CcpA is important for growth and virulence of Enterococcus faecium
The collagen adhesin Acm was the first virulence determinant reported to be important for the pathogenesis of Enterococcus faecium in a rat infective endocarditis model. We had previously reported that there was a slight growth delay associated with acm allelic replacement (cat) mutant strain TX6051...
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| Vydáno v: | Infection and immunity Ročník 82; číslo 9; s. 3580 |
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01.09.2014
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| Abstract | The collagen adhesin Acm was the first virulence determinant reported to be important for the pathogenesis of Enterococcus faecium in a rat infective endocarditis model. We had previously reported that there was a slight growth delay associated with acm allelic replacement (cat) mutant strain TX6051 used in that study. Recently, we generated a nonpolar markerless acm deletion mutant and did not observe a delay in growth. We therefore performed comparative genome sequence analysis of wild-type strain TX82 and TX6051 and found a single mutation, a nonsense mutation in the ccpA gene of TX6051. After correcting this mutation, the growth defect of TX6051 was abolished, implicating a role for CcpA in the growth of E. faecium. To confirm this, we created a ccpA deletion mutant of TX82, which also exhibited a slight delay in growth. Furthermore, the ccpA deletion mutant was attenuated (P = 0.0024) in a mixed-inoculum (TX82 plus TX82 ΔccpA) rat endocarditis model and also in an in vitro competitive growth assay; a ccpA-complemented strain showed neither reduced growth nor reduced virulence. We also found attenuation in the endocarditis model with the new acm deletion mutant although not as great as that previously observed with TX6051 carrying the ccpA mutation. Taken together, our data confirm the role of Acm in the pathogenesis of endocarditis. We also show that CcpA affects the growth of E. faecium, that an intact ccpA gene is important for full virulence, and that a ccpA mutation was partly responsible for the highly attenuated phenotype of TX6051. |
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| AbstractList | The collagen adhesin Acm was the first virulence determinant reported to be important for the pathogenesis of Enterococcus faecium in a rat infective endocarditis model. We had previously reported that there was a slight growth delay associated with acm allelic replacement (cat) mutant strain TX6051 used in that study. Recently, we generated a nonpolar markerless acm deletion mutant and did not observe a delay in growth. We therefore performed comparative genome sequence analysis of wild-type strain TX82 and TX6051 and found a single mutation, a nonsense mutation in the ccpA gene of TX6051. After correcting this mutation, the growth defect of TX6051 was abolished, implicating a role for CcpA in the growth of E. faecium. To confirm this, we created a ccpA deletion mutant of TX82, which also exhibited a slight delay in growth. Furthermore, the ccpA deletion mutant was attenuated (P = 0.0024) in a mixed-inoculum (TX82 plus TX82 ΔccpA) rat endocarditis model and also in an in vitro competitive growth assay; a ccpA-complemented strain showed neither reduced growth nor reduced virulence. We also found attenuation in the endocarditis model with the new acm deletion mutant although not as great as that previously observed with TX6051 carrying the ccpA mutation. Taken together, our data confirm the role of Acm in the pathogenesis of endocarditis. We also show that CcpA affects the growth of E. faecium, that an intact ccpA gene is important for full virulence, and that a ccpA mutation was partly responsible for the highly attenuated phenotype of TX6051. The collagen adhesin Acm was the first virulence determinant reported to be important for the pathogenesis of Enterococcus faecium in a rat infective endocarditis model. We had previously reported that there was a slight growth delay associated with acm allelic replacement (cat) mutant strain TX6051 used in that study. Recently, we generated a nonpolar markerless acm deletion mutant and did not observe a delay in growth. We therefore performed comparative genome sequence analysis of