Study of melanocyte density and epidermal thickness in vulvar lichen sclerosus lesions

Objective This study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS). Methods Vulvar skin tissues were collected from 15 VLS female patients in Beijing Hospital, classified into early (n = 7) and late VLS (n = 8) groups according to pathologica...

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Veröffentlicht in:International journal of dermatology Jg. 60; H. 8; S. 991 - 995
Hauptverfasser: Fu, Yu, Bao, Yingqiu, Li, Ziyuan, Gao, Xiaoman, Chang, Jianmin
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Blackwell Publishing Ltd 01.08.2021
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ISSN:0011-9059, 1365-4632, 1365-4632
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Abstract Objective This study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS). Methods Vulvar skin tissues were collected from 15 VLS female patients in Beijing Hospital, classified into early (n = 7) and late VLS (n = 8) groups according to pathological manifestations. Melanocyte density and full epidermal and cell‐layer (from the bottom of the stratum corneum to that of the basal layer) thickness were calculated using an image analysis software. The control group was normal vulvar skin tissues from 15 females after plastic surgery. Results The early VLS (0.170 ± 0.071 µm) and late VLS (0.110 ± 0.035 µm) groups had significantly lower densities of epidermal melanocytes than the control group (0.275 ± 0.036) (F = 36.426, P < 0.001). The cell‐layer thickness did not differ between the early VLS (154.603 ± 121.984 µm) and control (176.974 ± 80.296 µm) groups (P = 0.899) but significantly decreased in the late VLS group (83.455 ± 37.129 µm) compared to the control group (P = 0.003). Conclusions Melanocyte density decreased in early and late VLS. The full epidermal and cell‐layer thickness did not significantly change in early VLS, but the cell‐layer thickness decreased in late VLS.
AbstractList This study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS).OBJECTIVEThis study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS).Vulvar skin tissues were collected from 15 VLS female patients in Beijing Hospital, classified into early (n = 7) and late VLS (n = 8) groups according to pathological manifestations. Melanocyte density and full epidermal and cell-layer (from the bottom of the stratum corneum to that of the basal layer) thickness were calculated using an image analysis software. The control group was normal vulvar skin tissues from 15 females after plastic surgery.METHODSVulvar skin tissues were collected from 15 VLS female patients in Beijing Hospital, classified into early (n = 7) and late VLS (n = 8) groups according to pathological manifestations. Melanocyte density and full epidermal and cell-layer (from the bottom of the stratum corneum to that of the basal layer) thickness were calculated using an image analysis software. The control group was normal vulvar skin tissues from 15 females after plastic surgery.The early VLS (0.170 ± 0.071 µm) and late VLS (0.110 ± 0.035 µm) groups had significantly lower densities of epidermal melanocytes than the control group (0.275 ± 0.036) (F = 36.426, P < 0.001). The cell-layer thickness did not differ between the early VLS (154.603 ± 121.984 µm) and control (176.974 ± 80.296 µm) groups (P = 0.899) but significantly decreased in the late VLS group (83.455 ± 37.129 µm) compared to the control group (P = 0.003).RESULTSThe early VLS (0.170 ± 0.071 µm) and late VLS (0.110 ± 0.035 µm) groups had significantly lower densities of epidermal melanocytes than the control group (0.275 ± 0.036) (F = 36.426, P < 0.001). The cell-layer thickness did not differ between the early VLS (154.603 ± 121.984 µm) and control (176.974 ± 80.296 µm) groups (P = 0.899) but significantly decreased in the late VLS group (83.455 ± 37.129 µm) compared to the control group (P = 0.003).Melanocyte density decreased in early and late VLS. The full epidermal and cell-layer thickness did not significantly change in early VLS, but the cell-layer thickness decreased in late VLS.CONCLUSIONSMelanocyte density decreased in early and late VLS. The full epidermal and cell-layer thickness did not significantly change in early VLS, but the cell-layer thickness decreased in late VLS.
