Preparation and in vitro evaluation of folate-receptor-targeted SPION-polymer micelle hybrids for MRI contrast enhancement in cancer imaging

Polymer-SPION hybrids were investigated for receptor-mediated localization in tumour tissue. Superparamagnetic iron oxide nanoparticles (SPIONs) prepared by high-temperature decomposition of iron acetylacetonate were monodisperse (9.27 ± 3.37 nm), with high saturation magnetization of 76.8 emu g(-1)...

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Veröffentlicht in:Nanotechnology Jg. 24; H. 1; S. 015603
Hauptverfasser: Mahajan, Shveta, Koul, Veena, Choudhary, Veena, Shishodia, Gauri, Bharti, Alok C
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England 11.01.2013
Schlagworte:
Cell Death - drug effects
Contrast Media
Dextrans - chemical synthesis
Dextrans - chemistry
Dextrans - toxicity
Dextrans - ultrastructure
Diagnostic Imaging - methods
Endocytosis - drug effects
Folate Receptor 1 - metabolism
HeLa Cells
Humans
Intracellular Space - drug effects
Intracellular Space - metabolism
Magnetic Resonance Imaging
Magnetite Nanoparticles - chemistry
Magnetite Nanoparticles - toxicity
Magnetite Nanoparticles - ultrastructure
Micelles
Microscopy, Confocal
Microscopy, Electron, Transmission
Neoplasms - diagnosis
Neoplasms - pathology
Polymerization
Polymers - chemical synthesis
Polymers - chemistry
Pyrenes - chemistry
Spectrophotometry, Ultraviolet
X-Ray Diffraction
ISSN:1361-6528, 1361-6528
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Zusammenfassung:Polymer-SPION hybrids were investigated for receptor-mediated localization in tumour tissue. Superparamagnetic iron oxide nanoparticles (SPIONs) prepared by high-temperature decomposition of iron acetylacetonate were monodisperse (9.27 ± 3.37 nm), with high saturation magnetization of 76.8 emu g(-1). Amphiphilic copolymers prepared from methyl methacrylate and PEG methacrylate by atom transfer radical polymerization were conjugated with folic acid (for folate-receptor specificity). The folate-conjugated polymer had a low critical micellar concentration (0.4 mg l(-1)), indicating stability of the micellar formulation. SPION-polymeric micelle clusters were prepared by desolvation of the SPION dispersion/polymer solution in water. Magnetic resonance imaging of the formulation revealed very good contrast enhancement, with transverse (T(2)) relaxivity of 260.4 mM(-1) s(-1). The biological evaluation of the SPION micelles included cellular viability assay (MTT) and uptake in HeLa cells. These studies demonstrated the potential use of these nanoplatforms for imaging and targeting.
Bibliographie:ObjectType-Article-1
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ISSN:1361-6528
1361-6528
DOI:10.1088/0957-4484/24/1/015603