Loss of CBP acetyltransferase activity by PHD finger mutations in Rubinstein-Taybi syndrome

Disruption of one copy of the human CREB binding protein (CBP or CREBBP) gene leads to the Rubinstein-Taybi syndrome (RTS), a developmental disorder characterized by retarded growth and mental functions, broad thumbs, broad big toes and typical facial abnormalities. The CREB binding protein (CBP) is...

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Vydané v:	Human molecular genetics Ročník 12; číslo 4; s. 441
Hlavní autori:	Kalkhoven, Eric, Roelfsema, Jeroen H, Teunissen, Hans, den Boer, Annemieke, Ariyurek, Yavuz, Zantema, Alt, Breuning, Martijn H, Hennekam, Raoul C M, Peters, Dorien J M
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	England 15.02.2003
Predmet:	Acetyltransferases - metabolism Amino Acid Motifs Amino Acid Sequence Blotting, Western Chromosome Aberrations Cyclic AMP Response Element-Binding Protein - genetics Cyclic AMP Response Element-Binding Protein - metabolism Exons Heterozygote Humans Models, Genetic Molecular Sequence Data Mutation Plasmids - metabolism Reverse Transcriptase Polymerase Chain Reaction Rubinstein-Taybi Syndrome - genetics Sequence Homology, Amino Acid Transfection Tumor Cells, Cultured
ISSN:	0964-6906
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Shrnutí:	Disruption of one copy of the human CREB binding protein (CBP or CREBBP) gene leads to the Rubinstein-Taybi syndrome (RTS), a developmental disorder characterized by retarded growth and mental functions, broad thumbs, broad big toes and typical facial abnormalities. The CREB binding protein (CBP) is an essential transcriptional coactivator for many different transcription factors. CBP has the intrinsic ability to acetylate histones and other proteins, which is regarded as an important step in transcription activation. In vitro studies have shown that this enzymatic activity critically depends on the integrity of a plant homeodomain (PHD)-type zinc finger in the HAT domain of CBP. We therefore investigated whether PHD finger mutations are present in RTS patients. Mutational analysis of 39 patients revealed eight novel heterozygous mutations in the HAT domain of CBP, one of which alters a conserved PHD finger amino acid (E1278K), while a second mutation deletes exon 22, which encodes the central region of the PHD finger. Functional analysis of these RTS-associated PHD finger mutants showed that they lacked in vitro acetyltransferase activity towards histones and CBP itself and displayed reduced coactivator function for the transcription factor CREB. Importantly, in EBV-transformed lymphoblastoid cells from the exon 22 deletion patient we found approximately 50% less endogenous CBP HAT activity. These findings therefore underscore the functional importance of the PHD finger in vivo and imply that reduction of CBP HAT activity, as exemplified here by disruption of the PHD finger, is sufficient to cause RTS.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0964-6906
DOI:	10.1093/hmg/ddg039