Luminescent imaging of β-galactosidase activity in living subjects using sequential reporter-enzyme luminescence

We generated a sequential reporter-enzyme luminescence (SRL) technology for in vivo detection of β-galactosidase (β-gal) activity. The substrate, a caged D -luciferin–galactoside conjugate, must first be cleaved by β-gal before it can be catalyzed by firefly luciferase (FLuc) to generate light. As a...

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Bibliographic Details
Published in:	Nature methods Vol. 3; no. 4; pp. 295 - 301
Main Authors:	Wehrman, Thomas S, von Degenfeld, Georges, Krutzik, Peter O, Nolan, Garry P, Blau, Helen M
Format:	Journal Article
Language:	English
Published:	New York Nature Publishing Group US 01.04.2006 Nature Publishing Group
Subjects:	Analysis Animals Beta galactosidases beta-Galactosidase - analysis beta-Galactosidase - metabolism Bioinformatics Biological Microscopy Biological Techniques Bioluminescence Biomedical and Life Sciences Biomedical Engineering/Biotechnology Catalysis Cell Membrane Permeability Drugs Enzymatic analysis Gene expression Gene Expression Regulation Genes, Reporter Health aspects Life Sciences Light Luciferases - genetics Luciferases - metabolism Luminescent Measurements - methods Lymphoid Tissue - cytology Lymphoid Tissue - metabolism Lymphoid Tissue - ultrastructure Methods Mice Mice, Transgenic Proteomics Sensitivity and Specificity United States
ISSN:	1548-7091, 1548-7105
Online Access:	Get full text
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