Ruminococcus gnavus ameliorates atopic dermatitis by enhancing Treg cell and metabolites in BALB/c mice

Background Ruminococcus gnavus (R. gnavus) are mucin‐degrading gut bacteria that play a key role in the early colonization of the gut by serving as endogenous sources of nutrients. They can also influence immune development. We had previously reported a lower abundance of R. gnavus in infants with a...

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Vydáno v:Pediatric allergy and immunology Ročník 33; číslo 1; s. e13678 - n/a
Hlavní autoři: Ahn, Jae‐Rin, Lee, Seung‐Hwa, Kim, Byunghyun, Nam, Myung Hee, Ahn, Yoon Kyung, Park, Yoon Mee, Jeong, Seon‐Mi, Park, Min Jee, Song, Kun Baek, Lee, So‐Yeon, Hong, Soo‐Jong, Eigenmann, Philippe
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Wiley Subscription Services, Inc 01.01.2022
Témata:
Animals
Antibiotics
Atopic dermatitis
CD4 antigen
Cecum
Clostridiales
Dermatitis
Dermatitis, Atopic
Disease Models, Animal
Dysbacteriosis
Eczema
Fatty acids
Female
Foxp3 protein
Humans
Immunoglobulin E
Immunomodulation
Lymph nodes
Lymphocytes T
Mice
Mice, Inbred BALB C
mRNA
Mucin
Oral administration
Ovalbumin
Phenotypes
regulatory T cells
Ruminococcus
Ruminococcus gnavus
short‐chain fatty acids
Skin
T-Lymphocytes, Regulatory
regulatory T cells
atopic dermatitis
short-chain fatty acids
Ruminococcus gnavus
ISSN:0905-6157, 1399-3038, 1399-3038
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Shrnutí:Background Ruminococcus gnavus (R. gnavus) are mucin‐degrading gut bacteria that play a key role in the early colonization of the gut by serving as endogenous sources of nutrients. They can also influence immune development. We had previously reported a lower abundance of R. gnavus in infants with atopic dermatitis (AD) compared with that in healthy subjects. However, the underlying mechanisms remain unclear. In this study, we investigated the effect of orally administered R. gnavus on antibiotic treatment‐induced gut dysbiosis (and the underlying mechanism) in a mouse model of AD. Methods Four‐week‐old female BALB/C mice were administered antibiotic cocktails for 2 weeks. R. gnavus was orally administered throughout the study duration. At 6 weeks of age, AD was induced by epidermal sensitization with ovalbumin. AD phenotypes and systemic and gut immune responses were investigated. Results Orally administered R. gnavus significantly reduced AD‐associated parameters (i.e., transepidermal water loss, clinical score, total serum immunoglobulin (Ig) E level, OVA‐specific IgE level, and skin inflammation). R. gnavus treatment also resulted in significant downregulation of T helper 2–related cytokine mRNA and upregulation of interleukin (IL)‐10 and Foxp3 in the skin. The population of CD4+FOXP3+ T cells in mesenteric‐ and skin‐draining lymph nodes and butyrate levels in the cecum increased in R. gnavus‐administered AD mice. Conclusions Immune modulation by orally administered R. gnavus may alleviate AD symptoms through the enhancement of regulatory T‐cell counts and short‐chain fatty acids production in AD mice.
Bibliografie:Jae‐Rin Ahn and Seung‐Hwa Lee contributed equally to this work.
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ISSN:0905-6157
1399-3038
1399-3038
DOI:10.1111/pai.13678