Establishment of a Panel of Human Cell Lines to Identify Cellular Receptors Used by Enteroviruses to Infect Cells

Non-pathogenic natural and recombinant strains of human Enteroviruses are the subject of ongoing study with some strains having been approved for use as anticancer agents. The efficacy of oncolytic virotherapy depends upon identifying the receptor utilized by a specific strain for cell entry, and th...

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Bibliographic Details
Published in:International journal of molecular sciences Vol. 26; no. 3; p. 923
Main Authors: Sosnovtseva, Anastasiia O., Le, Thi Hoa, Karpov, Dmitry S., Vorobyev, Pavel O., Gumennaya, Yana D., Alekseeva, Olga N., Chumakov, Peter M., Lipatova, Anastasia V.
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 22.01.2025
MDPI
Subjects:
Cancer
Cell Line
Cells
Communication
CRISPR
CRISPR-Cas Systems
Drug resistance
Enterovirus - physiology
Genes
Genomes
HEK293 Cells
Humans
Infections
Mutation
Phylogenetics
Proteins
Receptors, Virus - genetics
Receptors, Virus - metabolism
Virus Internalization
Viruses
coxsackievirus
Enteroviruses
CRISPR/Cas9
viral phylogeny
echovirus
ISSN:1422-0067, 1661-6596, 1422-0067
Online Access:Get full text
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Summary:Non-pathogenic natural and recombinant strains of human Enteroviruses are the subject of ongoing study with some strains having been approved for use as anticancer agents. The efficacy of oncolytic virotherapy depends upon identifying the receptor utilized by a specific strain for cell entry, and the presence of this receptor on the surface of cancer cells. Accordingly, a rapid and straightforward approach to determining the enteroviral receptors is necessary for developing an effective patient-specific, virus-based cancer therapy. To this end, we created a panel of seven lines with double knockouts on the background of the HEK293T cell line, which lacks the IFNAR1 gene. In these lines, the main viral receptor genes, including PVR, CXADR, CD55, ITGA2, SCARB2, ICAM1, and FCGRT, were knocked out using the CRISPR/Cas9 system. The panel of lines was validated on twelve different Enteroviruses types, providing a basis for studying the molecular mechanisms of enterovirus entry into cells, and for developing new therapeutic strains.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms26030923