Impaired Exocrine Pancreatic Function Associates With Changes in Intestinal Microbiota Composition and Diversity

Changes in intestinal microbiome composition are associated with inflammatory, metabolic, and malignant disorders. We studied how exocrine pancreatic function affects intestinal microbiota. We performed 16S ribosomal RNA gene sequencing analysis of stool samples from 1795 volunteers from the populat...

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Published in:Gastroenterology (New York, N.Y. 1943) Vol. 156; no. 4; p. 1010
Main Authors: Frost, Fabian, Kacprowski, Tim, Rühlemann, Malte, Bülow, Robin, Kühn, Jens-Peter, Franke, Andre, Heinsen, Femke-Anouska, Pietzner, Maik, Nauck, Matthias, Völker, Uwe, Völzke, Henry, Aghdassi, Ali A, Sendler, Matthias, Mayerle, Julia, Weiss, Frank U, Homuth, Georg, Lerch, Markus M
Format: Journal Article
Language:English
Published: United States 01.03.2019
Subjects:
Bacteria
Biomarker
Pancreas
Microbiome
ISSN:1528-0012, 1528-0012
Online Access:Get more information
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Summary:Changes in intestinal microbiome composition are associated with inflammatory, metabolic, and malignant disorders. We studied how exocrine pancreatic function affects intestinal microbiota. We performed 16S ribosomal RNA gene sequencing analysis of stool samples from 1795 volunteers from the population-based Study of Health in Pomerania who had no history of pancreatic disease. We also measured fecal pancreatic elastase by enzyme-linked immunosorbent assay and performed quantitative imaging of secretin-stimulated pancreatic fluid secretion. Associations of exocrine pancreatic function with microbial diversity or individual genera were calculated by permutational analysis of variance or linear regression, respectively. Differences in pancreatic elastase levels associated with significantly (P < .0001) greater changes in microbiota diversity than with participant age, body mass index, sex, smoking, alcohol consumption, or dietary factors. Significant changes in the abundance of 30 taxa, such as an increase in Prevotella (q < .0001) and a decrease of Bacteroides (q < .0001), indicated a shift from a type-1 to a type-2 enterotype. Changes in pancreatic fluid secretion alone were also associated with changes in microbial diversity (P = .0002), although to a lesser degree. In an analysis of fecal samples from 1795 volunteers, pancreatic acinar cell, rather than duct cell, function is presently the single most significant host factor to be associated with changes in intestinal microbiota composition.
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ISSN:1528-0012
1528-0012
DOI:10.1053/j.gastro.2018.10.047