Differences in Collagen Fiber Diameter and Waviness between Healthy and Aneurysmal Abdominal Aortas

Collagen plays a key role in the strength of aortic walls, so studying micro-structural changes during disease development is critical to better understand collagen reorganization. Second-harmonic generation microscopy is used to obtain images of human aortic collagen in both healthy and diseased st...

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Bibliographic Details
Published in:Microscopy and microanalysis Vol. 28; no. 5; pp. 1649 - 1663
Main Authors: Niestrawska, Justyna A., Pukaluk, Anna, Babu, Anju R., Holzapfel, Gerhard A.
Format: Journal Article
Language:English
Published: New York, USA Cambridge University Press 01.10.2022
Oxford University Press
Subjects:
Abdomen
Algorithms
Amplitudes
Aneurysm
Aneurysms
Aorta
Biological Applications
Collagen
Contrast media
Extracellular matrix
Fibers
Microscopy
Second harmonic generation
Tortuosity
Waviness
aneurysm
collagen fibers
human abdominal aorta
waviness
diameter
second-harmonic generation microscopy
ISSN:1431-9276, 1435-8115, 1435-8115
Online Access:Get full text
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Summary:Collagen plays a key role in the strength of aortic walls, so studying micro-structural changes during disease development is critical to better understand collagen reorganization. Second-harmonic generation microscopy is used to obtain images of human aortic collagen in both healthy and diseased states. Methods are being developed in order to efficiently determine the waviness, that is, tortuosity and amplitude, as well as the diameter, orientation, and dispersion of collagen fibers, and bundles in healthy and aneurysmal tissues. The results show layer-specific differences in the collagen of healthy tissues, which decrease in samples of aneurysmal aortic walls. In healthy tissues, the thick collagen bundles of the adventitia are characterized by greater waviness, both in the tortuosity and in the amplitude, compared to the relatively thin and straighter collagen fibers of the media. In contrast, most aneurysmal tissues tend to have a more uniform structure of the aortic wall with no significant difference in collagen diameter between the luminal and abluminal layers. An increase in collagen tortuosity compared to the healthy media is also observed in the aneurysmal luminal layer. The data set provided can help improve related material and multiscale models of aortic walls and aneurysm formation.
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ISSN:1431-9276
1435-8115
1435-8115
DOI:10.1017/S1431927622000629