wild-type strain TX82 and TX6051 and found a single mutation, a nonsense mutation in the ccpA gene of TX6051. After correcting this mutation, the growth defect of TX6051 was abolished, implicating a role for CcpA in the growth of E. faecium. To confirm this, we created a ccpA deletion mutant of TX82, which also exhibited a slight delay in growth. Furthermore, the ccpA deletion mutant was attenuated (P = 0.0024) in a mixed-inoculum (TX82 plus TX82 ΔccpA) rat endocarditis model and also in an in vitro competitive growth assay; a ccpA-complemented strain showed neither reduced growth nor reduced virulence. We also found attenuation in the endocarditis model with the new acm deletion mutant although not as great as that previously observed with TX6051 carrying the ccpA mutation. Taken together, our data confirm the role of Acm in the pathogenesis of endocarditis. We also show that CcpA affects the growth of E. faecium, that an intact ccpA gene is important for full virulence, and that a ccpA mutation was partly responsible for the highly attenuated phenotype of TX6051.The collagen adhesin Acm was the first virulence determinant reported to be important for the pathogenesis of Enterococcus faecium in a rat infective endocarditis model. We had previously reported that there was a slight growth delay associated with acm allelic replacement (cat) mutant strain TX6051 used in that study. Recently, we generated a nonpolar markerless acm deletion mutant and did not observe a delay in growth. We therefore performed comparative genome sequence analysis of wild-type strain TX82 and TX6051 and found a single mutation, a nonsense mutation in the ccpA gene of TX6051. After correcting this mutation, the growth defect of TX6051 was abolished, implicating a role for CcpA in the growth of E. faecium. To confirm this, we created a ccpA deletion mutant of TX82, which also exhibited a slight delay in growth. Furthermore, the ccpA deletion mutant was attenuated (P = 0.0024) in a mixed-inoculum (TX82 plus TX82 ΔccpA) rat endocarditis model and also in an in vitro competitive growth assay; a ccpA-complemented strain showed neither reduced growth nor reduced virulence. We also found attenuation in the endocarditis model with the new acm deletion mutant although not as great as that previously observed with TX6051 carrying the ccpA mutation. Taken together, our data confirm the role of Acm in the pathogenesis of endocarditis. We also show that CcpA affects the growth of E. faecium, that an intact ccpA gene is important for full virulence, and that a ccpA mutation was partly responsible for the highly attenuated phenotype of TX6051. |
| Author | Murray, Barbara E Singh, Kavindra V Weinstock, George M Somarajan, Sudha R Roh, Jung H |
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| References | 21299645 - Mol Microbiol. 2011 Feb;79(4):882-99 21266081 - BMC Microbiol. 2011;11(1):20 15292123 - J Bacteriol. 2004 Aug;186(16):5221-9 18947320 - Infect Control Hosp Epidemiol. 2008 Nov;29(11):996-1011 18591238 - Infect Immun. 2008 Sep;76(9):4110-9 16996131 - Plasmid. 2007 Mar;57(2):131-44 18832325 - Microbiology. 2008 Oct;154(Pt 10):3199-211 18223086 - J Bacteriol. 2008 Apr;190(7):2340-9 12552437 - J Infect Dis. 2003 Feb 1;187(3):508-12 9753005 - FEMS Immunol Med Microbiol. 1998 Aug;21(4):323-31 21109532 - Nucleic Acids Res. 2011 Jan;39(Database issue):D225-9 23447698 - J Infect Dis. 2013 Jun 1;207(11):1780-6 23974022 - J Bacteriol. 2013 Oct;195(20):4761-8 23963180 - MBio. 2013;4(4). pii: e00534-13. doi: 10.1128/mBio.00534-13 23274986 - J Microbiol. 2012 Dec;50(6):994-1002 24606170 - FEMS Microbiol Lett. 2014 Apr;353(2):151-6 7699051 - J Clin Microbiol. 1995 Jan;33(1):24-7 17257059 - PLoS Pathog. 2007 Jan;3(1):e7 18347047 - Infect Immun. 2008 May;76(5):2044-50 12622825 - Mol Microbiol. 2003 Mar;47(6):1733-47 21899450 - N Engl J Med. 2011 Sep 8;365(10):892-900 22421879 - Nat Rev Microbiol. 2012 Apr;10(4):266-78 20072611 - PLoS Pathog. 2010 Jan;6(1):e1000716 11004180 - J Bacteriol. 2000 Oct;182(20):5799-806 22989714 - Nucleic Acids Res. 2012 Nov;40(21):10701-18 14665673 - Microbiol Mol Biol Rev. 2003 Dec;67(4):475-90 22856458 - J Proteome Res. 2012 Sep 7;11(9):4654-61 18591236 - Infect Immun. 2008 Sep;76(9):4120-8 20971911 - J Bacteriol. 2011 Jan;193(1):52-62 15155680 - Infect Immun. 