Aerobactin, but not yersiniabactin, salmochelin, or enterobactin, enables the growth/survival of hypervirulent (hypermucoviscous) Klebsiella pneumoniae ex vivo and in vivo

The siderophore aerobactin is the dominant siderophore produced by hypervirulent Klebsiella pneumoniae (hvKP) and was previously shown to be a major virulence factor in systemic infection. However, strains of hvKP commonly produce the additional siderophores yersiniabactin, salmochelin, and enteroba...

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Published in:	Infection and immunity Vol. 83; no. 8; p. 3325
Main Authors:	Russo, Thomas A, Olson, Ruth, MacDonald, Ulrike, Beanan, Janet, Davidson, Bruce A
Format:	Journal Article
Language:	English
Published:	United States 01.08.2015
Subjects:	Animals Enterobactin - analogs & derivatives Enterobactin - metabolism Glucosides - metabolism Humans Hydroxamic Acids - metabolism Klebsiella Infections - microbiology Klebsiella pneumoniae - genetics Klebsiella pneumoniae - growth & development Klebsiella pneumoniae - metabolism Klebsiella pneumoniae - pathogenicity Male Mice Microbial Viability - drug effects Phenols - metabolism Siderophores - metabolism Thiazoles - metabolism Virulence Young Adult
ISSN:	1098-5522, 1098-5522
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Abstract	The siderophore aerobactin is the dominant siderophore produced by hypervirulent Klebsiella pneumoniae (hvKP) and was previously shown to be a major virulence factor in systemic infection. However, strains of hvKP commonly produce the additional siderophores yersiniabactin, salmochelin, and enterobactin. The roles of these siderophores in hvKP infection have not been optimally defined. To that end, site-specific gene disruptions were created in hvKP1 (wild type), resulting in the generation of hvKP1ΔiucA (aerobactin deficient), hvKP1ΔiroB (salmochelin deficient), hvKP1ΔentB (enterobactin and salmochelin deficient), hvKP1Δirp2 (yersiniabactin deficient), and hvKP1ΔentBΔirp2 (enterobactin, salmochelin, and yersiniabactin deficient). The growth/survival of these constructs was compared to that of their wild-type parent hvKP1 ex vivo in human ascites fluid, human serum, and human urine and in vivo in mouse systemic infection and pulmonary challenge models. Interestingly, in contrast to aerobactin, the inability to produce enterobactin, salmochelin, or yersiniabactin individually or in combination did not decrease the ex vivo growth/survival in human ascites or serum or decrease virulence in the in vivo infection models. Surprisingly, none of the siderophores increased growth in human urine. In human ascites fluid supplemented with exogenous siderophores, siderophores increased the growth of hvKP1ΔiucA, with the relative activity being enterobactin > aerobactin > yersiniabactin > salmochelin, suggesting that the contribution of aerobactin to virulence is dependent on both innate biologic activity and quantity produced. Taken together, these data confirm and extend a role for aerobactin as a critical virulence factor for hvKP. Since it appears that aerobactin production is a defining trait of hvKP strains, this factor is a potential antivirulence target.
AbstractList	The siderophore aerobactin is the dominant siderophore produced by hypervirulent Klebsiella pneumoniae (hvKP) and was previously shown to be a major virulence factor in systemic infection. However, strains of hvKP commonly produce the additional siderophores yersiniabactin, salmochelin, and enterobactin. The roles of these siderophores in hvKP infection have not been optimally defined. To that end, site-specific gene disruptions were created in hvKP1 (wild type), resulting in the generation of hvKP1ΔiucA (aerobactin deficient), hvKP1ΔiroB (salmochelin deficient), hvKP1ΔentB (enterobactin and salmochelin deficient), hvKP1Δirp2 (yersiniabactin deficient), and hvKP1ΔentBΔirp2 (enterobactin, salmochelin, and yersiniabactin deficient). The growth/survival of these constructs was compared to that of their wild-type parent hvKP1 ex vivo in human ascites fluid, human serum, and human urine and in vivo in mouse systemic infection and pulmonary challenge models. Interestingly, in contrast to aerobactin, the inability to produce enterobactin, salmochelin, or yersiniabactin individually or in combination did not decrease the ex vivo growth/survival in human ascites or serum or decrease virulence in the in