Derivation of two induced pluripotent stem cell lines from a healthy control subject

Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4...

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Published in:Stem cell research Vol. 82; p. 103621
Main Authors: Zhang, Dan, Huang, Di, Larcher, Leon M., Zaw, Khine, Chen, Shang-Chih, Jennings, Luke, Lamey, Tina M., Thompson, Jennifer A., McLaren, Terri L., Chen, Fred K., McLenachan, Samuel
Format: Journal Article
Language:English
Published: England Elsevier B.V 01.02.2025
Elsevier
Subjects:
Cell Differentiation
Cell Line
Cellular Reprogramming
Embryoid Bodies - cytology
Embryoid Bodies - metabolism
Fibroblasts - cytology
Fibroblasts - metabolism
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Kruppel-Like Factor 4
Male
ISSN:1873-5061, 1876-7753, 1876-7753
Online Access:Get full text
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Summary:Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2024.103621