2004 Jun;72(6):3658-63 10706902 - N Engl J Med. 2000 Mar 9;342(10):710-21 19821720 - J Infect Dis. 2009 Nov 15;200(10):1566-73 21964732 - Microbiology. 2011 Dec;157(Pt 12):3458-68 12618459 - J Bacteriol. 2003 Mar;185(6):1951-7 18230719 - Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1698-703 16321938 - J Bacteriol. 2005 Dec;187(24):8340-9 23209408 - PLoS Pathog. 2012;8(11):e1003033 19193843 - J Clin Microbiol. 2009 Apr;47(4):896-901 16569828 - Antimicrob Agents Chemother. 2006 Apr;50(4):1183-94 16391062 - Appl Environ Microbiol. 2006 Jan;72(1):334-45 18765726 - Infect Immun. 2008 Nov;76(11):4944-51 23882129 - Genome Biol Evol. 2013;5(8):1524-35 9003306 - Mol Gen Genet. 1996 Nov 27;253(1-2):217-24 11265956 - Lancet. 2001 Mar 17;357(9259):853-5 23447697 - J Infect Dis. 2013 Jun 1;207(11):1633-6 |
| References_xml | – reference: 19821720 - J Infect Dis. 2009 Nov 15;200(10):1566-73 – reference: 21964732 - Microbiology. 2011 Dec;157(Pt 12):3458-68 – reference: 15155680 - Infect Immun. 2004 Jun;72(6):3658-63 – reference: 21266081 - BMC Microbiol. 2011;11(1):20 – reference: 7699051 - J Clin Microbiol. 1995 Jan;33(1):24-7 – reference: 12622825 - Mol Microbiol. 2003 Mar;47(6):1733-47 – reference: 17257059 - PLoS Pathog. 2007 Jan;3(1):e7 – reference: 21899450 - N Engl J Med. 2011 Sep 8;365(10):892-900 – reference: 14665673 - Microbiol Mol Biol Rev. 2003 Dec;67(4):475-90 – reference: 23447698 - J Infect Dis. 2013 Jun 1;207(11):1780-6 – reference: 18832325 - Microbiology. 2008 Oct;154(Pt 10):3199-211 – reference: 22989714 - Nucleic Acids Res. 2012 Nov;40(21):10701-18 – reference: 10706902 - N Engl J Med. 2000 Mar 9;342(10):710-21 – reference: 16321938 - J Bacteriol. 2005 Dec;187(24):8340-9 – reference: 18765726 - Infect Immun. 2008 Nov;76(11):4944-51 – reference: 24606170 - FEMS Microbiol Lett. 2014 Apr;353(2):151-6 – reference: 9753005 - FEMS Immunol Med Microbiol. 1998 Aug;21(4):323-31 – reference: 18230719 - Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1698-703 – reference: 23209408 - PLoS Pathog. 2012;8(11):e1003033 – reference: 16569828 - Antimicrob Agents Chemother. 2006 Apr;50(4):1183-94 – reference: 23882129 - Genome Biol Evol. 2013;5(8):1524-35 – reference: 22856458 - J Proteome Res. 2012 Sep 7;11(9):4654-61 – reference: 23274986 - J Microbiol. 2012 Dec;50(6):994-1002 – reference: 18591238 - Infect Immun. 2008 Sep;76(9):4110-9 – reference: 18591236 - Infect Immun. 2008 Sep;76(9):4120-8 – reference: 23963180 - MBio. 2013;4(4). pii: e00534-13. doi: 10.1128/mBio.00534-13 – reference: 11004180 - J Bacteriol. 2000 Oct;182(20):5799-806 – reference: 16391062 - Appl Environ Microbiol. 2006 Jan;72(1):334-45 – reference: 23974022 - J Bacteriol. 2013 Oct;195(20):4761-8 – reference: 22421879 - Nat Rev Microbiol. 2012 Apr;10(4):266-78 – reference: 12618459 - J Bacteriol. 2003 Mar;185(6):1951-7 – reference: 18347047 - Infect Immun. 2008 May;76(5):2044-50 – reference: 21299645 - Mol Microbiol. 2011 Feb;79(4):882-99 – reference: 9003306 - Mol Gen Genet. 1996 Nov 27;253(1-2):217-24 – reference: 19193843 - J Clin Microbiol. 2009 Apr;47(4):896-901 – reference: 12552437 - J Infect Dis. 2003 Feb 1;187(3):508-12 – reference: 20072611 - PLoS Pathog. 2010 Jan;6(1):e1000716 – reference: 18223086 - J Bacteriol. 2008 Apr;190(7):2340-9 – reference: 15292123 - J Bacteriol. 2004 Aug;186(16):5221-9 – reference: 20971911 - J Bacteriol. 2011 Jan;193(1):52-62 – reference: 23447697 - J Infect Dis. 2013 Jun 1;207(11):1633-6 – reference: 21109532 - Nucleic Acids Res. 2011 Jan;39(Database issue):D225-9 – reference: 16996131 - Plasmid. 2007 Mar;57(2):131-44 – reference: 11265956 - Lancet. 2001 Mar 17;357(9259):853-5 – reference: 18947320 - Infect Control Hosp Epidemiol. 2008 Nov;29(11):996-1011 |
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| SubjectTerms | Adhesins, Bacterial - genetics Adhesins, Bacterial - metabolism Amino Acid Sequence Animals Bacterial Adhesion - genetics Bacterial Proteins - genetics Bacterial Proteins - metabolism Biofilms - growth & development Endocarditis, Bacterial Enterococcus faecium - genetics Enterococcus faecium - metabolism Molecular Sequence Data Rats Sequence Deletion - genetics Virulence - genetics |
| Title | CcpA is important for growth and virulence of Enterococcus faecium |
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