vivo infection models. Surprisingly, none of the siderophores increased growth in human urine. In human ascites fluid supplemented with exogenous siderophores, siderophores increased the growth of hvKP1ΔiucA, with the relative activity being enterobactin > aerobactin > yersiniabactin > salmochelin, suggesting that the contribution of aerobactin to virulence is dependent on both innate biologic activity and quantity produced. Taken together, these data confirm and extend a role for aerobactin as a critical virulence factor for hvKP. Since it appears that aerobactin production is a defining trait of hvKP strains, this factor is a potential antivirulence target. The siderophore aerobactin is the dominant siderophore produced by hypervirulent Klebsiella pneumoniae (hvKP) and was previously shown to be a major virulence factor in systemic infection. However, strains of hvKP commonly produce the additional siderophores yersiniabactin, salmochelin, and enterobactin. The roles of these siderophores in hvKP infection have not been optimally defined. To that end, site-specific gene disruptions were created in hvKP1 (wild type), resulting in the generation of hvKP1ΔiucA (aerobactin deficient), hvKP1ΔiroB (salmochelin deficient), hvKP1ΔentB (enterobactin and salmochelin deficient), hvKP1Δirp2 (yersiniabactin deficient), and hvKP1ΔentBΔirp2 (enterobactin, salmochelin, and yersiniabactin deficient). The growth/survival of these constructs was compared to that of their wild-type parent hvKP1 ex vivo in human ascites fluid, human serum, and human urine and in vivo in mouse systemic infection and pulmonary challenge models. Interestingly, in contrast to aerobactin, the inability to produce enterobactin, salmochelin, or yersiniabactin individually or in combination did not decrease the ex vivo growth/survival in human ascites or serum or decrease virulence in the in vivo infection models. Surprisingly, none of the siderophores increased growth in human urine. In human ascites fluid supplemented with exogenous siderophores, siderophores increased the growth of hvKP1ΔiucA, with the relative activity being enterobactin > aerobactin > yersiniabactin > salmochelin, suggesting that the contribution of aerobactin to virulence is dependent on both innate biologic activity and quantity produced. Taken together, these data confirm and extend a role for aerobactin as a critical virulence factor for hvKP. Since it appears that aerobactin production is a defining trait of hvKP strains, this factor is a potential antivirulence target.The siderophore aerobactin is the dominant siderophore produced by hypervirulent Klebsiella pneumoniae (hvKP) and was previously shown to be a major virulence factor in systemic infection. However, strains of hvKP commonly produce the additional siderophores yersiniabactin, salmochelin, and enterobactin. The roles of these siderophores in hvKP infection have not been optimally defined. To that end, site-specific gene disruptions were created in hvKP1 (wild type), resulting in the generation of hvKP1ΔiucA (aerobactin deficient), hvKP1ΔiroB (salmochelin deficient), hvKP1ΔentB (enterobactin and salmochelin deficient), hvKP1Δirp2 (yersiniabactin deficient), and hvKP1ΔentBΔirp2 (enterobactin, salmochelin, and yersiniabactin deficient). The growth/survival of these constructs was compared to that of their wild-type parent hvKP1 ex vivo in human ascites fluid, human serum, and human urine and in vivo in mouse systemic infection and pulmonary challenge models. Interestingly, in contrast to aerobactin, the inability to produce enterobactin, salmochelin, or yersiniabactin individually or in combination did not decrease the ex vivo growth/survival in human ascites or serum or decrease virulence in the in vivo infection models. Surprisingly, none of the siderophores increased growth in human urine. In human ascites fluid supplemented with exogenous siderophores, siderophores increased the growth of hvKP1ΔiucA, with the relative activity being enterobactin > aerobactin > yersiniabactin > salmochelin, suggesting that the contribution of aerobactin to virulence is dependent on both innate biologic activity and quantity produced. Taken together, these data confirm and extend a role for aerobactin as a critical virulence factor for hvKP. Since it appears that aerobactin production is a defining trait of hvKP strains, this factor is a potential antivirulence target.
