Gene therapy for diabetes mellitus in rats by intramuscular injection of lentivirus containing insulin gene

We assessed therapeutic potential of intramuscular insulin gene delivery in a diabetic murine model. The human proinsulin gene cDNA engineered with concensus furin cleavage sequences was inserted into an advanced lentiviral vector that contained CMV early promoter. After injection of concentrated le...

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Bibliographic Details
Published in:Diabetes research and clinical practice Vol. 71; no. 3; pp. 233 - 240
Main Authors: Oh, Tae Keun, Li, Mei Zi, Kim, Seung Taik
Format: Journal Article
Language:English
Published: Ireland Elsevier Ireland Ltd 01.03.2006
Subjects:
Animals
Cell Line
Diabetes Mellitus, Experimental - drug therapy
Genes, Reporter
Genetic Therapy - methods
Genetic Vectors
Green Fluorescent Proteins - genetics
HeLa Cells
Humans
Injections, Intramuscular
Insulin - administration & dosage
Insulin - genetics
Insulin - therapeutic use
Insulin gene therapy
Intramuscular injection
Lentivirus
Lentivirus - genetics
Male
Mice
Proinsulin - genetics
Promoter Regions, Genetic
Rats
Rats, Inbred F344
Insulin gene therapy
Lentivirus
Intramuscular injection
ISSN:0168-8227, 1872-8227
Online Access:Get full text
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Summary:We assessed therapeutic potential of intramuscular insulin gene delivery in a diabetic murine model. The human proinsulin gene cDNA engineered with concensus furin cleavage sequences was inserted into an advanced lentiviral vector that contained CMV early promoter. After injection of concentrated lentiviral vector (3.5 μg p24 Gag antigen) carrying the insulin gene into the thigh muscle, treated rats demonstrated an increase in body weight, increased survivability, attenuated the hyperglycemic response as well as prevented the formation of ketoacidosis. For these reasons, the intraparenchymal injection of lentiviral vectors into the skeletal muscle to ectopically produce insulin may be an easy and therapeutic treatment modality for type 1 diabetes mellitus.
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ISSN:0168-8227
1872-8227
DOI:10.1016/j.diabres.2005.08.005