republic of Korea – sequence: 6 givenname: M M R surname: Chowdhury fullname: Chowdhury, M M R organization: 3 Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon, Republic of Korea – sequence: 7 givenname: Wenfa surname: Lv fullname: Lv, Wenfa organization: 4 Division of Animal Reproduction and Breeding, Department of Animal Science, Jilin Agricultural University, Changchun, Republic of China – sequence: 8 givenname: Il-Keun surname: Kong fullname: Kong, Il-Keun organization: 5 Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Republic of Korea |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30735075$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNUE1LxDAUDKL4ffUoOXrZNR-bNj1qXXVhQRD1uiTNi0aapDbdhf4Ff7VRV_Bd3oM3M8zMEdoNMQBCZ5RMKZHVZQNtO2WEyikhguygQ0YFm8yqSu7-uw_QaUrvJA_nmVbsowNOSi5IKQ7R58J3fdxAwsMb4EXAL27oI76BDbSx8xAG1eI6-g4GCA1gFQx-hEx57ZX3LrziubWucfk54mhx3WaLBl_HjQuA5173Y0xYj_jKGDe4GLZyrfvR7kdcj0PsWpX8Cdqzqk1wut3H6Pl2_lTfT5YPd4v6ajlpZrQaJlRRqwUHKbUkFdWFZYJbqRpOTJkRthFacskrUWqtCgtEzWaVKYwxzFAm2DG6-NXNKT7WkIaVd-m7ShUgrtOKUSlEIWRZZuj5FrrWHsyq653Pnld__bEvwXJ3YA |
| CitedBy_id | crossref_primary_10_3389_fcell_2021_664099 crossref_primary_10_3390_agriculture13071426 crossref_primary_10_1111_cpr_13059 crossref_primary_10_1038_s41598_024_55464_0 crossref_primary_10_1016_j_anireprosci_2019_106125 crossref_primary_10_1016_j_anireprosci_2022_106987 crossref_primary_10_2478_aoas_2021_0046 crossref_primary_10_3390_ijms23094572 crossref_primary_10_3389_fcell_2025_1628953 crossref_primary_10_3390_ani11113062 crossref_primary_10_3390_ijms22115841 crossref_primary_10_1089_cell_2022_0003 crossref_primary_10_1016_j_theriogenology_2023_11_031 crossref_primary_10_2478_aoas_2021_0009 crossref_primary_10_1038_s41574_019_0280_9 crossref_primary_10_1016_j_theriogenology_2020_04_036 crossref_primary_10_1002_mrd_23627 crossref_primary_10_3390_ijms23041969 crossref_primary_10_1089_cell_2020_0022 crossref_primary_10_1111_rda_13942 crossref_primary_10_3390_ijms22105327 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1089/cell.2018.0050 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2152-4998 |
| ExternalDocumentID | 30735075 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- 0R~ 0VX 53G AAHBH ABBKN ACGFS ACIWK ACPRK ADBBV AENEX AFRAH ALMA_UNASSIGNED_HOLDINGS BNQNF CAG CGR COF CUY CVF DU5 EBS ECM EIF F5P IAO IGS IHR IM4 ITC NPM O9- RML UE5 7X8 SCNPE |
| ID | FETCH-LOGICAL-c419t-1a1fb53e88b8091b6f253f8ac30d7419fc5b8383957bba6fe0a449d6ddd2d1252 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 24 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000463363900006&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 2152-4998 |
| IngestDate | Fri Sep 05 06:58:39 EDT 2025 Thu Apr 03 06:49:53 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | somatic cell nuclear transfer embryo quality bovine cytoplasm injection cloning technology reprogramming efficiency |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c419t-1a1fb53e88b8091b6f253f8ac30d7419fc5b8383957bba6fe0a449d6ddd2d1252 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://pubmed.ncbi.nlm.nih.gov/PMC6383574 |
| PMID | 30735075 |
| PQID | 2185565877 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_2185565877 pubmed_primary_30735075 |
| PublicationCentury | 2000 |
| PublicationDate | 2019-02-00 20190201 |
| PublicationDateYYYYMMDD | 2019-02-01 |
| PublicationDate_xml | – month: 02 year: 2019 text: 2019-02-00 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Cellular reprogramming |
| PublicationTitleAlternate | Cell Reprogram |
| PublicationYear | 2019 |
| SSID | ssj0000331086 |
| Score | 2.3215709 |
| Snippet | Somatic cell nuclear transfer (SCNT) is a useful technology; however, its efficiency is low. In this study, we investigated the effects of cytoplasmic transfer... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 51 |
| SubjectTerms | Animals Blastocyst - cytology Cattle - embryology Cloning, Organism Cytoplasm DNA (Cytosine-5-)-Methyltransferases - genetics DNA (Cytosine-5-)-Methyltransferases - metabolism DNA Methylation Embryonic Development Female Mitochondria - metabolism Nuclear Transfer Techniques - veterinary Oocytes - cytology |
| Title | Improves the In Vitro Developmental Competence and Reprogramming Efficiency of Cloned Bovine Embryos by Additional Complimentary Cytoplasm |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/30735075 https://www.proquest.com/docview/2185565877 |
| Volume | 21 |
| WOSCitedRecordID | wos000463363900006&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpZ1LS8QwEMeDT_Di-_0ggtdi2yRNepJ1WVHQZQ-67G1pXrCw267bVehX8FM7abrqRRC89JYSksnML9Pp_BG6gnusltpGgYolDahgcOasYAHj3ETSAlPUTX36j7zbFYNB2msSbmVTVrnwibWj1oVyOfJrCEUM4ENwfjN9DZxqlPu62khoLKNVAijjrJoPxFeOJSTECQk5fTkIUwHAvVj0bRTptcuMu9ouV0zJwt8Js440d1v_neM22mwYE7e8UeygJZPvonWvOlntoQ-fSDAlBvjDDznuj-azAv8oH4LB7S-exlmuMXC6L-SaQKjDnbrvhPtpExcWt8cF-Gp863ITBncmclYVJZYVbmk98qnG-nVjLyQwq3C7mhdTwPbJPnq56zy374NGkiFQNErnQZRFVjJihJACSEMmNmbEikyRUAObpFYxKeDSmzIuZZZYE2aUpjrRWscaWCo-QCs5TOoIYaI4pTILWcwlBUsSyohEk0wmhqiU2mN0uVjkIZi8260sN8VbOfxe5mN06HdqOPW9OYbOZQHispM_jD5FG7D9qa_BPkOrFg68OUdr6n0-KmcXtS3Bs9t7-gReDtaU |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Improves+the+In+Vitro+Developmental+Competence+and+Reprogramming+Efficiency+of+Cloned+Bovine+Embryos+by+Additional+Complimentary+Cytoplasm&rft.jtitle=Cellular+reprogramming&rft.au=Xu%2C+Lianguang&rft.au=Mesalam%2C+Ayman&rft.au=Lee%2C+Kyeong-Lim&rft.au=Song%2C+Seok-Hwan&rft.date=2019-02-01&rft.issn=2152-4998&rft.eissn=2152-4998&rft.volume=21&rft.issue=1&rft.spage=51&rft_id=info:doi/10.1089%2Fcell.2018.0050&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2152-4998&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2152-4998&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2152-4998&client=summon |