Author	Davidson, Bruce A Russo, Thomas A MacDonald, Ulrike Olson, Ruth Beanan, Janet
Author_xml	– sequence: 1 givenname: Thomas A surname: Russo fullname: Russo, Thomas A email: trusso@acsu.buffalo.edu organization: Veterans Administration Western New York Healthcare System, Buffalo, New York, USA Department of Medicine, University at Buffalo-State University of New York, Buffalo, New York, USA Department of Microbiology and Immunology, University at Buffalo-State University of New York, Buffalo, New York, USA The Witebsky Center for Microbial Pathogenesis, University at Buffalo-State University of New York, Buffalo, New York, USA trusso@acsu.buffalo.edu – sequence: 2 givenname: Ruth surname: Olson fullname: Olson, Ruth organization: Veterans Administration Western New York Healthcare System, Buffalo, New York, USA Department of Medicine, University at Buffalo-State University of New York, Buffalo, New York, USA – sequence: 3 givenname: Ulrike surname: MacDonald fullname: MacDonald, Ulrike organization: Veterans Administration Western New York Healthcare System, Buffalo, New York, USA Department of Medicine, University at Buffalo-State University of New York, Buffalo, New York, USA – sequence: 4 givenname: Janet surname: Beanan fullname: Beanan, Janet organization: Veterans Administration Western New York Healthcare System, Buffalo, New York, USA Department of Medicine, University at Buffalo-State University of New York, Buffalo, New York, USA – sequence: 5 givenname: Bruce A surname: Davidson fullname: Davidson, Bruce A organization: Veterans Administration Western New York Healthcare System, Buffalo, New York, USA Department of Anesthesiology, University at Buffalo-State University of New York, Buffalo, New York, USA Department of Pathology and Anatomical Sciences, University at Buffalo-State University of New York, Buffalo, New York, USA The Witebsky Center for Microbial Pathogenesis, University at Buffalo-State University of New York, Buffalo, New York, USA
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/26056379$$D View this record in MEDLINE/PubMed
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PublicationTitleAlternate	Infect Immun
PublicationYear	2015
References	24678611 - BMC Infect Dis. 2014;14:176 19342674 - J Immunol. 2009 Apr 15;182(8):4947-56 10376834 - Microbiology. 1999 May;145 ( Pt 5):1181-90 21158655 - N Engl J Med. 2010 Dec 16;363(25):2377-9 19319196 - PLoS One. 2009;4(3):e4982 23302790 - Virulence. 2013 Feb 15;4(2):107-18 18433330 - J Infect Dis. 2008 Jun 15;197(12):1717-27 17220312 - Infect Immun. 2007 Mar;75(3):1463-72 15276215 - Gene. 2004 Aug 4;337:189-98 20887813 - Nanomedicine. 2011 Feb;7(1):88-96 15531878 - Nature. 2004 Dec 16;432(7019):917-21 21576334 - Infect Immun. 2011 Aug;79(8):3309-16 19143563 - J Infect Dis. 2009 Feb 15;199(4):513-21 20382770 - J Bacteriol. 2010 Jun;192(12):3144-58 24236613 - Microb Drug Resist. 2014 Apr;20(2):150-5 24664504 - Infect Immun. 2014 Jun;82(6):2356-67 9636876 - Clin Infect Dis. 1998 Jun;26(6):1434-8 1872659 - Arch Intern Med. 1991 Aug;151(8):1557-9 6234017 - Biochemistry. 1984 May 8;23(10):2122-7 10231495 - Mol Microbiol. 1999 Apr;32(2):403-14 20376388 - Nat Prod Rep. 2010 May;27(5):637-57 16704562 - J Intern Med. 2006 Jun;259(6):606-14 17804665 - Microbiol Mol Biol Rev. 2007 Sep;71(3):413-51 379572 - Microbiol Rev. 1978 Mar;42(1):45-66 19447910 - J Bacteriol. 2009 Jul;191(14):4492-501 3532983 - Arch Intern Med. 1986 Oct;146(10):1913-6 23466435 - MMWR Morb Mortal Wkly Rep. 2013 Mar 8;62(9):165-70 2643575 - Infect Immun. 1989 Feb;57(2):546-52 21964731 - Microbiology. 2011 Dec;157(Pt 12):3446-57 6295467 - Biochemistry. 1982 Dec 7;21(25):6503-8 24476533 - J Proteome Res. 2014 Mar 7;13(3):1397-404 6455965 - Annu Rev Biochem. 1981;50:715-31 8335346 - Infect Immun. 1993 Aug;61(8):3164-74 22914622 - Sci Transl Med. 2012 Aug 22;4(148):148ra116 19433545 - Infect Immun. 2009 Jul;77(7):2657-71 21220482 - Infect Immun. 2011 Mar;79(3):1225-35 11897067 - Emerg Infect Dis. 2002 Feb;8(2):160-6 21918907 - Eur J Clin Microbiol Infect Dis. 2012 Jun;31(6):981-9 22039542 - PLoS One. 2011;6(10):e26734 23169997 - MBio. 2012;3(6). pii: e00224-11. doi: 10.1128/mBio.00224-11 15501752 - Infect Immun. 2004 Nov;72(11):6262-70 23516199 - Genome Announc. 2013 Mar 14;1(2):e0006513 23592085 - JAMA. 2013 Apr 17;309(15):1573-4 18486404 - Diagn Microbiol Infect Dis. 2008 Sep;62(1):1-6 24099919 - Clin Infect Dis. 2014 Jan;58(2):225-32