ObjectiveThis study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS).MethodsVulvar skin tissues were collected from 15 VLS female patients in Beijing Hospital, classified into early (n = 7) and late VLS (n = 8) groups according to pathological manifestations. Melanocyte density and full epidermal and cell‐layer (from the bottom of the stratum corneum to that of the basal layer) thickness were calculated using an image analysis software. The control group was normal vulvar skin tissues from 15 females after plastic surgery.ResultsThe early VLS (0.170 ± 0.071 µm) and late VLS (0.110 ± 0.035 µm) groups had significantly lower densities of epidermal melanocytes than the control group (0.275 ± 0.036) (F = 36.426, P < 0.001). The cell‐layer thickness did not differ between the early VLS (154.603 ± 121.984 µm) and control (176.974 ± 80.296 µm) groups (P = 0.899) but significantly decreased in the late VLS group (83.455 ± 37.129 µm) compared to the control group (P = 0.003).ConclusionsMelanocyte density decreased in early and late VLS. The full epidermal and cell‐layer thickness did not significantly change in early VLS, but the cell‐layer thickness decreased in late VLS.
Objective This study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS). Methods Vulvar skin tissues were collected from 15 VLS female patients in Beijing Hospital, classified into early (n = 7) and late VLS (n = 8) groups according to pathological manifestations. Melanocyte density and full epidermal and cell‐layer (from the bottom of the stratum corneum to that of the basal layer) thickness were calculated using an image analysis software. The control group was normal vulvar skin tissues from 15 females after plastic surgery. Results The early VLS (0.170 ± 0.071 µm) and late VLS (0.110 ± 0.035 µm) groups had significantly lower densities of epidermal melanocytes than the control group (0.275 ± 0.036) (F = 36.426, P < 0.001). The cell‐layer thickness did not differ between the early VLS (154.603 ± 121.984 µm) and control (176.974 ± 80.296 µm) groups (P = 0.899) but significantly decreased in the late VLS group (83.455 ± 37.129 µm) compared to the control group (P = 0.003). Conclusions Melanocyte density decreased in early and late VLS. The full epidermal and cell‐layer thickness did not significantly change in early VLS, but the cell‐layer thickness decreased in late VLS.
This study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS). Vulvar skin tissues were collected from 15 VLS female patients in Beijing Hospital, classified into early (n = 7) and late VLS (n = 8) groups according to pathological manifestations. Melanocyte density and full epidermal and cell-layer (from the bottom of the stratum corneum to that of the basal layer) thickness were calculated using an image analysis software. The control group was normal vulvar skin tissues from 15 females after plastic surgery. The early VLS (0.170 ± 0.071 µm) and late VLS (0.110 ± 0.035 µm) groups had significantly lower densities of epidermal melanocytes than the control group (0.275 ± 0.036) (F = 36.426, P < 0.001). The cell-layer thickness did not differ between the early VLS (154.603 ± 121.984 µm) and control (176.974 ± 80.296 µm) groups (P = 0.899) but significantly decreased in the late VLS group (83.455 ± 37.129 µm) compared to the control group (P = 0.003). Melanocyte density decreased in early and late VLS. The full epidermal and cell-layer thickness did not significantly change in early VLS, but the cell-layer thickness decreased in late VLS.
Author Chang, Jianmin
Bao, Yingqiu
Gao, Xiaoman
Fu, Yu
Li, Ziyuan
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Cites_doi 10.1080/00015550310015419
10.1097/00000372-200204000-00001
10.1111/exd.13858
10.1007/s40257-012-0006-4
10.1111/j.1365-2230.2011.04088.x
10.4103/ijstd.IJSTD_23_19
10.1111/1346-8138.14533
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Funding source: CAMS Innovation fund for Medical Science: 2018‐12M‐1‐002
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Snippet Objective This study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS). Methods Vulvar skin tissues were...
This study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS). Vulvar skin tissues were collected from 15...
ObjectiveThis study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS).MethodsVulvar skin tissues were...
This study aimed to analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus (VLS).OBJECTIVEThis study aimed to analyze changes...
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SubjectTerms Density
Image analysis
Image processing
Lesions
Melanocytes
Plastic surgery
Skin
Stratum corneum
Surgery
Thickness
Title Study of melanocyte density and epidermal thickness in vulvar lichen sclerosus lesions
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