Radiation-induced changes in DNA methylation and their relationship to chromosome aberrations in nuclear power plant workers

Abstract Purpose: We investigated the association between occupational radiation exposure and DNA methylation changes in nuclear power plant workers. We also evaluated whether radiation- induced DNA methylation alterations are associated with chromosome aberrations. Materials and methods: The study...

Full description

Saved in:
Bibliographic Details
Published in:International journal of radiation biology Vol. 91; no. 2; pp. 142 - 149
Main Authors: Lee, Younghyun, Kim, Yang Jee, Choi, Young Joo, Lee, Joong Won, Lee, Sunyeong, Cho, Yoon Hee, Chung, Hai Won
Format: Journal Article
Language:English
Published: England Informa Healthcare 01.02.2015
Taylor & Francis
Subjects:
Adult
Aged
Case-Control Studies
chromosome aberrations
Chromosome Aberrations - radiation effects
DNA methylation
DNA Methylation - radiation effects
Humans
Male
Middle Aged
nuclear power plant workers
Nuclear Power Plants
Occupational Exposure - adverse effects
Radiation
Repetitive Sequences, Nucleic Acid - genetics
nuclear power plant workers
DNA methylation
chromosome aberrations
Radiation
ISSN:0955-3002, 1362-3095, 1362-3095
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Purpose: We investigated the association between occupational radiation exposure and DNA methylation changes in nuclear power plant workers. We also evaluated whether radiation- induced DNA methylation alterations are associated with chromosome aberrations. Materials and methods: The study population included 170 radiation-exposed workers and 30 controls. We measured global, long interspersed nuclear element-1 (LINE-1), and satellite 2 methylation levels in blood leukocyte DNA. The analysis of chromosome aberrations was performed on peripheral lymphocytes. Results: Global DNA methylation levels were lower in radiation-exposed workers than in controls. The methylation levels were negatively associated with the recent 1.5-year radiation dose in a multiple linear regression model (β = − 0.0088, p ≤ 0.001); the levels increased proportionally with the total cumulative dose in radiation-exposed workers. LINE-1 methylation levels were higher in radiation-exposed workers than in controls and were significantly associated with the total cumulative radiation dose in a multiple linear regression model (β = − 0.031, p = 0.035). Global DNA methylation levels were also correlated with chromosome aberrations among workers. Workers with low global methylation levels had a higher frequency of chromosome aberrations than did subjects with high global methylation levels. Conclusion: Occupational exposure to low-dose radiation could affect DNA methylation levels, and the radiation-induced DNA methylation alterations may be associated with chromosome aberrations.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0955-3002
1362-3095
1362-3095
DOI:10.3109/09553002.2015.969847