References_xml	– reference: 24664504 - Infect Immun. 2014 Jun;82(6):2356-67 – reference: 20382770 - J Bacteriol. 2010 Jun;192(12):3144-58 – reference: 23592085 - JAMA. 2013 Apr 17;309(15):1573-4 – reference: 6234017 - Biochemistry. 1984 May 8;23(10):2122-7 – reference: 24678611 - BMC Infect Dis. 2014;14:176 – reference: 18433330 - J Infect Dis. 2008 Jun 15;197(12):1717-27 – reference: 18486404 - Diagn Microbiol Infect Dis. 2008 Sep;62(1):1-6 – reference: 23466435 - MMWR Morb Mortal Wkly Rep. 2013 Mar 8;62(9):165-70 – reference: 21220482 - Infect Immun. 2011 Mar;79(3):1225-35 – reference: 10231495 - Mol Microbiol. 1999 Apr;32(2):403-14 – reference: 19319196 - PLoS One. 2009;4(3):e4982 – reference: 23516199 - Genome Announc. 2013 Mar 14;1(2):e0006513 – reference: 21918907 - Eur J Clin Microbiol Infect Dis. 2012 Jun;31(6):981-9 – reference: 22914622 - Sci Transl Med. 2012 Aug 22;4(148):148ra116 – reference: 11897067 - Emerg Infect Dis. 2002 Feb;8(2):160-6 – reference: 23169997 - MBio. 2012;3(6). pii: e00224-11. doi: 10.1128/mBio.00224-11 – reference: 8335346 - Infect Immun. 1993 Aug;61(8):3164-74 – reference: 23302790 - Virulence. 2013 Feb 15;4(2):107-18 – reference: 24099919 - Clin Infect Dis. 2014 Jan;58(2):225-32 – reference: 24236613 - Microb Drug Resist. 2014 Apr;20(2):150-5 – reference: 16704562 - J Intern Med. 2006 Jun;259(6):606-14 – reference: 19447910 - J Bacteriol. 2009 Jul;191(14):4492-501 – reference: 15276215 - Gene. 2004 Aug 4;337:189-98 – reference: 19143563 - J Infect Dis. 2009 Feb 15;199(4):513-21 – reference: 10376834 - Microbiology. 1999 May;145 ( Pt 5):1181-90 – reference: 3532983 - Arch Intern Med. 1986 Oct;146(10):1913-6 – reference: 22039542 - PLoS One. 2011;6(10):e26734 – reference: 2643575 - Infect Immun. 1989 Feb;57(2):546-52 – reference: 15501752 - Infect Immun. 2004 Nov;72(11):6262-70 – reference: 20887813 - Nanomedicine. 2011 Feb;7(1):88-96 – reference: 6295467 - Biochemistry. 1982 Dec 7;21(25):6503-8 – reference: 6455965 - Annu Rev Biochem. 1981;50:715-31 – reference: 21158655 - N Engl J Med. 2010 Dec 16;363(25):2377-9 – reference: 20376388 - Nat Prod Rep. 2010 May;27(5):637-57 – reference: 21576334 - Infect Immun. 2011 Aug;79(8):3309-16 – reference: 1872659 - Arch Intern Med. 1991 Aug;151(8):1557-9 – reference: 24476533 - J Proteome Res. 2014 Mar 7;13(3):1397-404 – reference: 19342674 - J Immunol. 2009 Apr 15;182(8):4947-56 – reference: 379572 - Microbiol Rev. 1978 Mar;42(1):45-66 – reference: 19433545 - Infect Immun. 2009 Jul;77(7):2657-71 – reference: 17804665 - Microbiol Mol Biol Rev. 2007 Sep;71(3):413-51 – reference: 9636876 - Clin Infect Dis. 1998 Jun;26(6):1434-8 – reference: 21964731 - Microbiology. 2011 Dec;157(Pt 12):3446-57 – reference: 15531878 - Nature. 2004 Dec 16;432(7019):917-21 – reference: 17220312 - Infect Immun. 2007 Mar;75(3):1463-72
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Snippet	The siderophore aerobactin is the dominant siderophore produced by hypervirulent Klebsiella pneumoniae (hvKP) and was previously shown to be a major virulence...
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SubjectTerms	Animals Enterobactin - analogs & derivatives Enterobactin - metabolism Glucosides - metabolism Humans Hydroxamic Acids - metabolism Klebsiella Infections - microbiology Klebsiella pneumoniae - genetics Klebsiella pneumoniae - growth & development Klebsiella pneumoniae - metabolism Klebsiella pneumoniae - pathogenicity Male Mice Microbial Viability - drug effects Phenols - metabolism Siderophores - metabolism Thiazoles - metabolism Virulence Young Adult
Title	Aerobactin, but not yersiniabactin, salmochelin, or enterobactin, enables the growth/survival of hypervirulent (hypermucoviscous) Klebsiella pneumoniae ex vivo and in vivo
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